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Quick Search     Adv Search 2017 Vol. 24, No. 6 Published: 2017-02-28   4 Antibacterial experiment research on nano-silver/polyurethane coated new antibacterial activity of tracheal catheter in rats HUANG Xiao-xia；XU Cheng Objective To explore the antimicrobial ability of nano-silver/polyurethane-coated endotracheal tube in rats.Methods48 Sprague-Dawley(SD)male rats in SPF grade supplied from Shenzhen PKU-HKUST Medical Center were selected in January 2016.24 healthy rats performed with endotracheal intubation and implanted with nano-silver/polyurethane were coated new antibacterial activity of tracheal catheter were randomly selected as experimental group.During the same period,24 healthy rats performed with endotracheal intubation and inserted common tracheal catheter were selected as control group in random.The clump counted by bronchoalveolar lavage fluid was tested by plate culture counting method 12,24,48 h,and 72 h after surgeries.The biological membrane thickness formed by endotracheal tube in the two groups was observed by microscope.ResultsThere was no great difference in clump count by bronchoalveolar lavage fluid 12 h after surgeries(P＞0.05).In the experimental group,the clump count by bronchoalveolar lavage fluid 24,48 h, and 72 h after surgeries were both much less than those in the control group (P＜0.05).The biological membrane thickness formed by endotracheal tube 12 h and 24 h after surgeries in the experimental group was not displayed statistical difference(P＞0.05).In the experimental group,the biological membrane thickness was formed by endotracheal tube 48 h and 72 h after surgeries were greatly lower than those in the control group(P＜0.05).ConclusionIn comparison with the normal catheter,antimicrobial nano-silver/polyurethane-coated endotracheal tube obtains a stronger antibacterial and anti-biological membrane augmentation properties. 2017 Vol. 24 (6): 4-6 [Abstract] ( 157 ) HTML (32 KB)  PDF  (0 KB)  ( 30 ) 7 Experimental study of protective effect of low molecular weight heparin on hepatic injury induced by sepsis in rat PAN Yi；HE Fei-min；CHEN Jun-xi；XIA Zhi-hui；YAN Zhi-jie；WU Li-li；CHENG Ying ObjectiveTo explore the protective effect and mechanism of low molecular weight heparin in liver injury of septic rats.Methods50 male SD rats were randomly divided into control group (10 cases),model group (20 cases)and treatment group(20 cases).Cecal ligation puncture(CLP)was used to made sepsis model.Rats in model group were given tail vein injection saline,and the treatment group was given tail vein injection low molecular weight heparin.The level of serum alanine aminotransferase (ALT),aspartate aminotransferase (AST),prothrombin time (PT),activated partial thromboplastin time(APTT),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)of rats′serum was detected at different time points,and histopathological changes of liver were observed at 12 h.ResultsCompared with control group, the level of serum ALT,AST,PT,APTT,TNF-αand IL-6 in model group and treatment group at different time points were obviously increased (P＜0.01).Compared with model group,the level of serum ALT,AST,PT,APTT,TNF-α and IL-6 in treatment group at different time points(6 and 12 h)were decreased (P＜0.05).Histopathological changes of liver at 12 h in treatment group obviously improved than model group.ConclusionLow molecular weight heparin has protective effect on liver damage of septic rats,the mechanism may be related to anticoagulation and inhibition of release of inflammatory factors. 2017 Vol. 24 (6): 7-10 [Abstract] ( 165 ) HTML (42 KB)  PDF  (0 KB)  ( 40 ) 11 Determination of Econazole Nitrate related substances in Triamcinolone Acetonide Acetate and Econazole Nitrate Cream XIAO Meng-jie； XIAO Xue-cheng ；XUE Da-quan；CUI Kai-yu ；TU Hao ；WU Ying ；ZHU Ya-lian ObjectiveTo establish a HPLC method for the determination of related substances of Econazole Nitrate in Triamcinolone Acetonide Acetate and Econazole Nitrate Cream.MethodsThe ZORBAX Eclipse XDB-C18(4.6 mm× 100.0 mm,3.5 μm)column was adopted.The mobile phase consisted of a mixture of Methanol-0.077%solution of Ammonium Acetate (20:80)as mobile phase A,a mixture of Methanol-Acetonitrile (40:60)as mobile phase B,which was used as gradient elution.The detection wavelength was 225 nm.The flow rate was 1.0 ml/min,and the column temperature was 35℃.ResultsEconazole Nitrate was well separated from impurity.The calibration curves was linear in range of 0.2-100.0 μg/ml for Econazole Nitrate,the regression equation was y=15.6295x+4.5920 (r=0.999 97).ConclusionThis method is simple and feasible to determine the related substances of Econazole Nitrate in Triamcinolone Acetonide Acetate and Econazole Nitrate Cream. 2017 Vol. 24 (6): 11-14转18 [Abstract] ( 153 ) HTML (45 KB)  PDF  (0 KB)  ( 36 ) 15 Expression of IMP3 and Ki-67 in gastroenteropancreatic neuroendocrine neoplasm and its relationship with clinicopathological features LI You-heng ；XU Hong；ZENG Xun ObjectiveTo investigate the expression of IMP3 and Ki-67 in gastrointestinalopancreatic neuroendocrine neoplasm(GEP-NEN)and its relationship with clinicopathological features.Methods59 patients with GEP-NEN diagnosed from January 2010 to October 2016 in our hospital and other surrounding areas were collected.Immunohistochemistry was used to detect the expression of IMP3 and Ki-67 in patients′specimens.Clinical and pathological features were combined,and the results were statistically analyzed.ResultsThe positive rate of IMP3 in GEP-NEN was 81.36%(48/ 59).The differences of the expression in different tumor size,whether there was lymph node metastasis or not,different tumor stages and classifications were statistically significant(P＜0.05).There was no statistical significance in the expression of tumor in terms of whether there was distant metastasis or not(P＞0.05).The positive rate of Ki-67 in GEPNEN was 44.68% (26/59).The differences of the expression in different tumor size,whether there was lymph node metastasis or not,whether there was distant metastasis or not,different pathological grades and clinical stages were statistically significant(P＜0.01);the positive rate of the expression of IMP3 and Ki-67 was increased with larger tumor size,lymph node metastasis,higher pathological grades,and more advanced clinical stages.ConclusionThe expression of IMP3 and Ki-67 in GEP-NEN may be one of the reference factors for determining patients′prognosis.IMP3 may play an important role in the development and progression of GEP-NEN. 2017 Vol. 24 (6): 15-18 [Abstract] ( 197 ) HTML (36 KB)  PDF  (0 KB)  ( 40 ) 19 Diagnosis and treatment of IgG4 related digestive system diseases ZHAO Lan-min；SHI Zhen-dong IgG4 related digestive system disease is a chronic inflammatory and fibrotic disease mediated by autoimmune reaction,involving multiple digestive organs,characterized by elevated serum IgG4 and infiltration of IgG4 positive plasma cells in the affected area.Due to the lack of specificity of clinical manifestations,imaging and laboratory tests,it is easy to be misdiagnosed and mistreated.The disease has good response to hormones,but it is easy to relapse after withdrawal,and it is effective to use hormone or combined immunomodulator and rituximab.This review will summarize the diagnosis and treatment of the disease. 2017 Vol. 24 (6): 19-21 [Abstract] ( 154 ) HTML (37 KB)  PDF  (0 KB)  ( 36 ) 22 Research progress on antiviral effects and chemical basis of Glycyrrhiza LYU Zhi-qiang；LIU Xiao-feng；DING Lian-cui；WANG Hui-ming；PAN Dong-qi；TIAN Jing-zhen Glycyrrhiza is a common used tonic Chinese herbal tonics,it has inhibitory effect on a variety of viruses.This paper summarized the antiviral effects of Glycyrrhiza and material basis and found Glycyrrhiza was widely used in antivirus effects,but for substance basis and mechanism is not perfect,lack of unity.Through the collation of Glycyrrhiza antiviral and material basis of literature analysis,proposed by multi group,mechanism,much in the way of thinking of the research,for development into antiviral drugs provides the theoretical basis for more extensive. 2017 Vol. 24 (6): 22-25 [Abstract] ( 140 ) HTML (50 KB)  PDF  (0 KB)  ( 39 ) 26 Compliance study on the effect of community intervention on hyperlipidemia LIU Shi-xing ；LIU Min-ling ；ZHAO Ji-xuan ObjectiveTo investigate the compliance of the effect of community intervention on hyperlipidemia.MethodsFrom November 2007 to September 2011,202 patients with hyperlipidemia were selected as study object from our community health service station and divided into study group(n=99)and control group(n=103)according to admission order.Both groups were given lifestyle intervention,and the study group was given lifestyle strengthening-based community comprehensive intervention.The awareness rate of disease,levels of blood lipids [triglyceride(TG),total cholesterol (TC),low-density lipoprotein (LDL),high-density lipoprotein (HDL)]and the incidence rates of hyperlipidemia associated cardiovascular and cerebrovascular diseases were compared between two groups.ResultsBefore intervention,the awareness rate of disease,TG,TC,LDL and HDL level showed no significant differences between two groups(P＞0.05).After intervention,the awareness rate of disease and HDL level was slightly higher,while TG,TC and LDL was lower than that before intervention,the awareness rates of disease in the study group was significantly higher than that in the control group （P＜0.05）.The increasing amplitude of HDL level [(1.58±0.54)mmol/L]and decreasing amplitudes of levels of TG[(2.75±0.89)mmol/L],TC[(3.35±0.99)mmol/L]and LDL[(2.42±0.66)mmol/L]in the study group was more obvious than that in the control group [(0.96±0.48)mmol/L,(3.20±1.04)mmol/L,(5.41±1.05)mmol/L, (3.98±0.74)mmol/L,] (P＜0.05).The incidence rate of hyperlipidemia associated cardiovascular and cerebrovascular diseases in the study group(5.05%)was significantly lower than that in the control group (24.27%),and the difference was statistially significant (P＜0.05).ConclusionCommunity comprehensive intervention can effectively improve the awareness rate of disease in patients with hyperlipidemia,and effectively regulate blood lipid levels,reduce the incidence of hyperlipidemia associated cardiovascular and cerebrovascular diseases. 2017 Vol. 24 (6): 26-28转31 [Abstract] ( 154 ) HTML (35 KB)  PDF  (0 KB)  ( 29 ) 29 Analysis the perioperative prophylactic use of antibiotics YA NG Qing-lian ObjectiveTo explore the clinical effect of perioperative prophylactic use of antibiotics and to analyze the application value.Methods300 surgical patients of Department of General Surgery in our hospital from March 2015 to September 2016 were selected as study objects.All patients were given prophylactic antibiotics during perioperative period.The incidence of incision infection and the length of hospital stay were recorded,and the infection rate of different types of incision and the methods of drug use were compared and analyzed.ResultsIncidence of postoperative incision infection and average length of hospital stay of 300 patients was respectively 5.33%and （6.1±1.0）d.In general,the infection rate of typeⅢ incision infection was significantly higher than that of typeⅡand typeⅠ,the infection rate of typeⅡincision infection was significantly higher than that of typeⅠ,with statistical difference(P＜0.05).The incidence rate of incision infection in the triple drug combination was significantly higher than that in the two drug combination and the simple medicin,the incidence rate of incision infection in the two drug combination was significantly higher than that in the simple medicin,with statistical difference (P＜0.05).ConclusionProphylactic use of antibiotics during perioperative period is helpful to reduce the rate of postoperative incision infection and the recovery rapid of patients. However,in order to obtain a more ideal effect,the appropriate medication method and medication time should be chosen according to incision type. 2017 Vol. 24 (6): 29-31 [Abstract] ( 149 ) HTML (31 KB)  PDF  (0 KB)  ( 41 ) 32 Retrospective analysis of misdiagnosed cases of herpes zoster TANG Gong-zheng ObjectiveTo explore the causes of misdiagnosis of herpes zoster and to provide a reliable reference for improving the accuracy of clinical diagnosis of herpes zoster.Methods150 patients with herpes zoster misdiagnosed as other diseases in our hospital from January 2013 to December 2015 were selected as study objects.The clinical data of 150 patients with herpes zoster were analyzed retrospectively.The causes of misdiagnosis were analyzed.ResultsAmong 150 herpes zoster misdiagnosed cases,the proportion of elderly patients accounted for 47.33%,the proportion of young and middle-aged patients accounted for 52.67%,and the difference was not statistically significant(P＞0.05).The main sites of misdiagnosis was trigeminal nerve,cervical ganglion,thoracic nerve and lumbosacral nerve,and the proportion was respectively 28.67%,30.67%,20.67%,20.00%;the main diseases misdiagnosed was trigeminal neuralgia,intercostals neuralgia,glaucoma,acute otitis media,tympanic membrane perforation,and the proportion was respectively 12.00%, 11.33%,10.00%,8.00%and 7.33%.The main reason of misdiagnosis was that early symptoms of herpes zoster were not typical,for the first time the doctors′diagnosis process had problems.The misdiagnosed cases were given symptomatic treatment after further diagnosis.After treatment,139 patients with pain symptoms disappeared or significantly reduced, and the symptoms of skin discomfort disappeared in 11 patients who did not appear pain symptoms.ConclusionIn the clinical diagnosis of herpes zoster,clinicians should improve their professional knowledge and conduct a comprehensive judgment by comprehensive examination,pay attention to the differential diagnosis of herpes zoster and other similar clinical symptoms of disease,as far as possible to reduce the incidence of misdiagnosis. 