Objective To investigate the mechanism and significance of microRNA-214 (miRNA-214) targeted regulation of HOXB7 to inhibit the invasion and metastasis of colorectal cancer (CRC) cells. Methods A total of 200 inpatients with CRC admitted to Yuebei People's Hospital Affiliated to Medical College of Shantou University from January 2018 to December 2020 were selected as the research subjects, and were divided into non-metastatic group (n=100)and metastatic group (n=100) according to the occurrence of metastasis, and 200 healthy individuals during the same period were selected as the control group. Molecular biological techniques were used to detect the expression of miRNA-214, HOXB7, and Wnt/β-catenin in tissue cells, and the clinical significance was analyzed. Results The expression level of miRNA-214 in the tissues of patients of non-metastasis group and metastasis group were lower than those of the control group, the differences were statistically significant (P<0.05). The expression level of miRNA-214 in CRC SW1116 and SW480 cell lines was lower than that in normal cells, the differences were statistically significant (P<0.05). Transfection of SW1116 and SW480 cells with miRNA-214 mimics showed that high expression of miRNA-214 resulted in low expression of HOXB7. HOXB7 was the target gene of miRNA-214, and the expression level of the two was negatively correlated (r=-0.394, P<0.05). The expression level of HOXB7 in non-metastatic group and metastatic group were higher than those in control group,and the differences were statistically significant (P<0.05). The expression level of HOXB7 in SW1116 and SW480 cell lines were higher than those in normal cells, and the differences were statistically significant (P<0.05). When HOXB7 was overexpressed, Wnt/β-catenin expression was also increased, while when HOXB7 was interfered with by miRNA-214, Wnt/β-catenin expression was significantly decreased. Conclusion The miRNA-214—HOXB7—Wnt/β-catenin pathway is involved in inhibiting the invasion and metastasis of colorectal cancer, which provides a new target for the diagnosis and treatment of colorectal cancer.

Objective Network pharmacology was used to analyze the effective active components and mechanism of Gynostemma pentaphyllum in the treatment of osteoporosis. Methods The effective active components and targets of Gynostemma pentaphyllum through the Traditional Chinese Medicine Systems pharmacology Database and Analysis Platform （TCMSP）. Targets related to osteoporosis were identified by GeneCards，Therapeutic Target Database （TTD）,Online Mendelian Inheritance in Man （OMIM） and Pharmacogenomics Knowledgebase （PharmGKB） databases. The intersection of drug targets and disease targets was analyzed by protein-protein interaction network analysis，GO function enrichment analysis and KEGG pathway analysis. Results Twenty-four effective active components of Gynostemma pentaphyllum, including quercetin, rhamnazin and isofucosterol, were obtained, corresponding to 217 targets. Through the GeneCards, TTD, OMIM and PharmGKB database, 4620 osteoporosis related targets and 121 intersection targets were obtained.The protein-protein interaction network analysis showed that JUN proto-oncogene （JUN）, tumor protein P53 （TP53）, AKT serine/threonine kinase 1 （AKT1）, mitogen-activated protein kinase 1 （MAPK1）, RELA protooncogene （RELA）, estrogen receptor 1 （ESR1）, mitogen-activated protein kinase 14 （MAPK14）, Fos proto-oncogene(FOS), Myc proto-oncogene （MYC）, signal transducer and activator of transcription 1 （STAT1） may be the core targets of the herb in the treatment of the disease.GO functional enrichment analysis showed 1964, 43 and 160 entries in biological process, cell composition and molecular function, respectively. KEGG pathway enrichment analysis revealed 162 signaling pathways involving lipids, atherosclerosis, chemical carcinogene-receptor activation, etc. Conclusion This study initially revealed that Gynostemma pentaphyllum can treat osteoporosis through multiple components, multiple targets and multiple pathways, and its molecular mechanism may be related to JUN, TP53,AKT1, MAPK1, RELA, ESR1, MAPK14, FOS, MYC, STAT1 and other targets. These pathways involve lipids and atherosclerosis, chemical carcinogenic receptor activation, fluid shear stress and atherosclerosis, which lay a foundation for further research.

 Objective To observe the clinical effect and safety of Pembrolizumab combined with Pemetrexed and Cisplatin (AP) chemotherapy in the treatment of advanced lung adenocarcinoma. Methods A total of 78 patients with locally advanced and advanced lung adenocarcinoma admitted to Department of Medical Oncology of Suzhou Hospital Affiliated to Nanjing Medical University from June 2018 to June 2020 were enrolled as the research objects retrospectively.According to different treatment methods, they were divided into AP chemotherapy group (n=37) and combination group(n=41). AP chemotherapy group was treated with AP chemotherapy (Pemetrexed 500 mg/m2 + Cisplatin 75 mg/m2),while combination group was treated with Pembrolizumab (200 mg) on basis of AP regimen. The curative effect, score of quality of life (QOL), progression-free survival (PFS), progression-free survival rate within 1 year and adverse reactions during treatment were compared between the two groups. Results The disease remission rate and disease control rate in combination group were higher than those in AP chemotherapy group, the differences were statistically significant (P<0.05). Improvement rate of quality of life in the combination group was higher than that in AP chemotherapy group, the difference was statistically significant (P<0.05). The progression-free survival rate within 1 year in combination group was higher than that in AP chemotherapy group, and PFS was longer than that in AP chemotherapy group, the differences were statistically significant (P<0.05). There was no significant difference in the incidence of adverse drug reactions between the two groups (P>0.05). Conclusion The short-term curative effect of Pembrolizumab combined with AP chemotherapy is more ideal, which can prolong survival time of patients to a certain extent, with few adverse reactions.And it provides new direction for clinical treatment of patients with advanced lung adenocarcinoma.

 Patients in intensive care unit are under acute stress due to their own diseases, the use of large doses of vasoactive drugs can easily lead to dysfunction of blood glucose regulation. The pain caused by various invasive catheters and invasive mechanical ventilation made the medical staff realize the importance of analgesic sedation. With the improvement of medical technology and concept, medical staff gradually pay attention to the nutritional supply, rehabilitation and psychological care of patients with severe diseases. As an important participant and implementer of medical and nursing programs, nurses play a vital role. At present, some studies have applied the nurse-led nursing model to the sedation and analgesia management, blood glucose regulation, early rehabilitation, psychological nursing and other aspects of critical patients, and achieved some results, this article will elaborate and summarize the above contents in detail, focusing on the medical responsibilities, clinical implementation methods, quality monitoring methods, effect evaluation standards, humanistic care and the advantages and disadvantages in the implementation of the scheme in the nurse-led nursing model, so as to provide a basis for clinical nursing work and a theoretical basis for subsequent related research.

