分子生物学方法鉴别川贝母及饮片的技术要点探析

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Abstract TraditionalChinese Medicine is traditional medicine of china,which has extensive use in clinic treatment and daily health-care.The quality of Chinese medicinal materials and Traditional Chinese Medicine decoction pieces relates greatly to the clinical curative effect and security,which is also very important to the development of Traditional Chinese Medicine property.The identification of Chinese medicinal materials and Chinese medicinal decoction pieces is a focal point in traditional Chinese medicines profession,with the development of life science and technology leap,a variety of molecular biology technology is gradually used in medicinal plants and animals and the base source identification of Chinese Medicinal Materials,achieved good results.The"Pharmacopoeia of the People′s Republic of China" since 2010,contains the Chinese medicinal materials and medicinal slices(e.g,Agkistrodon,wu pin snakes and Fritillaria cirrhosa)molecular biology method,is to adopt new technology,new method to control the quality of traditional Chinese medicine.Standard has been four years,but brother units generally reflect the various technical problems encountered in the inspection process,it can′t solve.Identification based on the molecular biology of Fritillaria cirrhosa,for example,by summing up the main points that need to pay attention to the process of technology and common problems,aiming at will or are engaged in the inspection work colleagues to provide experience for reference,so that more efficient to complete the experiment successfully.

Key words： Molecular biology Fritillaria cirrhosa Polymerase chain reaction-restriction fragment length polymorphism

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	Articles by authors
	YAN Hai-xia
	TAN Cheng
	DU Xiao-wei
	CHEN You-gen
	FU Xin-tong

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YAN Hai-xia,TAN Cheng,DU Xiao-wei, et al. Technical essentials for molecular biological method on identification of Fritillaria cirrhosa and its decoction pieces[J]. 中国当代医药, 2017, 24(6): 92-95.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I6/92

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