2017 Vol. 24 (6): 32-34 [Abstract] ( 153 ) HTML (32 KB)  PDF  (0 KB)  ( 35 ) 35 Value of early intervention after skin flap transplantation by hyperbaric oxygen treatment LI Run-chao； XIONG Bing-gang ；CHEN Jian； LI Ming-hua； OU Han-jie ObjectiveTo investigate the clinical value of HBO treatment on skin flap after transplantation with early intervention.Methods60 cases with skin flap transplantation operation treated in our department from June 2011 to April 2015 were selected as the research objects,and they were divided into two groups according to different therapeutic methods.30 patients were treated with additional HBO after transplantation of skin flaps and 30 patients as control were treated with conventional method alone.Flap transcutaneous oxygen saturation,temperature and flap survival rate were measured in two groups.ResultsThe flap transcutaneous oxygen saturation and temperature in observation group were significant higher than those in control group(P＜0.05).The flap survival rate of observation group was obviously higher than the control group(93.4%vs.73.3%,P＜0.05).HBO treatment was conducted within 48 h after operation, the flap survival rate was 100.0%,and it ws significantly higher than that after 48 h operation(71.4%)(P＜0.05).Conclusion Treatment of HBO has been done early after operation can improve survival rate of transplanted skin flap,HBO treatment conducted within 48 h after operation is the best intervention time. 2017 Vol. 24 (6): 35-37 [Abstract] ( 149 ) HTML (33 KB)  PDF  (0 KB)  ( 33 ) 38 Clinical effect of Mayinglong Shexiang Zhichuang Ointments combined with Calcium Dobesilate in treatment of thrombosed hemorrhoids ZHANG Bao-yin ObjectiveTo eveluate the clinical effect of Mayinglong Shexiang Zhichuang Ointments combined with Calcium Dobesilate in the treatment of thrombosed hemorrhoids.Methods196 cases with thrombotic hemorrhoids treated in our hospital from June 2012 to May 2016 were selected and randomly divided into the observation group (n=98)and control group (n=98).The treatment group was treated with Mayinglong Shexiang Zhichuang Ointments combined with Calcium Dobesilate,and the control group was only treated with Mayinglong Shexiang Zhichuang Ointments,and each ointment had 2.5 g,1 ointment was used sooner or later,part of drug was used to squeeze into anus,another part was for external use..The clinical effect in the fifth day and the tenth day of treatment between two groups was observed.Results After five days and ten days treatment,the effective rate of the observation group was 82.7%and 91.8%respectively,and that of the control group was 67.3%and 75.5%,and there was a significant statistical difference (82.7%vs. 67.3%,P＜0.05,91.8%vs.75.5%,P＜0.01);between two groups.ConclusionThe effect of Mayinglong Shexiang Zhichuang Ointments combined with Calcium Dobesilate on treating thrombosed hemorrhoids is better than that of treating with Mayinglong Shexiang Zhichuang Ointments only. 2017 Vol. 24 (6): 38-40 [Abstract] ( 165 ) HTML (34 KB)  PDF  (0 KB)  ( 38 ) 41 Clinical effect of ultrasound guided popliteal vein puncture in treatment of lower extremity venous thrombosis HUANG You-qing； LIAO Bin； LI Liang-yu； HUANG Zhi-yong ObjectiveTo discuss the clinical effect of ultrasound guided popliteal vein puncture in treatment of lower extremity venous thrombosis.Methods88 patients with venous thrombosis of lower limb accepted treatments in our hospital from June 2014 to March 2016 were selected,and were divided into observation group and control group by the double chromosphere grouping method.In the control group,patients were treated with intravenous infusion of urokinase in the treatment of peripheral venous,while in the observation group,after ultrasound guided popliteal vein puncture and transcatheter thrombolysis,continuous infusion of Urokinase thrombolytic therapy.Then,the time of swelling and the hospitalization time,clinical effect and the incidence rate of complications were compared between two groups.ResultsThe time of swelling of(6.33±2.14)d and the hospitalization time of(8.04±2.37)d in the observation group,while in the control group,the time of swelling was(9.18±2.62)d and the hospitalization time was(12.55±3.16)d,and the difference was significant(P＜0.01);the total effective rate in the observation group was 97.73%,while in the control group was 81.82%, the difference was significant(P＜0.01).The incidence rate of complications in the observation group was 2.27%,the control group was 0,there was no significant difference between the two groups(P＞0.05).ConclusionThe clinical effect of ultrasound guided popliteal vein puncture in treatment of lower extremity venous thrombosis is better,which can shorten the time of swelling and the hospitalization time,and the incidence rate of complications is low,thus it is worthy to be promoted in clinical practice. 2017 Vol. 24 (6): 41-43 [Abstract] ( 156 ) HTML (31 KB)  PDF  (0 KB)  ( 46 ) 44 Application effect of multimodal analgesia in artificial hip replacement CHEN Xi-cong；ZHOU Guan-ming；LIU Shao-hua；PENG Jian-hong ObjectiveTo investigate the effect of multimodal analgesia in artificial hip replacement.Methods110 patients with artificial hip replacement in our hospital from January to December 2015 were selected as research objects and divided into observation group and control group by random number table method,55 cases in each group.Patients in the control group were given with intraoperative periarticular injection of Ropivacaine hydrochloride and postoperative self controlled intravenous analgesia pump,patients in the observation group were given with vein injection of Parecoxib and Ropivacaine hydrochloride,postoperative Parecoxib sodium,Tramadol hydrochloride and Celecoxib analgesia analgesia,pain was evaluated by visual analogue scale (VAS),pain,adverse reaction and rehabilitation exercise compliance returning to the ward,6,12,24,48,72 h after operation between two groups were compared.ResultsVAS scores returning to the ward,6,12,24,48,72 h after operation in the observation group was significantly lower than that in the control group,and the difference was statistically significant(P＜0.05).Postoperative nausea and vomiting in the observation group occurred in 6 cases,dizziness and headache of 2 cases,the adverse reaction rate was 14.5%,postoperative nausea and vomiting in the control group occurred in 7 cases,headache in 1 case,1 case of urinary retention,the adverse reaction rate was 16.4%,there was no significant difference between two groups(P＞0.05).Postoperative rehabilitation exercise compliance rate in the observation group was 96.4%,which was significantly higher than that in the control group (83.6%),and the difference was statistically significant(P＜0.05).ConclusionThe implementation of multimodal analgesia can effectively reduce the pain degree of patients with artificial hip replacement after operation,has good safety,patients can implement better rehabilitation exercise after operation,which is worthy of promotion in clinical application. 2017 Vol. 24 (6): 44-46 [Abstract] ( 164 ) HTML (34 KB)  PDF  (0 KB)  ( 39 ) 47 The effect of simple high fibular osteotomy and arthroscopic lavage debridement combined with high fibula osteotomy in the treatment of knee osteoarthritis XIONG Liang ；REN Hui-juan ObjectiveTo explore the effect of simple high fibular osteotomy and arthroscopic lavage debridement combined with fibula osteotomy in the treatment of knee osteoarthritis.MethodsFrom November 2014 to November 2015,64 cases treated in our hospital were divided into joint group (n=34)and simple group (n=30)according to the different treatment.The joint group adopted arthroscopic lavage debridement combined with high fibula osteotomy,and the simple group only adopted high fibula osteotomy.VAS pain score,AKS knee scores,patients′satisfaction rate and complications rate between two groups were compared.ResultsVAS score of two groups was significantly reduced (P＜0.05),and AKS score was significantly higher(P＜0.05)after operation than that before operation.1 month and 3 months after operation, VAS score of the joint group was lower than that of the simple group(P＜0.05),AKS score was higher than that of the simple group(P＜0.05).6,12 months after operation,VAS score and AKS score of two groups had no significant difference (P＞0.05).The patients′satisfaction of the joint group was higher than that of the simple group 3 months after operation (P＜0.05),but there was no significant difference of two groups 12 months after operation(P＞0.05).The complication rate within 12 months of two groups had no significant difference (P＞0.05).ConclusionThe above two kinds of treatment methods are effective in the treatment of knee osteoarthritis,but the combination of arthroscopic lavage debridement has no obvious gain on the long-term effect,the simple high fibular osteotomy has characteristics of relatively simple trauma,less cost and simpler operation,which is worth to be promoted. 2017 Vol. 24 (6): 47-49转95 [Abstract] ( 125 ) HTML (35 KB)  PDF  (0 KB)  ( 31 ) 50 Application of Sufentanil on postoperative intravenous analgesia DENG Xi-xi ObjectiveTo explore the effect of Sufentanil used in postoperative intravenous analgesia.MethodsA total of 66 patients who underwent thoracic surgery in the First People′s Hospital of Fuzhou City from May 2013 to June 2015 were selected and divided into two groups according to random number table method,33 cases in each group.Fentanyl was used for patient-controlled intravenous analgesia (PCIA)after surgery in the control group,while Sufentanil was used for PCIA after operation in the observation group,VAS score and Ramsay score at different time points,change of postoperative each index and the incidence of adverse reactions were compared between two groups.ResultsThe pain effect of two groups after treatment was significant,Ramsay score 4,8,12 h after operation,24 h actual dosage,PCA pressing times and the number of effective drug,postoperative recovery time and extubation time in the observation group was significantly better than that in the control group,and the difference was statistically significant(P＜0.05).There was significant difference in the nausea and vomiting scores and total adverse reaction rate between two groups(P＜0.05).ConclusionThe effect of postoperative intravenous analgesia with Sufentanil is more accurate,more safety and remarkable value. 