 Anemia is a common complication of chronic kidney disease, which seriously affects the life span and quality of life of patients. Roxadustat, which is a oral small molecule hypoxia-inducible factor prolyl hydroxylase inhibitors,is first used in the treatment of renal anemia. It promotes the expression of erythropoietin and its receptors, inhibits hypoxia-inducible factor's ubiquitin degradation, maintains hypoxia-inducible factor's stability, improves iron absorption,utilization and transportation, comprehensively controls erythropoiesis. Its effect is distinct and it has become a new way to treat renal anemia. Recently, more and more evidences show that Roxadustat has more new applications. Among them, the mechanism of hypoxia protection has attracted much attention. Related animal studies have confirmed that Roxadustat can exert protective effect on kidney, lung, heart, muscle and bone tissues through anti-oxidative stress and anti-fibrosis. Meanwhile, related clinical trials have documented that roxadustat can effectively correct renal anemia in dialysis and non-dialysis patients, and is not affected by the inflammatory state. It is suitable for all age groups. So,this article reviews the progress of animal and clinical research in roxadustat at home and abroad, in order to provide a new basis for its application.

 Objective To investigate the correlation between fractional exhaled nitric oxide (FeNO) and interleukin-17(IL-17) and lung function in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).Methods A total of 80 patients with AECOPD admitted to Pingxiang People's Hospital from September 2020 to March 2021 were selected as the case group, and 60 healthy patients in the same period were selected as the control group.The levels of FeNO, IL-17 and lung function were measured in patients with AECOPD during the exacerbation period and the stable period, as well as in the control group. The correlation between FeNO, IL-17 and lung function (the first second forced expiratory volume [FEV1], the ratio of FEV1 to forced vital capacity [FVC] [FEV1/FVC]) was analyzed.Results The levels of FeNO and IL-17 in the COPD patients in the two periods were higher than those in the control group, and the differences were statistically significant (P<0.05). The levels of lung function (FEV1, FEV1/FVC) in the COPD patients in the two periods were lower than those in the control group, and the differences were statistically significant (P<0.05). There was no correlation between FeNO and IL-17, FEV1, FEV1/FVC in AECOPD patients (P＞0.05).There was a positive correlation between FeNO and IL-17 (r=0.236, P<0.05), and a negative correlation with lung function (FEV1, FEV1/FVC) (r=-0.178, -0.316, P<0.05). Conclusion FeNO has a certain correlation with IL-17 and FEV1,FEV1/FVC, and it can be used as an effective index for the evaluation of COPD.

Objective To investigate the relationship between peripheral T lymphocyte subgroup and complications in patients with scrub typhus. Methods A total of 53 patients with scrub typhus treated in Ruijin People's Hospital from January 2018 to September 2021 and 50 healthy people took physical examination in the same period were selected as the research objects.Then establish the observation group and the control group.Observation group 1 contains 53 patients with scrub typhus. Control group 1 contains 50 healthy people. Observation group 2 contains 37 patients with complications of scrub typhus (including 10 cases in single organ damage group, 14 cases in double organ damage group and 13 cases in multiple organ damage group). Control group 2 contains 16 patients without complications of scrub typhus.CD3+, CD4+, CD8+ T lymphocytes, CD4+/CD8+ ratio were observed before and after treatment in patients with/without complications and healthy people. Results The number of CD3+, CD4+, CD8+ T lymphocyte and CD4+/CD8+ ratio of observation group 1 were lower than those of control group 1,the differences were statistically significant(P<0.05).The above indicators of observation group 1 were higher than those before treatment, the differences were statistically significant(P<0.05). There were no significant differences in the above indexes between observation group 2 and control group 2 before treatment (P>0.05). There was no significant differences in the above indexes between the single organ damage group and the control group 2 (P>0.05). The number of CD3+T lymphocytes and CD4+/CD8+ ratio in the double organ damage group and multiple organ damage group were lower than those in the control group 2, the differences were statistically significant (P<0.05). The number of CD4+T lymphocytes in the double organ damage group was higher than that of control group,the difference was statistically significant (P>0.05). Conclusion T lymphocyte subgroup participates in the pathogenesis of scrub typhus, cellular immune dysfunction plays an important role in the pathogenesis of scrub typhus, and early diagnosis and treatment can better protect the cell immunity of scrub typhus patients, reduce the occurrence of complications and improve the prognosis.

Objective To explore the effect of electrical stimulation biofeedback combined with magnetic stimulation in the treatment of postmenopausal female pelvic organ prolapse. Methods A total of 100 postmenopausal female pelvic organ prolapse patients who were admitted to the Second People's Hospital of Guangdong Province from January 2019 to December 2020 were selected as the research objects, and they were divided into the observation group and the control group according to the random number table method, with 50 cases in each group. The control group was given electrical stimulation biofeedback for treatment, and the observation group was treated with magnetic stimulation on the basis of the control group. The treatment cycle of the two groups was 15 weeks. The treatment effect of the two groups were compared, and the grading of pelvic organ prolapse quantitative staging (POP-Q) and pelvic floor muscle strength of the two groups before and after treatment were compared. Results After 15 weeks of treatment, the total effective rate of the observation group was higher than that of the control group, the difference was statistically significant (P＜0.05).The grading of POP-Q of the two groups after 15 weeks of treatment was better than that before treatment, and the grading of POP-Q of the observation group after 15 weeks of treatment was better than that of the control group, the differences were statistically significant (P＜0.05). After 15 weeks of treatment, the pelvic floor muscle strength and the maximum contraction force of the pelvic floor muscle in the two groups were higher than those before treatment, and the continuous contraction time of the pelvic floor muscle in the two groups was longer than that before treatment, the differences were statistically significant (P＜0.05). After 15 weeks of treatment, the pelvic floor muscle strength and the maximum contraction force of the pelvic floor muscle in the observation group were higher than those in the control group, and the continuous contraction time of the pelvic floor muscle in the observation group was longer than that in the control group, the differences were statistically significant (P＜0.05). Conclusion Compared with electrical stimulation biofeedback therapy, electrical stimulation biofeedback combined with magnetic stimulation is more effective in the treatment of pelvic organ prolapse in postmenopausal women, which can effectively improve pelvic floor muscle strength and improve the quality of life of patients.

