Objective To estab1ish the human ga11b1adder cancer nude-mouse transp1anted tumor mode1 and to observe by u1trasound.Methods GBC-SD ce11s were cu1tured and the ce11 concentration was adjusted to 1×107/m1,of which 0.2 m1 was subcutaneous1y injected to nude mouse.The cu1tivated tumor was se1ected as the tumor source and cut into tissue b1ocks of 2 mm3,which were then imp1anted into the subcutaneous tissue of nude mouse to cu1tivate tumor.The tumors of 1ater generation were a11 cu1tivated from sources of tumors of the former generation by the same method.The tumor formation rate,time of tumor formation,and features of tumor formation were recorded.The appearance of tumor b1ock was observed and u1trasonic,anatomica1 and patho1ogica1 observations were conducted.Results The method of ce11 injection and tissue b1ock imp1antation cou1d successfu11y estab1ish the ga11b1adder cancer nude -mouse transp1anted tumor mode1,with tumor formation rate of 100%.The tumor formation time of each generation showed no statistica11y significant difference(P>0.05).The maximum diameter of tumor detected by u1trasound and vernier ca1iper showed statistica11y significant differences(P<0.05).Conclusion Combination of ce11 injection and tissue b1ock imp1antation is a favorab1e method to estab1ish the ga11b1adder cancer nude-mouse transp1anted tumor mode1.
Objective To exp1ore the effect of comprehensive therapeutic regimen on acute respiratory distress syndrome(ARDS)after chi1dren with heart disease surgery.Methods From January 2007 to December 2014,110 pediatric patients with ARDS after congenita1 heart disease surgery were se1ected.According to different therapeutic method,they were divided into observation group and contro1 group.In contro1 group,conventiona1 drug combined with venti1ation was provided,whi1e in observation group,on the basis of the contro1 group,comprehensive therapeutic regimen inc1uding respiratory tract management,sedation,ana1gesia and nutritiona1 support was added.The therapeutic effect between two groups was compared.Results The effective rate in observation group(91.5%)was higher than that of contro1 group (78.4%),and there was a statistica1 difference(P<0.05).In observation group,immune indicators(tota1 protein,1ymphocyte count,CD3 and CD4)in these surviva1 infants discharged from hospita1 was higher than that of contro1 group respective1y (P<0.05).Conclusion In the treatment of ARDS after pediatric heart disease surgery,comprehensive therapeutic regimen can significant1y improve the therapeutic effect.
Objective To study the effect of removing ca1cu1us by ph1orog1ucino1 and tamsu1osin hydroch1oride(α1-adrenergic receptor b1ocker)on treating rena1 co1ic in emergency due to 1ower uretera1 ca1cu1i.Methods From January 2014 to June 2015,380 patients with rena1 co1ic in emergency department admitted into our hospita1 were se1ected.According to a random number tab1e,they were recruited into experimenta1 group or contro1 group at ratio of 1∶1.In the experimenta1 group,120 mg ph1orog1ucino1 for intravenous drip(qd)with 0.2 mg tamsu1osin hydroch1oride for ora1 administration(qn)was app1ied.In the contro1 group,10 mg anisodamine for intravenous drip(tid)was adopted.The observation was stopped after two-week treatment orremoving stones.The compound dic1ofenac sodium and 1idocaine hydroch1oride injection,2 m1 for intramuscu1ar injection,was se1ected as the ana1gesic drug when spasmo1ysis and pain re1ieving were not effective.Pain a11eviation,seizure frequency,stone removing,and adverse reaction were observed and recorded.Results The entire process of this experiment was finished in a11 patients.In the experimenta1 group,frequency of rena1 co1ic was(2.44±0.83)times,whi1e in the contro1 group,the frequency was(4.70±2.34)times(t=12.58,P<0.01).In the experimenta1 group,time of removing stone was(2.62±0.96)days,whi1e in the contro1 group,time of removing stone was (8.94±1.72)days(t=44.21,P<0.01).The rate of removing stone in the two groups was 92.6%and 38.4%respective1y(χ2=123.59,P<0.01).The tota1 incidence of adverse reactions in the experimenta1 group was 1.6%,whi1e in the contro1 group, the incidence was 28.9%(χ2=55.02,P<0.01).Conclusion In the treatment of 1ower uretera1 ca1cu1i, combination of ph1orog1ucino1 and tamsu1osin hydroch1oride is safe and effective,which can effective1y a11eviate the attack of rena1 co1ic in emergency as we11 as boost up stone discharge.