2017 Vol. 24 (6): 50-52 [Abstract] ( 163 ) HTML (32 KB)  PDF  (0 KB)  ( 42 ) 53 Clinical analysis of Bupivacaine combined with Sufentanil for subarachnoid block in cesarean section CHEN Meng-meng ObjectiveTo analyze the clinical value of Bupivacaine combined with Sufentanil for subarachnoid block in cesarean section.Methods50 cases of parturients underwent cesarean section in our hospital from January 2015 to January 2016 were selected as research object and divided into study group and control group according to different methods of anesthesia,25 cases in each group.The study group received Bupivacaine combined with Sufentanil for subarachnoid block in cesarean section,the control group was given simple Bupivacaine for subarachnoid block in cesarean section,the incidence of adverse reactions,anesthetic effect and neonatal Apgar score of two groups were compared.ResultsThe total incidence rate of adverse reactions was 8.00%in the study group,which was lower than that in the control group(28.00%),and the difference was statistically significant(P＜0.05).The anesthesia total superior rate of the study group was significantly better than that of the control group,and the difference was statistically significant(P＜0.05).There was no significant difference in neonatal Apgar score between two groups(P＞0.05).ConclusionIn cesarean section,the implementation of Bupivacaine combined with Sufentanil in subarachnoid block has significant clinical value,which can effectively reduce the incidence rate of maternal adverse reactions and improve the anesthetic effect,and will not cause serious neonatal effects,it is worthy of clinical promotion. 2017 Vol. 24 (6): 53-54转58 [Abstract] ( 165 ) HTML (32 KB)  PDF  (0 KB)  ( 33 ) 55 Effect of continuous intraoperative pumping of Dexmedetomidine on cognitive function in elderly patients undergoing laparoscopic surgery WEI Jiang-fu；ZHANG Ai-min；CHEN Li-ni ObjectiveTo investigate the effect of intraoperative continuous infusion of Dexmedetomidine on cognitive dysfunction in elderly patients after laparoscopic surgery.Methods44 patients with laparoscopic radical gastrectomy underwent elective general anesthesia from January 2015 to June 2016 were randomly divided into Dexmedetomidine group and normal saline group.The loading dose of Dexmedetomidine was maintained at 0.8 μg/(kg·h)for 15 min before anesthesia induction,followed by 0.4 μg/(kg·h)of sedation during surgery and 40 min before the end of surgery in the Dex group.Ns group was given intravenous infusion of equal volume of saline.MAP,HR and SpO2value were recorded before tracheal intubation(T0),intubation(T1),at the beginning of surgery(T2),at 30 min(T3),10 min before extubation (T4),30 min after extubation(T5).The operation time,spontaneous breathing recovery time,extubation time and sober time were recorded.At 1 days(D0),after 1 day operation(D1),and postoperative day three(D2),the number of adverse reactions such as nausea,vomiting and dizziness were recorded.ResultsAt the T1,T2,T4and T5time points,MAP and HR(exception of T1)of Dex group were significantly lower than those of Ns group(P＜0.05).At the time of D1-D2,compared with Ns group, the incidence of POCD in Dex group was significantly lower than that in Ns group (P＜0.05).There was no significant difference in the incidence rate of nausea and vomiting,vertigo between the two groups(P＞0.05).ConclusionIntraoperative pumping of Dexmedetomidine during operation is conducive to intraoperative circulatory stability,reduce the use of anesthetic drugs,reduce the incidence of postoperative POCD,and does not increase the awakening time and adverse reactions in the intraoperative continuous injection of Dexmedetomidine. 2017 Vol. 24 (6): 55-58 [Abstract] ( 147 ) HTML (43 KB)  PDF  (0 KB)  ( 37 ) 59 Analysis on the application value of transvaginal ultrasonography in the physical examination of women before pregnancy WEI Xiao-dan ObjectiveTo discuss the application value and specific effects of transvaginal ultrasound examination in the physical examination of women before pregnancy.Methods650 women who were given transvaginal ultrasonography before pregnancy from June 2014 to June 2016 in our hospital were randomly selected.According to the age group,those women were divided into group A(under 25 years of age,89 cases),group B(25 to 29 years of age,229 cases),group C(30 to 34 years of age,258 cases),group D (more than 34 years of age,74 cases）.The clinical data were retrospectively analyzed,and the positive detection rate and disease detection rate were compared among the four groups of women before pregnancy.ResultsThe detection rate of transvaginal ultrasonography was high,of which 86 cases were uterine leiomyoma,accounting for 43%of all cases;73 cases were cervical cysts,accounting for 36.5%;pelvic effusion were in 14 cases, accounting for 7.0%;annex cysts were in 27 cases,accounting for 13.5%.A total of 200 cases were observed abnormal, and the remaining 450 cases of women in transvaginal ultrasound examination were without obvious diseases.ConclusionRegular health checkups for females can significantly improve the detection rate of disease.Early treatment of gynecological diseases can help prepare for pregnancy,and transvaginal ultrasound examination is simple,accurate,and harmless,which has a significant clinical value in women′s physical examination. 2017 Vol. 24 (6): 59-61 [Abstract] ( 132 ) HTML (32 KB)  PDF  (0 KB)  ( 37 ) 62 Diagnosis value of transvaginal ultrasonography and hysteroscopy in abnormal uterine bleeding after menopause ZHAO Yan； GUAN Yun-ping；XIANG Yu-shi ObjectiveTo investigate the diagnosis value of transvaginal ultrasonography and hysteroscopy in abnormal uterine bleeding after menopause.MethodsAltogether 120 patients with perimenopausal abnormal uterine bleeding who were treated in the hospital between September 2014 to May 2016 were selected to compare the sensitivity,specificity and accuracy of application of transvaginal ultrasound,hysteroscopy and transvaginal ultrasound plus hysteroscopy in the diagnosis of perimenopausal abnormal uterine bleeding.ResultsThe sensitivity,specificity and accuracy by using transvaginal ultrasound examinations of abnormal uterine bleeding were 84.00%,90.00%and 87.50%;the sensitivity, specificity and accuracy by using hysteroscopy were 86.00%,87.14%and 86.67%;the sensitivity,specificity and accuracy by using the combination of transvaginal ultrasound and hysteroscopy were 98.00%,97.14%and 97.50%,which was higher than that of single transvaginal ultrasound and hysteroscopy examination,with significant difference (P＜0.05).ConclusionTransvaginal ultrasound and hysteroscopy have high sensitivity,specificity and accuracy in the diagnosis of postmenopausal uterine bleeding with minor differences,and are complementary to each other.Combination of two examination methods can provide the clinical basis of imaging and morphology,as a result of which it has high diagnostic effectiveness clinically. 2017 Vol. 24 (6): 62-64 [Abstract] ( 132 ) HTML (34 KB)  PDF  (0 KB)  ( 37 ) 65 Application value of high frequency color Doppler ultrasound,molybdenum target and their combination in the diagnosis of stageⅠ-Ⅱa breast cancer ZHENG Hua-ying；ZHU Yu-li；CHEN Xiao-cui；WU Wei-xiong ObjectiveTo study the clinical value that high frequency colour Doppler ultrasound and molybdenum target and their combination inⅠ-Ⅱ a period of breast cancer diagnosis.Methods96 cases confirmed by surgery pathology for early breast cancer patients from October 2010 to October 2016 in our hospital were selected as the research object, the results of high frequency color Doppler ultrasound and molybdenum target X-ray radiography were retrospectively analyzed,stage of breast cancer were done at the same time.According to the results of pathological diagnosis,the positive rate of diagnosis and the coincidence rate of the two methods were calculated.ResultsThe positive rate of high frequency color Doppler ultrasound,molybdenum target radiography alone and their combination in the diagnosis of breast cancer were 82.29%,71.88%and 90.63%,the positive rate of combined diagnosis was higher than that of single diagnosis,with significant difference (P＜0.05).The total coincidence rate of combined diagnosis was 88.54%,which was higher than that of high frequency color Doppler ultrasound and molybdenum target(79.17%,64.58%respectively),with significant difference (P＜0.05).ConclusionHigh frequency color Doppler ultrasound and molybdenum target are reliable methods in the diagnosis of breast cancer,combined use of two methods in the diagnosis of breast cancer,not only can improve the diagnostic positive rate,but also can provide the basis for the accurate staging of breast cancer,which is helpful to improve the treatment and prognosis of early breast cancer. 2017 Vol. 24 (6): 65-67 [Abstract] ( 171 ) HTML (33 KB)  PDF  (0 KB)  ( 37 ) 68 Application value of contrast-enhanced ultrasound in chest wall tuberculosis puncture biopsy SONG Dong-mei ObjectiveTo summarize the positive rate of biopsy after contrast-enhanced ultrasound,and to explore the application value of contrast-enhanced ultrasound in chest wall tuberculosis puncture biopsy.Methods70 patients with thoracic wall mass from November 2013 to December 2015 were selected,all patients were divided into the control group(30 cases)and the study group(40 cases)according to historical comparison method.The control group was used general ultrasound guided puncture biopsy,the study group was determined by ultrasound contrast to determine the area, then the ultrasound guided biopsy was performed.The sample complete rate and pathological positive rate were compared between the two groups.ResultsThe sample complete rate was 43.3%and pathological positive rate was 76.7% in the control group,which were respectively 87.5%and 100.0%in the study group.The sample complete situation in the study group was better than that in the control group,the pathological positive rate in the study group was higher than that in the control group,with significant difference(P＜0.05).ConclusionContrast enhanced ultrasound in puncture biopsy of chest wall tuberculous abscess can show the characteristics of chest wall tuberculosis blood supply,have higher sample complete rate and pathological positive rate,and have higher application value. 2017 Vol. 24 (6): 68-70 [Abstract] ( 129 ) HTML (31 KB)  PDF  (0 KB)  ( 34 ) 71 MRI diagnosis of chronic granulomatous mastitis YI Mei-zhi；LI Jin-song ；LIU Jin-cai ObjectiveTo explore the MRI features of chronic granulomatous mastitis(GM)and to improve the MRI diagnostic accuracy of the disease.MethodsThe clinical data and lesion morphology,signal,dynamic enhancement mode, time signal intensity curve (TIC)characteristics of MRI signs of 8 patients with chronic GM confirmed by pathology were retrospectively analyzed.All those patients were collected from January 2012 to October 2015 in our hospital.ResultsThe clinical findings of 8 patients with chronic GM were all lump in the breast.It tended to occur in unilateral side of breast,with unclear border,erythro swelling of mammary skin and enlarged axillary nodes.The lesions of 6 cases showed isointensity signals on T1WI,2 cases presented low signals.The high signals on fat suppression T2WI and DWI were recorded in 8 cases.The mastitis lesions were dynamic contrast enhanced,3 cases presented mass-like enhancement pattern and 5 cases presented non-mass-like enhancement pattern.Homogenous enhancement was found in 3 cases,thick-wall circular enhancement in 2 cases and thin-wall circular enhancement in 3 cases.3 cases of those lesions displayed clear edge and 5 cases showed ill-defined border.The time-signal intensity curves were pictured as typeⅠ, typeⅡ and typeⅢ in 4,2 and 2 cases respectively.2 cases were misdiagnosed with breast cancer.ConclusionChronic GM has no specific clinical appears,the combination with MRI findings is helpful to the diagnosis of chronic GM accurately and would contribute to provide a better therapeutic program for patients. 