 Objective To explore the application effect of heat-sensitive moxibustion in patients with severe acute pancreatitis. Methods A total of 50 patients with severe acute pancreatitis in Ganzhou People's Hospital from June 2020 to July 2021 were selected as the research objects. They were divided into observation group and control group by computer-generated random distribution sequence, with 25 patients in each group. The patients in the observation group were treated with heat-sensitive moxibustion, and the patients in the control group were treated with routine symptomatic treatment. The incidence of gastrointestinal intolerance, the time of reaching the standard of enteral nutrition and the length of stay in ICU were compared between the two groups. Results The total incidence of gastrointestinal intolerance in observation group was lower than that in control group, and the difference was statistically significant(P<0.05). The time of reaching the standard of enteral nutrition and the length of stay in ICU in observation group were shorter than those in control group, the differences were statistically significant (P<0.05). Conclusion For patients with severe acute pancreatitis, the therapeutic effect of heat-sensitive moxibustion therapy is better, which can reduce the incidence of enteral nutritional intolerance, shorten the length of hospital stay, and restore the enteral nutritional function of patients. The scheme is worth recommending.

Objective To investigate the effect of modified ligation of the intersphincteric fistula tract (LIFT) on anal function of high complex anal fistula (CAF). Methods A total of 62 patients with high CAF treated in Yichun Hospital of Traditional Chinese Medicine in Jiangxi Province from February 2020 to January 2021 were selected as the research objects.They were divided into observation group(31 cases)and control group(31 cases)by random number table method.The patients in the observation group were treated with modified LIFT, and the patients in the control group were treated with incision and thread hanging. They were followed up for 8 months. The treatment effect, clinical related indexes,anal function, complications and recurrence of the two groups were compared. Results There was no significant difference in the total effective rate between the two groups (P>0.05). The operation time of the observation group was longer than that of the control group, the wound healing time and hospital stay were shorter than those of the control group,and the visual analogue scale (VAS) score and Wexner score were lower than those of the control group, with statistically significant differences (P<0.05). The total incidence rate and recurrence rate of complications in the observation group were lower than those in the control group, with statistically significant differences (P<0.05). Conclusion The modified LIFT can achieve the same effect as incision and thread hanging in the treatment of high CAF, and has more advantages in shortening the wound healing time, reducing pain and protecting anal function, and can reduce complications and disease recurrence.

 Objective To explore the effect of procedure for prolapse and hemorrhoids (PPH) in the treatment of severe hemorrhoids on the operation time of patients. Methods A total of 82 patients with severe hemorrhoids treated in Haikou Traditional Chinese Medicine Hospital of Hainan province from January 2020 to October 2021 were selected as the research objects, and they were divided into the control group and the research group by random number table method,with 41 cases in each group. The control group was treated with traditional surgery. And PPH was performed in the study group. The clinical treatment effect, clinical indicators and the incidence of complications were compared between the two groups. Results The total effective rate of the study group was higher than that of the control group, and the difference was statistically significant (P<0.05). The operative time, pain time, wound healing time and hospital stay in the study group were shorter than those in the control group, and the differences were statistically significant (P<0.05). The total incidence of complications in the study group was lower than that of the control group, and the difference was statistically significant (P<0.05). Conclusion PPH treatment can effectively optimize various clinical indicators, reduce the treatment time, reduce the incidence of complications, with high safety, good prognosis, significant effect, worthy of popularization and application.

Objective To explore the clinical efficacy of open liver tumor resection and laparoscopic liver tumor resection in treatment of liver tumors and their effect on liver function and inflammatory indexes. Methods A total of 60 patients with liver tumors treated in Qiandongnan Prefecture People's Hospital, Guizhou Province from March 2017 to August 2021 were selected as the research objects and divided into control group and experimental group by random number table method, with 30 cases in each group. The patients in the control group were treated with open liver tumor resection, and the patients in the experimental group were treated with laparoscopic liver tumor resection. The clinical indexes, liver function, inflammatory indexes and incidence of complications of the two groups were compared. Results The amount of intraoperative bleeding in the experimental group was less than that in the control group, and incidence of postoperative complications in the experimental group was lower than that in the control group, and the differences were statistically significant (P<0.05). The recovery time of gastrointestinal tract, extubation time of drainage tube and hospital stay in the experimental group were shorter than those in the control group, and the differences were statistically significant (P<0.05). The levels of aspartate aminotransferase, alanine aminotransferase, total bilirubin and C-reactive protein (CRP)in the experimental group were lower than those in the control group,and the differences were statistically significant(P<0.05).Conclusion Laparoscopic liver tumor resection has significant clinical efficacy,can significantly improve liver function and significantly reduce inflammatory reaction. It is a minimally invasive, effective and safe operation.

Objective To investigate effect of somatostatin on colorectal fistula after anorectal surgery. Methods A total of 64 patients with colorectal fistula treated in the Department of General Surgery of Maojian District People's Hospital of Shiyan City from September 2017 to September 2020 were selected as the research objects. They were divided into experimental group (32 cases) and routine group (32 cases) according to random number table method, the routine group was treated with basic treatment including fasting, infusion and anti-inflammatory, and the experimental group was treated with somatostatin. The recovery and clinical indexes of the two groups were compared. Results The time of normal diet, hospitalization and fistula closure in the experimental group were shorter than those in the routine group,and the differences were statistically significant (P<0.05). There was no significant difference in the total incidence of complications between the two groups (P>0.05). The levels of serum amylase (AMS) and bile acid in the experimental group were lower than those in the conventional group, the differences were statistically significant (P<0.05). There were no significant differences in the laboratory indexes between the two groups before treatment (P>0.05). After treatment, the hematocrit (HCT) in the experimental group was lower than that in the conventional group, the difference was statistically significant. Conclusion Somatostatin treatment can adjust serum, stabilize laboratory indexes and promote the repair of fistula in patients with colorectal fistula after anorectal surgery.

