ABO疑难血型鉴定的分析与报告

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Abstract ObjectiveTo study the method and effect of identification of suspicious ABO blood group,to provide an important guarantee for the safety and effectiveness of clinical blood transfusion.MethodsFrom January 2012 to December 2015,83 cases of suspicious ABO blood samples in 37 264 blood specimens of blood donors were selected as the research object,the 83 suspicious ABO blood specimens were carried out serological detection,saline tube method,Polybrene method and absorption elution test of blood group positive and negative stereotypes were detected.The coincidence rate of saline tube method,saline tube method+Polybrene method,saline tube method+Polybrene method+absorption elution test of blood group positive and negative stereotypes were calculated,the inconformity reason of saline tube method in detecting suspicious ABO blood group positive and negative stereotypes was analyzed.ResultsIn the 83 cases of suspicious ABO blood samples,a total of 37 specimens of blood samples were consistent of positive and negative, and the coincidence rate was 44.58%,the coincidence rate of saline tube method and Polybrene method was 50.60%,the coincidence rate of saline tube Polybrene method+absorption elution test was 75.90%.The coincidence rate of combined detection of the three methods was higher than that of the single saline test tube method,the saline tube test method and the Polybrene method (P＜0.05).The main inconformity cause of saline tube test identification of ABO blood group were the AB subtype,weak A antigen,weak B antigen,irregular antibody positive.ConclusionThe saline tube method,Polybrene method,absorption elution test of combined detection of suspicious ABO blood group identification can accurately identify the positive and negative blood type.

Key words： ABO difficult blood group Identification Blood transfusion

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	YANG Yong-huai
	ZENG Yuan-yuan

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YANG Yong-huai,ZENG Yuan-yuan. Analysis and report of ABO difficult blood group identification[J]. 中国当代医药, 2017, 24(6): 112-114.

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http://dangdaiyiyao.com/EN/ OR http://dangdaiyiyao.com/EN/Y2017/V24/I6/112

[1]	马红丽，贠中桥，杨波，等.ABO疑难血型3步分析法实践应用[J].临床血液学杂志，2011，24（2）：75-77.
[2]	Yoneyama T，Hatakeyama S，TobisawaY，et al.Blood group antigen-targeting peptide suppresses anti-blood group antibody binding to antigen in renal glomerular capillaries after ABO-incompatible blood reperfusion[J].Transplantation，2013，95（3）：418-425.
[3]	伍钢，杜海林.ABO疑难血型鉴定方法的建立与应用[J].临床输血与检验，2015，17（1）：68-70.
[4]	胡晓玉，吴学忠，吕蓉，等.83例疑难ABO血型鉴定及分析[J].国际检验医学杂志，2013，34（18）：2396-2397.
[5]	华洪葳，宿军，杨春晴，等.54例无偿献血者ABO疑难血型检测结果分析[J].中国输血杂志，2015，28（12）：1509-1511.
[6]	黄璐，欧小懂，古力，等.ABO疑难血型鉴定情况分析[J].中国实验诊断学，2014，18（7）：1190-1191.
[7]	临床输血规范流程协作组.红细胞ABO疑难血型检测分析与记录流程[J].中国输血杂志，2014，27（2）：127-129.
[8]	王峰，赵蒲宇，兰炯采，等.ABO疑难血型三步分析法的临床应用[J].中国输血杂志，2012，25（5）：459-460.
[9]	王欣，刘泽林，卢博，等.血液病性贫血患者红细胞参数的分布规律及其相互关系[J].中国医药科学，2015，5（14）：171-173.
[10]	Berséus O，Boman K，Nessen SC，et al.Nessen et al.Risks of hemolysis due to anti-A and anti-B caused by the transfusion of blood or blood components containing ABO-incompatible plasma[J].Transfusion，2013，53（Suppl 1）：S114-S123.
[11]	兰炯采，陈静娴，马红丽，等.推荐ABO疑难血型三步分析法[J].中国输血杂志，2010，23（3）：165-168.
[12]	陈志忠，廖扬勋，陈尚良，等.PCR-SSP基因分型技术在ABO疑难血型定型中的应用[J].现代检验医学杂志，2011，26（4）：53-55.
[13]	钟青，许业栋.骨髓形态检验在难治性贫血与巨幼细胞性贫血鉴别诊断中的临床价值[J].中国医药科学，2016，6（7）：172-174.
[14]	苏明珍，黄劲柏，陆雯静，等.轻型α地中海贫血与缺铁性贫血的临床鉴别[J].中国医药科学，2015，5（12）：10-12.
[15]	夏富益.红细胞ABO疑难血型检测分析与处理方案[J].当代医学，2015，21（35）：77-78.
[16]	范瑾，张珏.ABO血型鉴定中正反定型不一致时的分析与探讨[J].上海医药，2013，34（17）：37-39.
[17]	杨松.南充市无偿献血者ABO定型困难原因分析[J].川北医学院学报，2016，31（4）：579-581.
[18]	汪芳.疑难血型的临床分析和处理方法[J].国际检验医学杂志，2013，34（23）：3254-3255.