2017 Vol. 24 (6): 71-73转76 [Abstract] ( 148 ) HTML (32 KB)  PDF  (0 KB)  ( 31 ) 74 Effect of double-slice spiral CT combined with X-ray plain film in the diagnosis of lumbar disc herniation LIU Jun-zheng ；WANG Kai；ZHU Hai-long； ZHOU Zu-de ；ZHU Jian-gui ObjectiveTo explore the effect of double-slice spiral CT combined with X-ray plain film in the diagnosis of lumbar disc herniation.Methods86 cases of patients with lumbar disc herniation in our hospital from March 2014 to March 2016 were selected,who were used double-row spiral CT examination and X-ray examination.The diagnostic value of single diagnosis and combined diagnosis of the two examinations were compared,and the imaging features of lumbar disc herniation were summarized.ResultsCT examination showed that there were 12 cases of lumbar disc L3-4,46 cases of lumbar disc L4-5and 28 cases of lumbar disc L5-S1.Compared with the results of surgery,the coincidence rates of CT diagnosed as extralateral lumbar,pass,lateral and central type were 85.7%,88.9%,96.6%,100.0%respectively.X-ray examination showed that there were 7 cases of lumbar disc L3-4,19 cases of lumbar disc L4-5and 11 cases of lumbar disc L5-S1.Compared with the postoperative results,the coincidence rate of double-slice spiral CT and X-ray plain film examination was 100.0%,96.5%for CT examination and 43.0%for X-ray film examination,of which the combined diagnosis was obviously higher than those of single CT and X-ray plain film,with significant difference(P＜0.05).ConclusionThe diagnostic accuracy of double-slice spiral CT combined with X-ray plain film in the diagnosis of lumbar disc herniation is significantly higher than those of the single diagnostic accuracy,with higher clinical application value. 2017 Vol. 24 (6): 74-76 [Abstract] ( 171 ) HTML (33 KB)  PDF  (0 KB)  ( 39 ) 77 Different diagnostic value of transvaginal two-dimensional and three-dimensional ultrasonography in intrauterine lesions WU Guo-liang ；QIN Yi▲ ObjectiveTo compare the value of transvaginal two-dimensional and three-dimensional ultrasonography in the diagnosis of intrauterine lesions,to provide reference for clinical diagnosis.MethodsAltogether 182 cases of pathologically confirmed patients who were diagnosed as intrauterine lesions from January 2012 to December 2014 in our hospital were divided into two groups,90 cases who accepted preoperative transvaginal three-dimensional ultrasound examinations were selected as the observation group,92 cases who accepted preoperative transvaginal two-dimensional ultrasound examinations at the same period of time were selected as the control group.The specificity,sensitivity,accuracy,positive diagnosis rate and negative diagnostic rate of the two groups were compared.ResultsThe sensitivity,specificity,accuracy,positive predictive value and negative predictive value of the observation group in the diagnosis of endometrial hyperplasia and endometrial polyps,uterine fibroids mucosa by using three-dimensional ultrasound examinations was higher than those of the control group,with significant difference(P＜0.05).As for the diagnosis of endometrial cancer,the sensitivity and accuracy of the observation group was higher than those of the control group,with significant difference(P＜0.05),there was no significant difference in the the specificity,positive predictive value and negative predictive valuebetweenthetwogroups(P＞0.05).ConclusionTransvaginalthree-dimensionalultrasoundcandisplaycoronalsectionwith higher detection rate of intrauterine diseases than two-dimensional ultrasound,which has great clinical significance. 2017 Vol. 24 (6): 77-79 [Abstract] ( 109 ) HTML (34 KB)  PDF  (0 KB)  ( 36 ) 80 Efficacy of ganglioside in preventing and reducing chronic sensory neurotoxicity of Oxaliplatin YANG Hong-shan； LI Jun ；JIA Xiao-yan ObjectiveTo observe the efficacy of ganglioside in preventing and reducing chronic sensory neurotoxicity of oxaliplatin.Methods78 patients after gastric and colonrectal cancer resection from July 2014 to July 2016 of Department of Oncology,receiving oxaliplatin-based chemotherapy were divided into treatment group(n=40)and control group (n=38),the treatment group was given the ganglioside at the same time in each cycle of Oxaliplatin chemotherapy,the control group was used furan thiamine in each cycle of chemotherapy at the same time.21 days for a cycle in two groups.After chemotherapy,the neurotoxity evaluation,chemotherapeutic effect and adverse reactions were performed and compared.ResultsThere was noⅣ magnitude peripheral nerve toxicity in the two groups.TheⅡ andⅢ incidence of The peripheral nerve toxicity of experimental group was lower than that of control group(P＜0.05).The total effective rate of chemotherapy,the incidence of chemotherapy related adverse reactions,disease control rate,the median progressionfree surial were compared,there was no statistically significant difference（P＞0.05）.ConclusionGanglioside has better prevent the toxic effects of oxaliplatin into caused by peripheral nerve,and does not affect the curative effect of chemotherapy has not increased treatment-related adverse reactions. 2017 Vol. 24 (6): 80-82 [Abstract] ( 110 ) HTML (33 KB)  PDF  (0 KB)  ( 35 ) 83 Clinical effect of low molecular weight heparin for fetol growth restriction Pregnant women and perinatal outcome WU Guan ObjectiveTo investigate the clinical effect of low molecular weight heparin for fetal growth restriction pregnant women and perinatal outcome.Methods96 cases of FGR pregnant women in our hospital from November 2015 to July 2016 were randomly divided into research group and control group,48 cases in each group.The control group was treated with maternal underlying diseases,early strengthening of diet nutrition,while the research group was treated with low molecular weight heparin based on the treatment control group.The height of the uterus and fetal growth,amniotic fluid index,and biophysical score of pregnant women,and gestational age,weight and asphyxia of newborn,platelet count and coagulation function index changes of two groups were compared.ResultsAfter treatment,the height of the uterus, the growth value of head circumference,abdominal circumference,biparietal diameter and femur length of research group newborns were higher than those of control group(P＜0.05).The amniotic fluid index,and biophysical score of research group were(13.79±1.46)cm,(9.93±0.58)points respectively,which were better than those of control group of(11.41±1.50)cm, (9.26±0.42)points(P＜0.05).The newborns gestational age,weight of research group were better than those of control group (P＜0.05).Newborns asphyxia rate of research group was lower than that of control group(P＜0.05).The platelet count and coagulation function index of two groups had no significant difference(P＞0.05).ConclusionLow molecular weight heparin has exact effect for FGR.The weight of newborn is improved,and low molecular weight heparin could improve placental blood circulation,promote fetal growth,reduce the incidence of neonatal asphyxia.It′s worthy of wildly used. 2017 Vol. 24 (6): 83-85 [Abstract] ( 148 ) HTML (33 KB)  PDF  (0 KB)  ( 37 ) 86 Analysis of the outpatient prescriptions containing Voriconazole issued by department of hematology of People′s Hospital in Peking University LIU Yang ；FENG Wan-yu ObjectiveTo investigate the outpatient prescription containing Voriconazole issued by Department of Hematology in our hospital and analyze the application of Voriconazole so as to provide reference for clinical rational drug use and intervention on the irrational prescriptions.MethodsAll the outpatient prescriptions containing Voriconazole issued by Department of Hematology in our hospital from September 2015 to September 2016 were evaluated.Statistics of the use of Voriconazole and unreasonable prescription type were made,and the reasonable use of Voriconazole was also summarized.ResultsAmong 702 prescriptions containing Voriconazole,the average number of drugs per prescription was(2.03±0.58)kinds.The utilization rate of national basic drug was 24.1%.The average amount per prescription(5468.0±1062.1)yuan.Among 198 unreasonable prescriptions(accounting for 27.3%),there were 170 prescriptions without the indi cation of drug use(85.86%),7 with improper dosage(3.54%),20 with improper dosing frequency(10.10%)and 1 with improper combination of drugs(0.50%).ConclusionInterventions should be strengthened on the nonoccurrence of clinical indications,improper dosage and dosing frequency and inappropriate combination of medication in clinical use of voriconazole. 2017 Vol. 24 (6): 86-88转91 [Abstract] ( 136 ) HTML (39 KB)  PDF  (0 KB)  ( 38 ) 89 Effect of low dose Glucocorticoid in treatment of herpes zoster HUANG Wen-qing ObjectiveTo study the effect of low dose glucocorticoid in relieving herpes zoster pain and swelling.Methods88 patients with erpes zoster pain and swelling from February 2015 to July 2016 were selected as study objects,and were divided into two groups according to random number table method.The general group was used general antiviral drugs,neurotrophic drugs and analgesic drugs.The study group was used low dose glucocorticoid on the basis of general group.The two groups were treated for 7 days.The total effective rate,pain disappearance time,swelling relife time,scabby time and blister cessation time of patients with erpes zoster pain and swelling in two groups were compared.Before and after treatment,pain score,differences in scores of life quality and incidence rate of complications in two groups were compared.ResultsThe total effective rate of pain and swelling in study group was higher than that in general group(P＜0.05).All of pain disappearance time,swelling relife time,scabby time and blister cessation time in study group were less than those in general group(P＜0.01);After treatment,the study group had more improvement in pain score and quality of life score(P＜0.05).1 patient with pruritus and 1 patient with vomiting in study group,1 patient with vomiting in general group,and no serious complications were found in two groups.ConclusionLow dose glucocorticoid on pain and swelling of herpes zoster has exact effect,which can effectively improve the clinical symptoms of patients,promote pain and swelling disappear,accelerate wound scab,shorten the time of blister,with high safety.It is significant to improve the life quality of patients and worthy of popularization and application. 2017 Vol. 24 (6): 89-91 [Abstract] ( 110 ) HTML (32 KB)  PDF  (0 KB)  ( 32 ) 92 Technical essentials for molecular biological method on identification of Fritillaria cirrhosa and its decoction pieces YAN Hai-xia ；TAN Cheng； DU Xiao-wei；CHEN You-gen； FU Xin-tong TraditionalChinese Medicine is traditional medicine of china,which has extensive use in clinic treatment and daily health-care.The quality of Chinese medicinal materials and Traditional Chinese Medicine decoction pieces relates greatly to the clinical curative effect and security,which is also very important to the development of Traditional Chinese Medicine property.The identification of Chinese medicinal materials and Chinese medicinal decoction pieces is a focal point in traditional Chinese medicines profession,with the development of life science and technology leap,a variety of molecular biology technology is gradually used in medicinal plants and animals and the base source identification of Chinese Medicinal Materials,achieved good results.The"Pharmacopoeia of the People′s Republic of China" since 2010,contains the Chinese medicinal materials and medicinal slices(e.g,Agkistrodon,wu pin snakes and Fritillaria cirrhosa)molecular biology method,is to adopt new technology,new method to control the quality of traditional Chinese medicine.Standard has been four years,but brother units generally reflect the various technical problems encountered in the inspection process,it can′t solve.Identification based on the molecular biology of Fritillaria cirrhosa,for example,by summing up the main points that need to pay attention to the process of technology and common problems,aiming at will or are engaged in the inspection work colleagues to provide experience for reference,so that more efficient to complete the experiment successfully. 2017 Vol. 24 (6): 92-95 [Abstract] ( 125 ) HTML (41 KB)  PDF  (0 KB)  ( 34 ) 96 Content determination of camphor,menthol and phenol in compound spiritus camphorae by gas chromatography YANG Yue-long；GONG Li-min；LI Jing；HUANG Shao-guo ObjectiveTo establish a method for the content determination of camphor,menthol and phenol in compound spiritus camphorae by gas chromatography.MethodsThe capillary column in KB-WAX type(30.00 m×0.32 mm×0.25 μm) was used.Nitrogen was selected as the carrier gas with a flow rate of 3.0 ml/min.The programmed-temperature conditions by flame ionization detector(FID)were to start at the initial temperature of 130℃and keep for 10 min;to rise the temperature to 230℃at 5℃/min and keep for 5 min.The inlet temperature was 200℃and the temperature of detector was 250℃. It was determined by the external standard method.ResultsCamphor,menthol,phenol could be easily separated.The linearity ranges of camphor,menthol,and phenol were 0.2029-1.6232 mg/ml(r=0.9993,n=9),0.1964-1.5712 mg/ml(r=0.9995,n=9),and 0.2 074-1.6 592 mg/ml respectively.The average recoveries were 99.43%(RSD=0.85%),101.85%(RSD=0.84%)and 101.45% (RSD=0.53%)respectively.ConclusionThe method is simple and easy to perform,high in sensitivity,and good in reproducibility,which can be used for the content determination of camphor,menthol and phenol in compound spiritus camphorae. 