Objective To investigate the application effect of transcatheter arterial chemoembolization (TACE) combined with FOLFOX regimen continuous hepatic arterial infusion chemotherapy in patients with Barcelona stage B(BCLC-B) primary liver cancer (PHC). Methods Seventy patients with PHC stage BCLC-B admitted to Chaozhou Central Hospital from May 2018 to May 2021 were selected as the study subjects, and they were divided into experimental group (n=35) and control group (n=35) by random number table method. The experimental group was treated with continuous hepatic arterial infusion chemotherapy with TACE+FOLFOX regimen, and the control group was treated with TACE. The disease control rate, objective response rate, incidence of adverse reactions, and tumor marker levels before and after treatment were compared between the two groups. Results The objective response rate and disease control rate of the experimental group were higher than those of the control group, and the differences were statistically significant (P<0.05). After treatment, the levels of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate antigen (CA199) in the experimental group were lower than those in the control group, and the differences were statistically significant(P<0.05).There was no significant difference in the incidence of adverse reactions between the two groups(P>0.05). Conclusion Continuous hepatic arterial infusion chemotherapy with TACE combined with FOLFOX regimen has a good short-term effect on patients with BCLC-B PHC. The disease control effect of patients is significantly improved, and the level of serum tumor markers is reduced. The safety is tolerable, and it is worthy of further study.

Objective To investigate the mutation of BRAFV600E gene in patients with thyroid papillary carcinoma(PTC) with different clinical characteristics and the influencing factors of central cervical lymph node metastasis.Methods A total of 80 PTC patients admitted to the Department of Geriatrics, the Second Hospital of Lanzhou University from June 2019 to December 2021 were selected as the research subjects, samples were prepared to determine whether BRAFV600E gene mutation occurred. The BRAFV600E gene mutations in PTC patients with different clinicopathological characteristics were analyzed, and the relationship between central cervical lymph node metastasis and BRAFV600E gene mutations and related influencing factors were analyzed. Results Patients were divided into positive group (n=38) and negative group (n=42) according to whether BRAFV600E gene mutation occurred. The tumor size in the positive group was larger than that in the negative group, and the number of multifocal tumors, envelope invasion,central lymph node metastasis and unilateral lymph node metastasis in the positive group were higher than those in the negative group, the differences were statistically significant (P<0.05). According to whether the cervical lymph nodes in the central region metastasized, they were divided into metastasis group (n=20) and non metastasis group (n=9). The tumor size in the metastasis group was larger than that in the non metastasis group, and the incidence of bilateral tumors,multifocal tumors, capsule invasion and BRAF gene metastasis were higher than those in the non metastasis group, the differences were statistically significant (P<0.05). Multivariate logistic analysis showed that multifocal tumor, envelope invasion and BRAFV600E gene mutation were independent risk factors for cervical lymph node metastasis in the central region of PTC (P<0.05). Conclusion PTC tumor size, multifocal tumor, envelope invasion and BRAFV600E gene mutation can effectively predict central cervical lymph node metastasis. Preventive central lymph node dissection should be paid attention to in the formulation of surgical plan.

Objective To investigate the difference of survival time and its related factors in non-small cell lung cancer patients with brain metastases and negative driver gene treated by radiotherapy and gamma knife combined with radiotherapy. Methods A total of 170 patients with brain metastases from non-small cell lung cancer with negative driver gene were selected retrospectively as the research subjects from January 2018 to January 2021 in Qilu Hospital of Shandong University Eastern Court. According to different treatment methods, they were divided into radiotherapy group(135 cases) and gamma knife combined radiotherapy group (35 cases). The difference in survival and related influencing factors were compared between the two groups. Results There was no significant difference between the two groups in overall survival time (P>0.05). Cox regression analysis showed that Karnofsky score ≥70 points （β=-0.963, HR=0.382, 95%CI=0.205-0.710） and maximum lesion volume <10 ml （β=-0.567, HR=0.567, 95%CI=0.335-0.962） in the radio therapy group were protective factors for the overall survival of patients (P<0.05). In the gamma knife combined with radiotherapy group, the control of primary tumor （β=-2.414, HR=0.089, 95%CI=0.021-0.381） and the number of metastatic lesions less than 4 （β=-2.730, HR=0.065, 95%CI=0.013-0.323） were the protective factors for the overall survivnl of patients (P<0.05). Conclusion Non-small cell lung cancer patients and negative driver gene received radiotherapy alone or gamma knife combined with radiotherapy, and there was no difference in survival time between the two groups. When the primary focus is not effectively controlled or the number of metastatic focus was more than 4, the survival time of patients treated with radiotherapy alone was longer.

Objective To analyze the correlation between peak load of Epstein-Barr virus deoxyribonucleic acid (EBVDNA) and liver function in children with infectious mononucleosis induced by Epstein-Barr virus (EB virus). Methods A total of 60 children with EB virus-induced infectious mononucleosis who were admitted to Jiangxi Children's Hospital from January 2020 to December 2021 were selected as the observation group. Meanwhile, 60 children with common fever or respiratory tract infection were selected as the control group. Serum interleukin-6 (IL-6), EBV-DNA, CD3+and liver function indexes were compared between the two groups. Logistic regression model was used to analyze the influencing factors of infectious mononucleosis caused by EB virus. Pearson correlation analysis was used to analyze the correlation between EBV-DNA, IL-6, CD3+ and liver function. Results The levels of IL-6, EBV-DNA, serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total cholesterol (TC) in the observation group were higher than those in the control group, and CD3+ was lower than that in the control group, the differences were statistically significant (P<0.05). The results of logistic regression model showed that the increase of IL-6 (OR=1.063, 95%CI=2.899-1.673) and EBV-DNA (OR=1.079, 95%CI=2.983-1.257) was the risk factors of infectious mononucleosis caused by EB virus, and CD3+ (OR=0.512, 95%CI=0.036-0.646) was the protective factor of infectious mononucleosis caused by EB virus (P<0.05). Pearson correlation analysis showed that EBV-DNA and IL-6 were positively correlated with ALT, AST and TC (r＞0, P<0.05), and CD3+was negatively correlated with ALT, AST and TC (r<0, P<0.05). Conclusion CD3+, IL-6 and EBV-DNA are independent influencing factors of infectious mononucleosis caused by EB virus, and are closely related to liver function.