2017 Vol. 24 (6): 96-99 [Abstract] ( 122 ) HTML (39 KB)  PDF  (0 KB)  ( 40 ) 100 Clinical effect of Weiwei Granules combined with auricular point pressing with bean in the treatment of atrophic gastritis NIU She-hui ObjectiveTo analyze the clinical effect of Weiwei Granules combined with auricular point pressing with bean in the treatment of atrophic gastritis.Methods100 patients with atrophic gastritis admitted into our hospital from January 2014 to March 2016 were selected.All patients were randomly divided into two groups,and there were 50 cases in each group.Control group was purely treated with Weiwei Granules,while observation group was treated auricular point pressing with bean on the basis of Weiwei Granules.The clinical effect,treatment score and incidence rate of adverse reaction between two groups after treatment were compared.ResultsThe curative effective rate of observation was 87.76%,which was significantly higher than of control group(74.47%),and there was a statistical difference(P＜0.05).After treatment,the symptoms,pathological score and gastroscope score of observation group were lower than control group,and there was statistical difference(P＜0.05).There was no statistical difference in the incidence rate of adverse reactions between two groups(P＞0.05).ConclusionWeiwei Granules combined with auricular point pressing with bean in the treatment of atrophic gastritis has significant clinical effect,and it can effectively relieve patients′diseases with less adverse reactions and high safety,which is worthy of clinical application and promotion. 2017 Vol. 24 (6): 100-102 [Abstract] ( 98 ) HTML (38 KB)  PDF  (0 KB)  ( 33 ) 103 Effect of acupuncture-moxibustion matching with Huanglian Ejiao Decoction treating perimenopausal insomnia SHAN Xing-yu ；HE Xiao-mei；LIU Jie-wen ObjectiveTo explore the effect of acupuncture-moxibustion matching with Huanglian Ejiao Decoction treating perimenopausal insomnia.MethodsEighty patients with perimenopausal insomnia treated in Traditional Chinese Medicine Hospital in Zengcheng District of Guangzhou City from May 2015 to March 2016 were randonly divided into control group(n=40)and observation group(n=40).In the control group,only Huanglian Ejiao Decoction was used for the treatment,while in the observation group,on the basis of the administration of Huanglian Ejiao Decoction,acupuncturemoxibustion was combined.The effect of improving traditional Chinese medicine (TCM)symptoms,quality of life,Pittsburgh sleep index (PSQI),HAMA and HAMD score were compared between two groups.ResultsThe effective rate of improving TCM symptoms in the observation group (95.00%)was higher than that in the control group accounting for 62.50%(P＜0.01).In the observation group,the score of quality of life was much higher than that in the control group(P＜0.05).In the observation group,after 1,2,and 4 weeks therapy,the score of PSQI,HAMA,and HAMD were all lower than those before treatment(P＜0.05)as well as in the control group after treatment(P＜0.05).ConclusionAcupuncture-moxibustion matching with Huanglian Ejiao Decoction treating perimenopausal insomnia obtains a remarkable effect.It can effectively improve patients′insomnia state as well as sleep quality,hence betters quality of life and poor mental state, which has a higher clinical application value. 2017 Vol. 24 (6): 103-105 [Abstract] ( 123 ) HTML (36 KB)  PDF  (0 KB)  ( 39 ) 106 Protective mechanism of phosphatidylinositol 3-kinase on myocardial ischemia/reperfusion injury induced by intermittent hypoxia in rats WANG Yuan-yuan；CAO Jian；WAN Jian-hua ObjectiveTo study the mechanisms of phosphatidylinositol 3-kinase PI3K on myocardial ischemia/reperfusion injury induced by intermittent hypoxia in rats.Methods60 rats were randomly divided into 4 groups,included sham-operated group(n=15),myocardial ischemia/reperfusion group(I/R)(n=15),I/R+intermittent hypoxia(IH)group (n=15)and I/R+IH+PI3K inhibitor group(n=15).Mice were exposed to intermittent hypoxia cycles to establish the IH model.I/R model was established by ligating the left anterior descending coronary artery,myocardial infarct areas were measured through TCC staining,the expression of phosphorylation of protein kinase B (Akt)was analyzed by Westen blot.ResultsCompared with the I/R group,the myocardial infarct size of IH+I/R group decreased (P＜0.05),the expression of Akt protein increased(P＜0.05).Compared with the IH+I/R group,the myocardial infarction area of IH+I/R+PI3K inhibitor group increased(P＜0.05),the expression of Akt protein decreased(P＜0.05).ConclusionThere is protective effects of intermittent hypoxia on myocardial ischemia injury in rats,and the protective mechanism is related to the activation of inibitor group PI3K signaling pathway. 2017 Vol. 24 (6): 106-108 [Abstract] ( 139 ) HTML (32 KB)  PDF  (0 KB)  ( 35 ) 109 Study of blood flows in main visceral organs of digestion and absorption in spleen yang deficiency and its underlying mechanism QIAO Hai-feng；LIU Wen-jun；LIU Xu-dong；WANG Ling-zhi；LIU Hui-hui；WANG De-shan；SHAN De-hong ObjectiveTo study blood flow changes of main visceral organs of digestion and absorption in spleen yang deficiency and its possible mechanism from the ratio of thromboxane A2(TXA2)and prostacyclin(PGI2).Methods16 SPF rats were randomly divided into the control and the spleen yangdeficiency group(the model group),8 rats in each group. Laser speckle was used to detect blood flow changes in liver,pancreas,stomach,small intestine(SI)and its mesenterium. ELISA was employed to measure serum thromboxane A2(TXA2)and prostacyclin(PGI2)concentrations.ResultsCompared with those in the control group,blood flows inliver left and right lobes,pancreas,stomach and small intestine and TXA2/ PGI2(T/A)value were significantly increased in the model group(all P＜0.01),but blood flow of SI mesenterium and serum PGI2level were decreased obviously in the model group (all P＜0.01).ConclusionThere is enhancement of blood flows in main visceral organs of digestion and absorption in spleen yang deficiency,and its underlying mechanism might be attributed to the increase of T/P value. 2017 Vol. 24 (6): 109-111 [Abstract] ( 107 ) HTML (39 KB)  PDF  (0 KB)  ( 34 ) 112 Analysis and report of ABO difficult blood group identification YANG Yong-huai；ZENG Yuan-yuan ObjectiveTo study the method and effect of identification of suspicious ABO blood group,to provide an important guarantee for the safety and effectiveness of clinical blood transfusion.MethodsFrom January 2012 to December 2015,83 cases of suspicious ABO blood samples in 37 264 blood specimens of blood donors were selected as the research object,the 83 suspicious ABO blood specimens were carried out serological detection,saline tube method,Polybrene method and absorption elution test of blood group positive and negative stereotypes were detected.The coincidence rate of saline tube method,saline tube method+Polybrene method,saline tube method+Polybrene method+absorption elution test of blood group positive and negative stereotypes were calculated,the inconformity reason of saline tube method in detecting suspicious ABO blood group positive and negative stereotypes was analyzed.ResultsIn the 83 cases of suspicious ABO blood samples,a total of 37 specimens of blood samples were consistent of positive and negative, and the coincidence rate was 44.58%,the coincidence rate of saline tube method and Polybrene method was 50.60%,the coincidence rate of saline tube Polybrene method+absorption elution test was 75.90%.The coincidence rate of combined detection of the three methods was higher than that of the single saline test tube method,the saline tube test method and the Polybrene method (P＜0.05).The main inconformity cause of saline tube test identification of ABO blood group were the AB subtype,weak A antigen,weak B antigen,irregular antibody positive.ConclusionThe saline tube method,Polybrene method,absorption elution test of combined detection of suspicious ABO blood group identification can accurately identify the positive and negative blood type. 2017 Vol. 24 (6): 112-114 [Abstract] ( 160 ) HTML (32 KB)  PDF  (0 KB)  ( 37 ) 115 Analysis for genotype and homology of carbapenemase resistance in Acinetobacter baumannii RAO Li-hua； TU Jian-bin； WANG Yan-qin ObjectiveTo analyze the genotype and homology of drug resistance gene in carbapenemase resistant Acinetobacter baumannii(CRAB).Methods68 strains of CRAB isolated from January 2013 to August 2014 were included in our retrospective analysis.Multiplex Polymerase Chain Reaction(Multiplex-PCR)was conducted to analyze six types of β-lactamase resistant gene IMP,VIM,OXA-23,OXA-24,OXA-58,OXA-51 in these strains of CRAB,and the gene homology of 8 CRAB isolateswere analyzed by pulsed field gel electrophoresis(PFGE).ResultsAll of the 68 isolates of CRAB were found to carry OXA-23,OXA-51,but not IMP,VIM,OXA-24,OXA-58．The result of PFGE suggested that the 8 CRAB isolates might derived from the same clone.And the gene homology analysis showed high homology among them.ConclusionAll the 68 CRAB strains isolated from our hospital belong to the same clone and carry OXA-23,OXA-51 genes mainly,indicating that there is clone spread among CRAB isolates. 2017 Vol. 24 (6): 115-117转131 [Abstract] ( 133 ) HTML (35 KB)  PDF  (0 KB)  ( 37 ) 118 Application value of immunosuppression and immune fluorescence quantitative method in the determination of creatine kinase isoenzyme CHEN Wei-jun； YE Chang-yuan； CHENG Xiao-ling ObjectiveTo explore the application value of immunesuppression and immune fluorescence quantitative method in the determination of creatine kinase isozyme activity.Methods70 patients in our hospital from January 2014 to December 2015 were selected and divided into two groups according to the disease type,with 35 cases in each group. The A group was acute myocardial infarction (AMI)and the duration was in 4-8 h;the B group was non-cardiac disease and high CK-MB activity.The CK activity and CK-MB activity of patients in two groups were respectively determinated using the velocity method and immunesuppression method,and the quality of CK-MB was determined using immune fluorescence quantitative method.ResultsThe CK activity[(585.0±102.0)U/L],CK-MB activity[(85.0±22.0)U/L]and quality of CK-MB[(18.5±2.2)ng/ml]of patients in group A were all raised;the CK activity of patients in group B was normal, the CK-MB activity was raised with a inconsistent results that the quality of CK-MB was not raised.ConclusionThe method to determinate the CK-MB activity in general is immunesuppression method.But because of the limitation of the methodology,the activity of CK-MB may falsely raise.On this occasion,doctors should combine the clinic and patients′conditions,using the immune fluorescence quantitative method to make further determination of the quality of CK-MB, in order to provide more accurate test results for the patients. 