 Objective To analyze the application effect of gamma globulin combined with antibiotic de-escalation therapy in pediatric severe pneumonia. Methods A total of 96 children with severe pneumonia admitted to Ruijin People's Hospital of Jiangxi Province from June 2020 to June 2021 were selected as the study objects, they were divided into study group and reference group according to random number table method, with 48 cases in each group. The reference group was treated with the antibiotic de-escalation therapy based on the basic symptomatic treatment measures, the study group was treated with gamma globulin combined with antibiotic de-escalation therapy based on the basic symptomatic treatment measures. After 6 d of drug administration, the treatment effect of the two groups were observed and analyzed, and the changes of inflammatory factors and immune function such as hypersensitive C-reactive protein (hs-CRP), interleukin-6 (IL-6), immunoglobulin A (IgA) and immunoglobulin G (IgG) were measured in the two groups,and the total incidence of adverse reactions was counted and compared between the two groups. Results The total effective rate of the study group was higher than that of the reference group, the difference was statistically significant (P<0.05). There were no significant differences in hs-CRP, IL-6, IgA and IgG between two groups before treatment (P>0.05). After treatment, the hs-CRP and IL-6 of the two groups were lower than those before treatment, while IgA and IgG were higher than those before treatment, the differences were statistically significant (P<0.05). The hs-CRP and IL-6 of study group were lower than those of the reference group, IgA and IgG were higher than those of the reference group, the differences were statistically significant (P<0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P>0.05). Conclusion The treatment of severe pneumonia in children with gamma globulin combined with antibiotic de-escalation therapy can improve the immune function of the body, improve the clinical efficacy, and promote the improvement of inflammatory factors. It has good safety and application effect.

Objective To study the effect of different resection methods of hysteroscopic electroresection on the incidence of intrauterine adhesion and pregnancy outcome in patients with type Ⅱsubmucosal myoma of uterus. Methods A total of 78 patients with type Ⅱsubmucosal myoma of uterus who underwent hysteroscopic surgery at Yangjiang Maternal and Child Health Care Hospital from October 2017 to October 2020 were selected as research objects, they were divided into experimental group (n=39) and control group (n=39) according to random number table method. The experimental group used the resection method after opening the window, and the control group used the direct resection method. The perioperative indicators, follow-up indicators and pregnancy outcome were compared between the two groups. Results There were no statistically significant differences in the operation duration, intraoperative blood loss and uterine dilatation fluid dosage between the two groups (P>0.05). There was no significant difference in the recurrence rate of the two groups within 1 year after operation (P>0.05). Within 1 year after the operation, the difference in the healing rate of the myometrium between the two groups was not statistically significant (P>0.05). At the 1st and 6th months after the operation, the endometrial wound healing rate in the experimental group was higher than that in the control group, and the difference was statistically significant (P＜0.05). Within 1 year after surgery, the incidence of intrauterine adhesions in the experimental group was lower than that in the control group, the difference was statistically significant (P<0.05). Within 1 year after operation, the success rate of pregnancy in the experimental group was higher than that in the control group, and the difference was statistically significant (P<0.05). Conclusion Hysteroscopic electroresection after opening the window should be used in the treatment of type Ⅱsubmucosal myoma of uterus. It can promote the rapid healing of endometrial wound, prevent postoperative intrauterine adhesion, and improve the success rate of pregnancy. It has no effect on perioperative indicators, myometrial healing and postoperative recurrence rate of patients. It is worth popularizing.

 Objective To investigate the application effect of continuous penetrating suture of nasal septum in patients with sinusitis and nasal polyp with nasal septum deviation. Methods A total of 90 patients with chronic sinusitis and nasal polyps accompanied by nasal septum deviation admitted to Shangrao People's Hospital from January to June 2021 were selected as the research subjects, and they were divided into the observation group (45 cases) and the control group (45 cases) according to the random number table method. The control group was treated with nasal packing after functional nasal endoscopy and nasal septum correction, and the observation group was treated with continuous penetrating suture of nasal septum after functional nasal endoscopy and nasal septum correction. The clinical symptom scores and complications were compared between the two groups. Results The scores of headache, nasal pain, toothache and lacrimal discharge in the two groups after operation were lower than those before operation, the scores of headache,nasal pain, toothache and tears in the observation group after operation were lower than those in the control group, the differences were statistically significant (P＜0.05). The total incidence rate of complication of the observation group was lower than that of the control group, and the difference was statistically significant (P＜0.05). Conclusion Patients with sinusitis and nasal polyps accompanied by nasal septum deviation are treated with continuous penetrating suture of nasal septum, which can improve postoperative headache, nasal pain, toothache, lacrimal discharge and other problems,and reduce complications.

Objective To investigate the clinical effect of Spleen Aminopeptide Oral Solution (SAOS) in the prophylactic treatment of seasonal allergic rhinitis (AR) and its effect on immune function. Methods A total of 52 patients with seasonal AR who were treated in the Affiliated Hospital of Inner Mongolia Medical University from May 2015 to May 2019 were selected as the research objects, and they were divided into group A (n=20), group B (n=20) and group C(n=12) according to the random number table method. Patients in group A received SAOS alone, group B received both SAOS and Loratadine, group C received Loratadine alone. The three groups were given different preventive intervention before the onset of AR. The influence of different drugs on the symptoms, quality of life, combined medication and T helper lymphocyte (Th) subsets (Th1/Th2) cytokines of patients with seasonal AR were compared among the three groups. Results At 2 weeks of treatment, the total score and sub item score of rhino-conjunctivitis quality of life questionnaire (RQLQ) in group B were lower than those in group C, the differences were statistically significant (P＜0.05). At 4 weeks of treatment, the total symptom score of group B was lower than that of group C, the difference was statistically significant (P＜0.05). The total score and sub item score of RQLQ in group B were lower than those of group C, the differences were statistically significant (P＜0.05). At 12 weeks of treatment, the scores of nasal symptoms and emotional reactions of group B were lower than those of group C, and the nasal symptoms score of group A was lower than those of group C, the differences were statistically significant (P＜0.05). In the pollen peak period (4 and 8 weeks of medication), the score of combined medication of group B was lower than that of group A and group C, the differences were statistically significant (P＜0.05). Cytokine detection of Th1/Th2 showed that the levels of interleukin (IL)-2, interferon-γ (IFN-γ), IL-4, IL-10 in group A after treatment were higher than those before treatment, and the differences were statistically significant (P＜0.05). The levels of IL-2, IFN-γ, tumor necrosis factor-α(TNF-α), IL-4, IL-6, IL-10 in group B after treatment were higher than those before treatment, the differences were statistically significant (P＜0.05). The levels of IL-2, IFN-γ, IL-4, IL-10 in group C after treatment were higher than those before treatment, the differences were statistically significant (P＜0.05). There were no significant differences in the levels of cytokines among the three groups after treatment (P＞0.05). Conclusion The combination of SAOS and Loratadine in the prophylactic treatment of seasonal AR can effectively relieve the symptoms, improve the quality of life,and affect the expression of Th1/Th2 cytokine levels, the curative effect is remarkable.

Objective To study the application effect of periodontal foundation therapy in patients with chronic periodontitis and type 2 diabetes mellitus. Methods From March 2020 to 2021, 80 patients with chronic periodontitis complicated with type 2 diabetes mellitus treated into Sanming Integrated Medicine Hospital from March 2020 to March 2021 were selected as the research objects. They were divided into the control group (40 cases) and the intervention group (40 cases) according to ballot methods. The control group was treated with Ornidazole Tablets and Cefaclor, while the intervention group was treated with periodontal basic therapy on the basis of the control group. The periodontal treatment indexes (sulcus bleeding index [SBI] and periodontal probing depth [PD]) and the inflammatory factor index(tumor necrosis factor-α [TNF-α] and high sensitive C-reactive protein [hs-CRP]) and blood glucose index (fasting blood glucose and 2-hour postprandial blood glucose level) were compared between the two groups. Results The levels of SBI and PD in the intervention group were lower than those in the control group, the differences were statistically significant (P＜0.05). The levels of TNF-α and hs-CRP in the intervention group were lower than those in the control group, the differences were statistically significant (P＜0.05). The fasting blood glucose level and 2-hour postprandial blood glucose level in the intervention group were lower than those in the control group, the differences were statistically significant (P＜0.05). Conclusion The application effect of periodontal basic therapy is effective in patients with chronic periodontitis and type 2 diabetes mellitus. It can effectively relieve and eliminate periodontal diseases, reduce inflammatory factors, and reduce blood glucose level, promote blood glucose control, improve physical and mental health and quality of life, and is worthy of popularization and application.

 Objective To observe the application effect of Sevoflurane inhalation by combined inhalation anesthesia induction device in reduction developmental dysplasia of the hip (DDH) in children. Methods A total of 97 children with the diagnosis of DDH were selected from the People's Liberation Army Ground Force Seventy-three Military Hospital,Chenggong Hospital affiliated to Xiamen University from January 2016 to January 2020, and divided into group G (n=49)and group D (n=48)by random number table method.The group G was inhaled Sevoflurane 2 ml once by using combined inhalation anesthesia induction device before manual reduction with the supplementary dose of Sevoflurane 2 ml once, and group D was injected Ketamine 4-5 mg/kg through deltoid muscle with the supplementary dose of 0.5 mg/kg intravenously. The mean arterial pressure (MAP), heart rate (HR), blood oxygen saturation (SpO2), sleeping latency, wakening time, and the total incidence of adverse events were collected and analyzed at pre-anesthesia (T1), pre-operation(T2), and post-operation (T3). Results There were no significant differences in MAP, HR and SpO2 between the two groups at T1 (P＞0.05). The fluctuation of MAP, HR in group G at T2 and T3 were lower than those in group D, with statistically differences (P＜0.05). There were no significant differences in SpO2 at T2 and T3 between group G and group D(P＞0.05). The fluctuation of MAP, HR in group Gg at T2 and T3 were lower than those in group Dd in children of who required manual reduction after adductor amputation,with statistically differences (P<0.05).There were no significant differences in SpO2 at T2 and T3 between group Gg and group Dd (P＞0.05). The sleep latency in group G was longer than that in group D,with statistically difference(P<0.05).The awakening time was shorter than that in group D, with statistically difference (P<0.05). The total incidence of adverse events in group G was lower than that in group D, with statistically difference (P<0.05). Conclusion Compared with traditional anesthesia applied by ketamine, the inhalation of Sevoflurane by combined inhalation anesthesia induction device during manual reduction of DDH in children were more humanization and safety, and with the advantages of stable anesthesia, rapid recovery and less incidence of adverse events. It is a safe and effective anesthesia method.

Objective To analyze the effect of ultrasound-guided nerve block combined with general anesthesia on analgesia and hemodynamics in patients with total knee replacement. Methods A total of 80 patients with total knee replacement treated in Longyan First Hospital Affiliated to Fujian Medical University from January 2019 to June 2021 were selected as the research objects. They were divided into control group (n=40) and observation group (n=40) by random number table method. The control group were treated with general anesthesia, and the observation group were treated with ultrasound-guided lumbar plexus sciatic nerve block combined with general anesthesia. The visual analogue scale (VAS) score, analgesic situation, anesthetic dosage and hemodynamic changes were compared between the two groups. Results The heart rate and mean artery pressure of the observation group during intubation and extubation were higher than those of the control group, the differences were statistically significant (P<0.05). The VAS score of the observation group was lower than that of the control group, the time from the first demand for patient-controlled analgesia pump (PCA) to conscious extubation was longer than that of the control group, the recovery time of anesthesia was shorter than that of the control group, the pressing times of analgesia pump and the use of Sufentanil within 24 hours after operation were less than those of the control group, and the score of patients' satisfaction with analgesia was higher than that of the control group, the differences were statistically significant (P<0.05). The doses of Propofol, Remifentanil and Sufentanil in the observation group were lower than those in the control group, the differences were statistically significant (P<0.05). There was no significant difference in anesthesia time between the two groups (P＞0.05). The recovery time and extubation time of the observation group were shorter than those of the control group, the differences were statistically significcant (P<0.05). Conclusion Using ultrasound-guided nerve block combined with general anesthesia can maintain stable hemodynamics and have good analgesic effect. It can effectively reduce patients' pain and reduce the dosage of narcotic analgesics. It has high application value.

Objective To understand the internship satisfaction and professional identity of dental interns, analyze the differences in their internship status and professional identity, understand the problems existing in the internship, and explore the factors affecting internship satisfaction and the relationship between internship satisfaction and professional identity. In order to improve the practice environment, improve the quality of practice teaching, and improve the sense of professional identity, it puts forward reference suggestions and promotes the training of dental talents. Methods From September 2020 to June 2021, 112 interns in Hangzhou from Zhejiang University of Traditional Chinese Medicine majoring in stomatology were selected as the research objects, and the questionnaires were distributed through the internet on their internship satisfaction and professional identity. A survey was conducted to explore the influencing factors of internship satisfaction and the correlation between internship satisfaction and professional identity. Results In this survey, the total score of internship satisfaction of interns was (50.45±7.09) points, and the item average score was (3.88±0.55) points. The total score of professional identity was (53.61±7.90) points, and the item average score was (4.12±0.61) points. The internship satisfaction of the public unit was higher than that of the private unit, and the difference was statistically significant (P<0.001). There were no statistically significant differences in the internship experience with different gender, educational background, origin of students, the selection method of the internship unit (P>0.05).Internship satisfaction was positively correlated with their professional identity (r=0.587, P<0.01). Conclusion The internship satisfaction and professional identity of the interns are in the middle and high level, but there is still room for improvement. The nature of the internship unit has an impact on the internship satisfaction of interns, and the internship satisfaction has a positive moderating effect on the formation and consolidation of professional identity.