2017 Vol. 24 (6): 118-120 [Abstract] ( 104 ) HTML (28 KB)  PDF  (0 KB)  ( 32 ) 121 Application of blood routine test in the differential diagnosis of iron deficiency anemia and megaloblastic anemia TONG Li-jun ObjectiveTo explore the application value of blood routine test in the diagnosis and differentiation of commonly-seen anemia.MethodsFrom June 2014 to June 2016,80 patients with anemia treated in our hospital were selected as research objects and divided into two groups according to the type of anemia.40 patients with iron deficiency anemia were classified into the control group,and the rest 40 patients with megaloblastic anemia were categorized into the research group.The blood routine test in the two groups was used and the diagnostic effect was analyzed in the two groups.ResultsThe mean red cell blood volume,red blood cell distribution width,mean corpuscular volume,Hb,RBC/Hb and MCHC levels in the anemia patients in the research group was higher than that in the control group,the level of RBC was lower than that in the control group,with significant difference(P＜0.05).There was no difference in the clinical diagnostic accuracy between the two groups(P＞0.05).ConclusionBlood routine examination is of great value in the diagnosis and differentiation in common anemia such as iron deficiency anemia and megaloblastic anemia,etc.. 2017 Vol. 24 (6): 121-122转135 [Abstract] ( 137 ) HTML (31 KB)  PDF  (0 KB)  ( 34 ) 123 Application value of T cell spot test in the diagnosis of extrapulmonary tuberculosis GONG Mi；LU Ying ObjectiveTo discuss the diagnostic value of T cell spot test in the diagnosis of pulmonary tuberculosis.Methods200 cases of suspected pulmonary tuberculosis from January 2013 to August 2016 in our hospital were selected as the object of study review,all patients were detected by T cell spot test and tuberculosis antibody detection, clinical diagnosis result was set as reference,the diagnostic sensitivity,specificity and accuracy of extrapulmonary tuberculosis were calculated and compared between tuberculosis antibody and T cell spot test.ResultsThe diagnostic sensitivity,specificity and accuracy of T cells spot test in extrapulmonary tuberculosis were 96.45%,93.55%and 96.00%,and compared with the sensitivity(53.85%),specificity(64.52%)and accuracy(55.50%)in the diagnosis of tuberculosis antibody detection,the difference was statistically significant(P＜0.05).The consistency between the results of the T cell spot test for the diagnosis of extrapulmonary tuberculosis and the clinical outcome was Kappa=0.725,and the agreement between the results of TB antibody detection and clinical diagnosis was Kappa=0.435.ConclusionThe T cell spot test can be used for rapid and accurate diagnosis of pulmonary tuberculosis,it has significant diagnostic value in the diagnosis of pulmonary tuberculosis,which can be used as an important auxiliary diagnosis method for the diagnosis of pulmonary tuberculosis. 2017 Vol. 24 (6): 123-125 [Abstract] ( 121 ) HTML (31 KB)  PDF  (0 KB)  ( 38 ) 126 Resistance mechanism and homology of Enterobacter cloacae resistant to carbapenem XIE Feng-chun；HU Long-hua；NING Chang-xiu；JIN Ying-qing ObjectiveTo explore the resistance mechanism and homology of Enterobacter cloacae resistant to carbapenem.Methods60 separated strains of Enterobacter cloacae resistant to carbapenem from October 2014 to March 2016 of Clinical Laboratory from Nanchang Charity Hospital for Gynecology and Obstetrics,the Second Hospital Affiliated to Nanchang University and the First People′s Hospital of Fuzhou City were selected as study objects.Drug sensitivity test was used to analyze the resistance rate,modified Hodge test and EDTA synergy test were used to detected carbapenem,PCR was used to amplify resistance genes and determinate genotypes,PFGE was used to analyze Strain homology.ResultsThe resistance rate of 60 strains to antibiotics in the top 3 were Cefoxitin(100.0%),Ceftazidime(90.0%), Atreonam(90.0%)and Polymyxin B was the lowest(5.0%).The positive rate of modified Hodge test and EDTA synergy test were respectively 76.7%and 81.7%,and 39 strains of carbapenem gene were detected in the 60 strains,and 3 strains with KPC-2,24 strains with IMP-4,12 strains with IMP-8,45 strains with other β-lactamase gene;60 strains were divided into A-O by PFGE,20 different types of 59 strains and only 1 strain was not successful in type.Conclusion The resistance mechanism of Enterobacter cloacae are mainly with KPC and IMP gene. 2017 Vol. 24 (6): 126-128 [Abstract] ( 146 ) HTML (32 KB)  PDF  (0 KB)  ( 37 ) 129 The expression and clinical significance of serum biochemical indexes in patients with liver cirrhosis WEN Xiao-Yun；FANG Xian-song；XU Hui-rong ObjectiveTo study the application feasibility of serum biochemical tests of liver cirrhosis and the diagnosis of disease.MethodsFrom January 2013 to June 2016,50 cases confirmed liver cirrhosis and 50 healthy subjects in the hospital were selected as the research object,they were set as the observation group and the control group.All subjects underwent biochemical tests,serum bile acid(TBA),cholinesterase(CHE),aspartate aminotransferase(AST),alkaline phosphatase(ALP),total bilirubin(TBil),prealbumin(PAB),glutamate dehydrogenase(GLDH).Leucine aminopeptidase(LAP), alpha fucosidase(AFU),adenosine deaminase(ADA)and acid biochemical index were compared between two groups.ResultsAST,ALP,TBA,TBil,GLDH,LAP,AFU,ADA index in the observation group were significantly higher than that in the control group(P＜0.01),and PAB,CHE in the observation group were significantly lower than that in the control group (P＜0.01).ConclusionIn the clinical diagnosis of liver cirrhosis,patients are grven biochemical test,and the difference between the biochemical index and the health of people can make a diagnosis of the liver. 2017 Vol. 24 (6): 129-131 [Abstract] ( 155 ) HTML (30 KB)  PDF  (0 KB)  ( 38 ) 132 Application of PDCA circulation in the youth science fund project declaration management LI Qi；CHEN Jin；LIU Fu-quan ObjectiveTo investigate the application and feasibility of the PDCA mode in the National Nature Science Youth Science fund project,propose the constructive management advice for the National Natural Science Foundation of young talent cultivation project,promote the development of hospital technology.MethodsFilter young people,who meet the requirement,the declaration rate and winning bid rate from January 2012 to December 2014 were compared.The PDCA mode was applied to reason analysis,making management plan,establishing hospital young talent foundation,hospital research performance appraisal,supervision incentive mechanism,long-term mechanism and the National Natural annual youth counseling measures.After fund winning feedback,departments implement and collaborative supervision were summarized at final.ResultsAfter the application of PDCA,the declaration rate and winning bid rate of young people have reached the expected target.ConclusionThe application of PDCA obviously improves the rate of declaration and winning bid.It can be widely used in hospital scientific management. 2017 Vol. 24 (6): 132-135 [Abstract] ( 147 ) HTML (39 KB)  PDF  (0 KB)  ( 33 ) 136 Clinical study of PDCA circulation method for improving the quality of pharmacy management QIAN Jue ObjectiveTo explore the effect of PDCA circulation method on improving the quality of pharmacy management.MethodsThe outpatient pharmacy which did not use PDCA circulation method in our hospital from April to June 2014 were selected as the control group.The outpatient pharmacy during the time period after PDCA cycle management conducted from October to December 2014 was selected as the observation group.The score of the quality of pharmacy management was compared between the two groups.The inventory of drugs,the number of drug shortage,and the error rate of drug batch number management were observed in the two groups,and the time of allocating and checking a single prescription,the mistake rate of drug delivery,and patients′satisfaction were observed in the two groups.ResultsThe score of the quality of pharmacy management in the observation group was higher than that in the control group,and the inventory of drugs,the number of times of drug shortage,and the error rate of drug batch management were lower than those in the control group,the time of allocating and checking a single prescription and the mistake rate of drug delivery in the observation group were lower than those in the control group,the patients′satisfaction was higher than that in the control group,and the differences were statistically significant(P＜0.05).ConclusionPDCA circulation method can improve the quality of pharmacy management,reduce the error rate and mistake rate of drug application,and improve patients′satisfaction. 2017 Vol. 24 (6): 136-138转141 [Abstract] ( 104 ) HTML (35 KB)  PDF  (0 KB)  ( 39 ) 139 Discussion of information construction of archives management in hospital ZHANG Feng-lian Hospital archives management plays an important role in the operation of hospital.The information construction of hospital archives management is the key link in the process of hospital management modernization.The hospital file is a real record of medical and health activities,which not only plays an important role in the development of hospital itself,but also on the development of hospital health,such as improving the level of scientific research technology and promoting medical progress.With the rapid development of modern information technology and network technology,the information construction and network management of hospital archives gradually become a development trend.In this paper,the significance of the construction of hospital archives information,the construction of the problems in the process of such issues,try to put forward a number of hospital archives information construction proposals. 2017 Vol. 24 (6): 139-141 [Abstract] ( 117 ) HTML (36 KB)  PDF  (0 KB)  ( 38 ) 142 Application of PDCA cycle management method in the management of sterile items in operation room LUO Wei-fang；ZHANG Chao-hong；MAI Jin-nan；MA Wan-yi；LIN Ze-hua；LI Miao；HUANG Ying ObjectiveTo investigate the effects of PDCA cycle method for the management of sterile items of the operation room operation room.MethodsFrom January to June 2015,traditional method was used to manage the sterile items of operation room(control group),from July to December 2015,PDCA cycle method was used to manage the sterile items of operation room(observation group).The completion rate,overdue rate and replenishment rate of two groups were compared.ResultsThe completion rate,overdue rate and replenish rate of observation group were 100.0%,0.0%and 99.0%, respectivly,while those of controt group were 80.0%,9.0%and 85.0%,respectivly,the difference between the two groups was statistically significant(P＜0.05).ConclusionThe PDCA cycle method for the management of stenile items of operation room is superior to the trational methid. 2017 Vol. 24 (6): 142-144 [Abstract] ( 177 ) HTML (33 KB)  PDF  (0 KB)  ( 32 ) 145 Strengthening the construction of the large instrument sharing platform in key laboratory YUAN Xiao-huan；ZHANG Chun-lei；WU Yan；ZHANG Yu-fei；CHE Zhong-qiang The sharing operation mechanism of large precision instruments in key laboratory should be strengthened to integrate the existing resources of the university,fully expand the scope of open use and promote the improvement of the sharing level so as to create high-quality sharing resources.This paper focuses on the development of key laboratory as well as the demand of the university and society.It also provides reasonable layout design of large instruments in Key Laboratory of Mudanjiang Medical University,integrates the resources of the university and builds an open sharing system model,thereby further establishingan intelligent management plat form.As a consequence,the instrument platform can serve teaching and scientific research in a more scientific and standardized manner and also the development of the university and the local economic construction. 2017 Vol. 24 (6): 145-148 [Abstract] ( 153 ) HTML (39 KB)  PDF  (0 KB)  ( 40 ) 149 Design and application of an abdominal wall positioning scale based on CATIA modeling for gynecological surgeries assisted with Da Vinci robot LIU Yan-jiang；AN Ran-ran；LI Dong-qing；CHANG Ying ObjectiveTo design an abdominal wall positioning scale based on CATIA 3D design software for gynecological surgeries assisted with Da Vinci robot and to evaluate its effect in clinical surgeries.Methods60 cases of gynecological surgeries assisted with Da Vinci robot in Jilin Cancer Hospital from January 2015 to January 2016 were selected and divided into two groups(control group and experiment group)according to the hospital admission sequence,with 30 cases in each group.Firstly,CATIA 3D software was used to simulate the modeling,then the abdominal wall positioning scale was constructed and simulated according to the need of gynecologic robotic surgery and appropriate adjustments were made during the process of use.The conventional robotic surgical sheath card implantation method was taken in control group according to the experience of gynecologists;while,the gynecological abdominal wall robot positioning scale was used to position and implant the sheath card in experiment group.The time of abdominal puncture sheath card,the times of collision frequency of instrument arms for the bedside instrument arm system and improper surgical instrument angle,shift times of intraoperative sheath card and the satisfaction of gynecologists and nurses on surgical positioning were compared between the two groups.ResultsThe time of abdominal puncture sheath card in experiment group was shorter than that in control group,the times of collision frequency of instrument arms for the bedside instrument arm system and improper surgical instrument angle and the shift times of intraoperative sheath card in experiment group were less than those in control group,and the difference were statistically significant(t=29.46,P=0.000;t=15.66,P= 0.000;t=9.23,P=0.000;t=7.38,P=0.000);the satisfaction of gynecologists on surgical positioning method in experiment group was higher than that in control group,and the the difference was statistically significant(t=5.364,P=0.00);but there was no significant difference between the satisfaction of nurses on the two methods(t=1.756，P=0.086).ConclusionThe application of abdominal wall positioning scale for gynecological surgeries assisted with Da Vinci robot reduces the time used by doctors on abdominal puncture sheath,the collision frequency of instrument arms for the bedside instrument arm system and the exfoliation times of the sheath card from the abdominal wall,which improves satisfaction and achieves obvious clinical effect. 2017 Vol. 24 (6): 149-152 [Abstract] ( 121 ) HTML (42 KB)  PDF  (0 KB)  ( 38 ) 153 Epidemiological characteristics of hemorrhagic fever with renal syndrome in the Inner Mongolia Autonomous Region from 2011 to 2015 NAN Xiao-wei；HAN Song ；GUO Wei-dong ObjectiveTo understand the recent epidemic status of hemorrhagic fever with renal syndrome(HFRS)in the Inner Mongolia Autonomous Region in order to judge the trend of development of this disease and to establish measures andrecommendationsproposedfordiseasecontrol.MethodsThemonitoringdataofHFRSinthe Inner Mongolia Autonomous Region from January 2011 to December 2015 was statristically analyzed with Excel software.ResultsA total of 637 HFRS cases were reported,the average annual incidence was 0.51/100 000,and 7 deaths were reported with case fatality rate 0.56%in the Inner Mongolia Autonomous Region from 2011 to 2015.The HFRS cases mainly concentrated in the Hulunbeier City and 53 flags(county)had HFRS cases.The most infections concentrated in the autumn-winter season,the peak was still from October to December.The age of onset ranged from 30 to 65 years old.Farmer had the highest incidence in all occupations.ConclusionThe overall incidence of HFRS in the Inner Mongolia Autonomous Region showed a slight increased in 2014,2015.The risk of HFRS outbreak still exists.In these areas,the prevention and control should not be neglected,it is necessary to develop targeted prevention,adhere to the comprehensive prevention and control measures to give first place to vaccination and scientific deratization and rat-proofing,in order to achieve effective control of HFRS. 2017 Vol. 24 (6): 153-155 [Abstract] ( 141 ) HTML (30 KB)  PDF  (0 KB)  ( 39 ) 156 Analysis on the effect of disinfection and sterilization in medical institutions in Xigang District of Dalian City during 2012-2016 MA Yun-qiang；ZHAN Yang ObjectiveTo understand the disinfection and sterilization effect of monitoring medical institutions at all levels in Xigang district,so as to improve the quality of medical institutions,prevent and control nosocomial infection.MethodsStatistical methods was used to analyze the data of monitoring results of medical institutions at different levels in Xigang district from January 2012 to April 2016.ResultsFrom January 2012 to April 2016,a total of 1638 samples were dectected,the total qualified rate of all samples was 94.6%.Qualified rate of 2012,2013,2014,2015,2016 was 91.5%,98.8%,99.5%,95.7%,91.3%respectively (P＜0.05).Qualified rates of medical staff hands,air,object surface,pressure sterilizer,sterile packet detection,sewage,disinfectants and disinfection of endoscope was 89.9%,94.9%,99.3%,96.0%, 93.2%,88.6%,100.0%,100.0%,the difference in qualified rate of the medical staff hands,air,sterile packet detection and sewage each year was statistically significant(P＜0.05),qualified rate of object surface,pressure sterilizer,disinfectants and disinfection of endoscopes in each year was compared,the difference was not statistically significant(P＞0.05).Qualified rate in three hospitals at different levels of medical institutions was up to 92.3%,the lowest qualified rate of 31.2%in dental institutions,the monitoring unit qualified rate from high to low was respectively first-level hospital,second-class hospital and other medical units(except dental medical institution),the difference in qualified rate at different levels of monitoring unit detection was statistically significant(χ2=29.934,P＜0.05).ConclusionDisinfection and sterilization effect of medical institutions at all levels is good,but there are still potential danger of nosocomial infection,disinfection monitoring should be further strengthened,especially to the monitor of hand hygiene and sewage. 2017 Vol. 24 (6): 156-158 [Abstract] ( 104 ) HTML (32 KB)  PDF  (0 KB)  ( 40 ) 159 Study on effect of centralized treatment of endoscopic apparatus in CSSD HE Yong-hong；TAN Sheng-yan；LIU Yan-ping ObjectiveTo ensure validity of cleaning,disinfection,packaging and sterilization of endoscopic apparatus,and to effectively reduce the infection rate in hospitals and campuses,and to improve the quality of medical service security.Methods298 endoscopic apparatus altogether were chosen as research objects that including apparatus that were not carried out with the centralized treatment from June to December in 2014 and apparatus after the centralized treatment from January to June in 2015 in the Sixth Hospital Affiliated to Sun Yat-Sen University.The four main types of endoscopic apparatuses were handled by professional personnel by the means of large-scale equipment in the central sterile supply department(CSSD),which included cleaning,disinfection,packaging and sterilization.To evaluate the cleaning results,the qualified rate of these four types apparatuses were recorded respectively before and after the centralized treatment by means of naked-eye observation,magnifying-lens-aided check and occult blood test.ResultsBy the means of both naked eyes checking and magnifying-lens-aided check,the cleaning quality qualified rate of four types was 51.0% (188/298),99.2% (295/298)respectively before and after the centralized treatment.And the test result of occult blood was 39.0%(144/298),89.4%(259/298).Comparably,two test results showed that the four kinds of endoscopic apparatuses through the centralized treatment proved obvious effect in the process of cleaning,disinfection,packaging and sterilization effect,and the difference was statistically significant(P＜0.01).ConclusionIn the CSSD,four types of endoscopic apparatuses in the hospital have been handled by the means of centralized treatment,which included the process of cleaning,disinfection,packaging and sterilization.This centralized method can effectively increase the qualification rate of cleaning endoscopic apparatuses,thus guaranteeing medical safety of patients. 2017 Vol. 24 (6): 159-161转165 [Abstract] ( 122 ) HTML (36 KB)  PDF  (0 KB)  ( 39 ) 162 Exploration of behavior-oriented method combined with PBL in the diagnostics teaching of"outstanding doctor" MA Jian-mei；NI Jing；BAI Dan ObjectiveTo explore the value of behavior-oriented method combined with problem-based learning(PBL) teaching method applied to the first＂outstanding doctor＂class of our school in diagnostics teaching.Methods13 grade students of our school in 2015 were randomly selected as experiment group (n=60)with the class of outstanding doctor students,the same period clinical professional class (regular class students)as control group (n=180).The experiment group was given behavior-oriented method combined with PBL teaching method,while the control group was given the traditional teaching method,teaching effect was evaluated through the questionnaire survey and examination.ResultsThe average score of the experiment group was slightly higher than that of the control group in the final diagnosis theory examination and medical examination operation,but the difference was not statistically significant(P＞0.05);the average score of the experiment group was higher than that of the control group in history collection and auxiliary examination results,the difference was statistically significant(P＜0.05).The approval rating of various abilities after teaching in the experiment group was better than that in the control group,and the difference was statistical significance(P＜0.01).ConclusionBehavior oriented teaching combined with PBL teaching method in our＂outstanding doctor＂in diagnostics teaching can stimulate the students′creativity,cultivate the students′autonomous learning ability,but the promotion is difficult. 2017 Vol. 24 (6): 162-165 [Abstract] ( 114 ) HTML (37 KB)  PDF  (0 KB)  ( 29 ) 166 Application effect analysis of PBL teaching method in emergency nursing clinical teaching XUE Jin-liang；YU Zhan-ying ObjectiveTo study the real effect of problem-based learning (PBL)teaching method in emergency nursing clinical practice teaching.MethodsSixty undergraduate nursing interns from a School of Nursing of Inner Mongolia College were selected for the experiment,who were practicing at emergency department of the Fourth Hospital of Inner Monggol Autonomous Region from July 2015 to April 2016,according to the division of a baseline test sequence they were randomly divided into experimental group and control group,each group included 30 people.Experimental group was given PBL teaching method,and the control group was used by traditional teaching method,basic theory of emergency nursing,related skills,satisfaction of teaching method and teaching effect to teacher from emergency department 4 weeks after internship were compared between two groups.ResultsThe theory,operation test result and nursing medical records writing score of the experimental group was better than that of the control group(P＜0.05);the teaching satisfaction of the experimental group was higher than that of the control group (P＜0.05).ConclusionThe PBL teaching method has the feasibility to better closely integrated nursing theory and clinical practice,improves the ability of theory, operation,self-study,problem solving and so on various aspects,improves the quality of teaching and learning experience in the emergency department practice teaching,which is worth popularizing in clinical nursing teaching. 2017 Vol. 24 (6): 166-168 [Abstract] ( 132 ) HTML (34 KB)  PDF  (0 KB)  ( 32 ) 169 Evaluation of the effect of humanistic nursing for patients with schizophrenia CHEN Shao-fen； LIANG Feng-zhen ；LONG Shao-dong ；XIAO Yuan-wei ObjectiveTo explore the clinical effect of humanistic nursing care for patients with schizophrenia.MethodsAltogether 100 cases of schizophrenia patients who were treated in our hospital from February 2014 to January 2016 were selected and divided into traditional group and humanized group according to different nursing,50 cases in each groups.Additionally,the general data of all the patients were retrospectively analyzed.General nursing care intervention was taken for traditional group;while,humanistic nursing care was applied to humanized group.Mastery of schizophreniarelated knowledge and treatment compliance;pre-and post-nursing clinical positive symptoms,negative symptoms and total PANSS scale;different indicator scoreing of post-nursing quality of life were compared.ResultsThe mastery of schizophrenia-related knowledge and treatment compliance of humanized group were significantly higher than those of traditional group,with obvious differences(P＜0.05);the pre-and scores of post-nursing clinical positive symptoms,negative symptoms and total PANSS score of humanized group were significantly lower than those of traditional group,with obvious differences (P＜0.05);the different post-nursing quality-of-life indicator scores of humanized troup were significantly higher than those of traditional group(P＜0.05).ConclusionApplication of humanistic nursing care is effective for patients with schizophrenia,and can improve the patients′master degree of schizophrenia-related knowledge,treatment compliance,clinical symptoms as well as their quality of life,which is worthy of promotion. 