Objective To evaluate the quality of life of patients with advanced schistosomiasis in the Chaisang District and to analyze the influencing factors, so as to provide a basis for improving the quality of life of patients with advanced schistosomiasis. Methods A total of 216 patients with advanced schistosomiasis who were hospitalized in the Department of Diagnosis and Treatment of Schistosomiasis Clinic of the Chaisan District Center for Disease Control and Prevention in Jiujiang City from January 2020 to June 2021 were selected as the case group, and 216 healthy residents without schistosomiasis at the same time were selected as the control group. The World Health Organization quality of life assessment instrument brief version (WHOQOL-BREF) was used to investigate the factors influencing the survival quality of patients with advanced schistosomiasis by comparing the two groups. Results The quality of life and health status of the case group were worse than those of the control group, and the differences were statistically significant (P<0.05). The scores of physiological and psychological fields in the case group were lower than those in the control group,and the differences were statistically significant (P<0.05), while there were no significant differences in the scores of social relations and environment between the two groups (P>0.05). The results of univariate analysis suggested that there were significant differences in the comprehensive level hierarchical composition of quality of life between advanced schistosomiasis patients with different sleep quality, marital status, number of exercise activities in weeks, presence or absence of comorbidities, per capita household income, dietary status, and family discordance advanced schistosomiasis(P<0.05). The results of multivariate analysis suggested that age (β=-1.605, OR=0.201, 95%CI=0.096-0.422), sleep quality (β=1.169, OR=3.220, 95%CI=1.459-7.104), number of exercise activities per week (≥30 min/session) (β=1.563, OR=4.772, 95%CI=2.275-10.010), per capita household income (β=0.994, OR=2.702, 95%CI=1.350-5.406), with or without comorbidities (β=-0.737, OR=0.478, 95%CI=0.235-0.973), appetite (β=0.947, OR=2.578, 95%CI=1.284-5.177) and family discordance (β=-1.019, OR=0.361,95%CI=0.177-0.737) were found to affect the quality of life of patients with advanced schistosomiasis (P<0.05). Conclusion The quality of life of patients with advanced schistosomiasis is lower than that of healthy residents without schistosomiasis, and age, sleep quality, number of exercise activities in weeks, monthly household income, presence or absence of comorbidities, appetite and family discordance are the main factors influencing the quality of life of patients with advanced schistosomiasis.

Objective To analyze the infection of Ureaplasma urealyticum (UU), Neisseria gonorrhoeae (NG) and Chlamydia trachomatis (CT) in order to provide effective scientific basis for epidemic investigation. Methods A total of 12 991 female patients who visited the outpatient Beijing Chaoyang Maternal and Child Hospital and underwent physical examination from January 2013 to December 2018 were selected as subjects. All subjects were tested for UU, NG and CT by diversion hybridization technique, and the infection of three pathogens in female reproductive tract was observed. Results The positive rates of UU, CT and NG in 12 991 females were 61.63%, 9.67% and 0.62%, respectively.The main infection type was UU alone, followed by CT alone and UU+CT mixed infection, accounting for 54.69%,2.93% and 6.49%, respectively. Among 12 991 women, 8407 were positive, with a total positive rate of 64.71%. The highest positive rate was 72.27% in age > 15-20 years old, and the lowest positive rate was 45.13% in age over 50 years old. In 2013, the total positive rate and the rates of UU, CT, NG and mix infecction were the lowest. The positive rates of UU and CT in different departments were similar, only the positive rate of NG in department of obstetric was 0.Conclusion UU, CT, and NG are important pathogens of reproductive tract infections in Beijing Chaoyang Maternal &Child Hospital's outpatient department. Ureaplasma urealyticum is the main pathogen of infections. The positive rate is increasing year by year. The positive rate is the highest for people under 20 years old. It is necessary to strengthen surveillance and be targeted general sieve. Women of childbearing age should strengthen the screening of pre-pregnancy physical examination.

Under the background of Healthy China Initiative and Three-Child policy, the healthy growth of children has received more and more important attention in China. Integrate the high-quality development of children's health into the overall economic and social development has become the consensus of the Child-friendly city construction.And the Child-friendly hospitals play a very important role in it. But at present, the construction of the Child-friendly hospitals in China is still in its infancy.This article takes the Children's Hospital of Soochow University as an example,and focuses on its advantages in children's specialty, continuous improvement of medical services, and protection of regional children's health. The author hopes to introduce the hospital environment, medical services, health management,charity social work, etc. management experience, analyzing the hospital's ideas and plans for building the Child-friendly hospital, thinking about the long-term path of the Child-friendly hospital construction.

Since the reform of Drug Regulatory System in 2015 as well as the Drug Review and Approval System, the level of domestic drug clinical trials has promoted rapidly and China's pharmaceutical innovation industry has been continuously integrated with the chain of global innovation industry. As the import of global innovative drugs being promoted, domestic new drugs are also gradually going to the world. The newly revised Drug Administration Law (2019)and Drug Registration Administration Measures (2020) have further stimulated the innovative drugs research and development with drug clinical trials in China, which becomes the major development trend. However, an increasing number of drug clinical trials are involved in the collection, utilization, export and etc. of human genetic resources. Therefore, strengthening the management of human genetic resources is vital to national security. In this paper, analyzed the current situation of human genetic resources management during the whole process including the beginning, ongoing and ending of drug clinical trials in Sir Run Run Hospital, Nanjing Medical University. The corresponding suggestions covering the division of responsibility, organization and training, quality management system and the information management process of human genetic resources management, in order to further improve the quality of drug clinical trials.