2017 Vol. 24 (6): 169-171 [Abstract] ( 102 ) HTML (32 KB)  PDF  (0 KB)  ( 39 ) 172 Effect of routine skin care and Bei Youfen in the prevention of skin injury in the radiotherapy of nasopharyngeal carcinoma XU Li-mei；ZENG Yan-mei；LIAO Mei-lian ObjectiveTo explore the effect of routine skin care and Bei Youfen in the prevention of skin injury in the radiotherapy of nasopharyngeal carcinoma.Methods120 patients with nasopharyngeal carcinoma received radiotherapy from December 2014 to December 2015 were randomly divided into observation group and control group,60 cases in each groups.The patients in control group were given conventional skin care,the observation group was used Bei Youfen to prevent skin damage on the basis of control group,preventive effect of two groups were compared.ResultsWhen radiation treatment patients in the two groups with different degrees of skin lesions,there were 40 cases of gradeⅠskin lesion,18 cases of gradeⅡ skin lesion,2 cases of gradeⅢ injury in the observation group;12 cases of gradeⅠ injury in the control group,37 cases of gradeⅡ injury,10 cases of gradeⅢ injury,1 case of gradeⅣ injury,the observation group of patients with skin lesions was significantly lower than that of control group,the difference was significant(P＜0.05).ConclusionBei Youfen can prevent the skin injury in radiotherapy of nasopharyngeal carcinoma with good clinical effect and can protect the skin of patients effectively,it is worthy to be popularized in clinic. 2017 Vol. 24 (6): 172-174 [Abstract] ( 139 ) HTML (32 KB)  PDF  (0 KB)  ( 37 ) 175 Application effect of painless anesthesia combined with psychological induction in children with dental fear REN Xiao-yun ；WU Chong；CHEN Man-ling ObjectiveTo study the application effect of painless anesthesia combined with psychological induction on pediatric dental fear.Methods300 children were accepted painless anesthesia at Shaoguan Stomatological Hospital ranging from January 2015 to September 2016 were selected and randomly divided into three groups,painless anesthesia and psychological induction (group A),traditional manual needle injection and psychological induction (group B),and traditional manual needle injection alone (group C),100 cases in each group.The degree of pain and anesthesia effect among three groups in the course of treatment were compared.ResultsAmong three groups,the pain index of group A was(0.37±0.18)cm,which was significantly lower than that of group B of(2.53±0.41)cm and(4.98±0.73)cm in group C (P＜0.05).The anesthetic effective rate in the group A was 86%,better than that of 45%in group B and 12%in group C (P＜0.05).ConclusionIn the process of painless anesthesia,combination of psychological induction can significantly reduce the degree of fear in children,and improve the therapeutic effect,which is worthy of further application and promotion. 2017 Vol. 24 (6): 175-177转181 [Abstract] ( 136 ) HTML (37 KB)  PDF  (0 KB)  ( 28 ) 178 Effect of rehabilitation nursing on pulmonary functionsand quality of life in elderly patients with chronic obstructive pulmonary disease YU Miao ；LUO Ping； LUO Jun-biao ObjectiveTo explore the effect of rehabilitation nursing on pulmonary functions and quality of life in elderly patients with chronic obstructive pulmonary disease(COPD).MethodsAltogether 120 cases of COPD patients who were treated in our hospital from January 2015 to June 2016 in Department of Respiratory Medicinewere involved in this study and divided into control group and observation group according to the admission time number,60 cases in each group.The control group was treated with routine therapy and nursing;while,the observation group was adopted rehabilitation nursing measures.The changes of lung functions and quality of life were compared between the two groups.ResultsPost-intervention pulmonary function indexes such as FVC,FEV1,FVC/FEV1,PEFand MMEF of the patients in observation group were significantly improved than those before intervention(P＜0.05),compared with control group,the differences were significant(P＜0.05).Post-intervention 6 MWT of patients in observation group was significantly increased than that before intervention(P＜0.05),compared with control group,the difference was significant(P＜0.05).Post-intervention dimension scores of respiratory symptoms,activity limitation and the impact of disease in SGRQ of patients in observation group were significantly reduced (P＜0.05)in contrast with those before intervention and had statistically significant difference compared with those of control group (P＜0.05).ConclusionRehabilitation nursing measures can improve the lung functions,enhance exercise tolerance and improve the quality of life in elderly patients with COPD,which is of great value in clinical application. 2017 Vol. 24 (6): 178-181 [Abstract] ( 126 ) HTML (37 KB)  PDF  (0 KB)  ( 38 ) 182 Effect of personalized nursing intervention on quality of life after surgery of lumbosacral metastatic tumor QIU Si；CEN Qiao-dan ；GUAN Cai-yun； LI Yi-ting ObjectiveTo explore the effect of personalized nursing intervention on postoperative quality of life of lumbosacral metastases.MethodsA total of 79 patients undergone surgeries of lumbosacral metastatic bone tumor at appropriate time from May 2014 to January 2016 in our hospital were selected as observational subjects.According to the sequence of visiting time,they were divided into control group(n=42)and observation group(n=37).In the control group, routine care was used,while in the observation group,active personalized nursing was added on the basis of the routine care.The sleep quality and mental state scores were compared between the two groups before and after operation.ResultsBefore operation,there was no statistical difference between sleep quality in two groups (P＞0.05).After operation, the scores of each factor in the observation group were lower than that in the control group,with statistical differences (P＜0.05).The scores of SAS and SDS before operation were not displayed significant differences in two groups(P＞0.05). However,after operation,the scores of SAS,SDS in the observation group was lower than that in the control group,with statistical difference (P＜0.05).ConclusionIndividualized nursing intervention refers to a nursing program centered by individuals and evidenced by data in one-to-one,which is beneficial to timely adjust postoperative rehabilitation regimen and improve the disease prognosis,and is worthy of promotion in clinical nursing. 2017 Vol. 24 (6): 182-184 [Abstract] ( 146 ) HTML (32 KB)  PDF  (0 KB)  ( 40 ) 185 Effect of predictive nursing on reducing the incidence of nosocomial infection in hemodialysis patients with diabetic nephropathy CHEN Li；SU Xiao-yan； LIU Yu-feng ObjectiveTo explore the effect of predictive nursing on the incidence of nosocomial infection in hemodialysis patients with diabetic nephropathy.Methods200 patients with diabetes mellitus CKD-5 who underwent hemodialysis were enrolled in our hospital from January to November 2016.The patients were divided into control group and experimental group by random number method,100 patients in each groups.The control group was given general nursing care.the experimental group was given foreseeing care,each patient intervened for 3 months.The hospital infection status and nursing satisfaction were recorded.ResultsAfter 3 months of intervention,the incidence of nosocomial infection in the experimental group was 4%,which was significantly lower than that of control group of 12%.The satisfaction degree of the experimental group was 95%,was significantly higher than that of control group of 80% (P＜0.05).Conclusions Predictive care can reduce the incidence of nosocomial infection in patients with CKD-5 diabetes mellitus and may increase the acceptance of nursing care provided by the caregiver. 2017 Vol. 24 (6): 185-187 [Abstract] ( 129 ) HTML (35 KB)  PDF  (0 KB)  ( 31 ) 188 Effect of high quality nursing service on illness cognition and living quality in endometrial polyp patients with hysteroscopic surgery CHEN Xi-mei ObjectiveTo investigate the effect of high quality nursing service on illness cognition and living quality in endometrial polyp patients with hysteroscopic surgery.Methods80 endometrial polyp patients were admitted into our hospital from March to December 2016 were randomly divided into control group and observation group,40 cases in each group.Two groups were received hysteroscopic surgery,the control group were taken conventional nursing service, the observation group were given high quality nursing service.The illness cognition and living quality of two groups were compared.ResultsThe good rate of illness cognition in observation group was 97.50%,was significantly higher than that of control group of 80.00%(P＜0.05);the living quality in observation group after nursing was significantly better than that in control group(P＜0.05).ConclusionHigh quality nursing service plays an important role in increasing patients′illness cognition and living quality,which has promotional value. 2017 Vol. 24 (6): 188-190 [Abstract] ( 135 ) HTML (31 KB)  PDF  (0 KB)  ( 33 ) 191 Application effect of holistic hursing in postoperativepatients with intraspinal tumor WU You-jie ；LIU Yan ObjectiveTo explore the application effect of holistic nursing in patients with intraspinal tumor.MethodsClinical data of 60 patients received intraspinal tumor resection in our hospital from December 2015 to May 2016 were retrospectively analyzed.According to the random number table method,the patients were divided into control group(n= 30)and observation group (n=30).Conventional nursing was used in control group and holistic nursing was used in observing group.Neural function recovery,complications and nursing satisfaction degree in patients between two groups were compared.ResultsNeurological functional score of observing group was obviously better than that of control group (P＜0.05).The incidence rate of post-operation complications in observation group(6.67%)was obviously lower than that of control group (26.67%)(P＜0.05).Total satisfaction degree of nursing in observation group was higher than that of control group(P＜0.01).ConclusionHolistic nursing can effectively promote the recovery of neurological function,reduce postoperative complications,and improve the nursing satisfaction degree. 2017 Vol. 24 (6): 191-193 [Abstract] ( 144 ) HTML (36 KB)  PDF  (0 KB)  ( 44 ) 194 Application effect of psychological nursing intervention in patients with pre-hospital first-aid XIA Ming-shu；LIANG Yan；LIN Dong-mei ObjectiveTo explore the application effect of psychological nursing intervention in pre-hospital emergency care patients.MethodsEighty patients by pre-hospital care in our first aid center from February 2013 to March 2015 were selected and evenly divided into conventional group and intervention group by a random number table 40 cases in each group.In the conventional group,traditional pre-hospital care and nursing measures were applied,while in the intervention group,the psychological intervention was strengthened.The improvement of psychological anxiety and quality of life score before and after nursing intervention were observed in the two groups.ResultsThere was no significant difference in the score of degree of adaptability,communication,understanding of disease knowledge,and positive mental attitude between two groups before intervention (P＞0.05).After nursing intervention,the above-mentioned indexes was (22.51±0.49)points,(21.85±1.02)points,(21.00±0.58)points,and (20.23±1.04)points respectively in the intervention group,and it was much higher than those in the conventional group [(13.52±1.00)points,(12.54±1.01)points,(13.49± 1.05)points,and (15.41±1.09)points](P＜0.05).In the conventional group,score of the indexes including health conditions of the body after being discharged,daily living activity,psycho-emotion,and social integration were all lower than those in the intervention group (P＜0.05).ConclusionIn the process of pre-hospital care,medical staff should take positive intervention to patients with bad emotion,and most of them can greatly alleviate after intervention with a better score of quality of life. 2017 Vol. 24 (6): 194-196 [Abstract] ( 140 ) HTML (32 KB)  PDF  (0 KB)  ( 39 )

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