Objective To explore a convenient and efficient cleaning and disinfection method for Da Vinci robotic surgical arm. Methods A total of 100 Da Vinci robotic surgical arms recovered from the operation room of Jiangxi Provincial People's Hospital from December 2020 to September 2021 were selected and divided into experimental group and control group according to different operation procedures, with 50 Da Vinci robotic surgical arms in each group. In the experimental group, the operating end of the surgical arm was cleaned by running water washing+laparoscopic enzyme immersion+ultrasonic cleaning machine+steam cleaning machine+pure water rinsing with high-pressure water gun +75% alcohol disinfection+drying cabinet. The cleaning and disinfection process of the control group was as follows:flowing water washing+laparoscopic enzyme immersion+ultrasonic cleaning machine ultrasound+scrub operating end of surgical arm+high-pressure water gun rinse with pure water +75% alcohol disinfection+drying cabinet; Visual inspection method and ATP bioluminescence rapid detection method were used to detect the qualified rate of cleaning and disinfection, and the cleaning and disinfection effect and average cleaning operation time of the two groups were compared.Results The qualified rate of cleaning and disinfection in the experimental group was higher than that in the control group, and the difference was statistically significant (P<0.05). The qualified rate of cleaning and disinfection in the experimental group using ATP bioluminescence rapid detection was higher than that in the control group, and the difference was statistically significant(P<0.05).The average cleaning and disinfection time of the experimental group was shorter than that of the control group,and the difference was statistically significant(P<0.05).Conclusion The effect of cleaning and disinfecting Da Vinci robotic surgical arm by steam cleaning machine is more ideal than that by manual cleaning and disinfecting, which effectively saves rare medical resources and a lot of cleaning and disinfecting time.

 Objective To explore the application effect of multiple decontamination combined cleaning methods in the non-detachable orthopedic hollow electric drill, in order to find a correct and effective cleaning method. Methods A total of 120 pieces of non-detachable orthopedic hollow electric drills used in orthopedic surgery in Sichuan Forestry Central Hospital from March 2019 to May 2021 were randomly and equally divided into traditional group and improved group, with 60 cases in each group. The traditional group used wiping cleaning method to clean all parts of the main engine, drill chuck without soaking, brushing or washing, and cavity channel without brushing or washing. In the improved group, multiple decontamination combined cleaning methods were adopted, multi-enzyme solution pre-soaking drill chuck+professional brush brushing+high pressure water gun washing+wiping cleaning. The cleaning quality was compared by visual inspection and occult blood test. Results The qualified rates of visual inspection and occult blood test after cleaning in the improved group were higher than those in the traditional group, and the differences were statistically significant (P<0.05). Conclusion The correct and effective combined cleaning method of multiple decontamination can significantly improve the cleaning quality of non-detachable orthopedic hollow electric drill.

 Objective To explore the application effect of online and offline training courses for family caregivers in elderly family caregivers. Methods The convenience sampling method was adopted to select 101 trainees from two classes of a vocational qualification training course for primary family caregivers in Binjiang District, Hangzhou in March 2021 as the research objects, 51 trainees from one class as the experimental group, and 50 trainees from the other class as the control group. The experimental group adopted the mixed teaching mode, while the control group adopted the traditional teaching mode. The knowledge questionnaire scores and course satisfaction ratings of family caregivers were compared between the two groups. Results The scores of life care, basic care and rehabilitation service of the trained family caregivers in the experimental group were higher than those in the control group, the differences were statistically significant (P<0.05). The satisfaction of experimental group was better than that of control group, and the difference was statistically significant (P<0.05). Conclusion Compared with traditional teaching mode, blended teaching mode can effectively improve theoretical knowledge and satisfaction of family caregivers.

Objective To explore the feasibility of the application of standardized patient-based psychiatric nursing micro-lectures in nursing student internships. Methods A total of 108 undergraduate nursing students from Fujian Medical University, Fujian University of Traditional Chinese Medicine and Xiamen Medical College who were practicing in the Department of Psychiatry, Xiamen Xianyue Hospital from October 2020 to May 2021 were selected as the research subjects. And they were divided into experimental group and control group by random number table method, with 54 students in each group. The control group adopted the traditional clinical practice teaching method, and the experimental group adopted the standardized patient-based psychiatric nursing micro class teaching method on the basis of the traditional clinical practice teaching method. The results of theory test, operation test and teaching satisfaction were compared between the two groups at the end of clinical practice. Results At the end of clinical practice, the theoretical test scores and operation test scores of the experimental group were higher than those of the control group, and the differences were statistically significant (P<0.05). The scores of teaching satisfaction in experimental group were higher than those in control group, and the differences were statistically significant (P<0.05). Conclusions Standardized patient-based psychiatric nursing micro class teaching method can effectively improve the quality of nursing teaching and nursing students' satisfaction with the overall teaching process of clinical nursing practice.

Objective To explore the teaching effect of lecture-based learning (LBL) and case-based learning (CBL)combined with new technology mode. Methods A total of 128 clinical medical students who were interned and rotated in the Department of Orthopedics of the Second Affiliated Hospital of Xi'an Jiaotong University from early May 2020 to the end of July 2020 were selected as research objects. They were divided into LBL group and CBL group according to random number table method, with 64 cases in each group. LBL group conducted LBL teaching mode, while CBL group conducted CBL combined with new technology teaching mode, then quantitatively compare the results of the two groups and the feedback information of students through examination and questionnaire. Results The results of written exammination and operational examination in CBL group were better than those in LBL group, the differences were statistically significant (P<0.05). In terms of students' feedback, CBL group had higher feedback evaluation on learning interest, clinical thinking construction and team cooperation ability than LBL group, the differences were statistically significant (P<0.05). There were no significant differences in the mastery of clinical knowledge and doctor-patient communication ability between the two groups (P>0.05). Conclusion CBL combined with new technology teaching mode can significantly improve the teaching quality during practice rotation, improve students' learning interest, clinical thinking construction and teamwork ability. This research may provide guidance for clinical teaching.

Iron deficiency anemia is clinically common in chronic bleeding resulting in depletion of iron storage in the body, followed by iron deficiency in red blood cells, resulting in iron deficiency anemia, its incidence in women of childbearing age significantly increased, iron deficiency anemia treatment principle is to actively seek causes and make up iron storage, correct anemia. Globin-producing aplastic anemia, formerly known as thalassemia, is a group of hereditary hemolytic anemia diseases. An anemia or pathological condition resulting from an inherited genetic defect that results in the absence or insufficiency of the synthesis of one or more globin chains of hemoglobin. The disease is widely distributed in many areas of the world, southeast Asia is one of the high incidence areas. Sporadic cases occur in Guangdong, Guangxi and Sichuan, and sporadic cases occur in provinces and regions south of the Yangtze River, but rarely in the north. Clinical diagnosis of iron deficiency anemia, thalassemia is often not further screening, resulting in the occurrence of genetic diseases. This paper reported a case of thalassemia masked by iron deficiency anemia, suggesting that thalassemia screening can be improved in the accurate diagnosis of iron deficiency anemia, and it is of great significance for the diagnosis and treatment of anemia and eugenics.

 Posterior reversible encephalopathy syndrome (PRES) is a rare acute neurological disease with complex etiology and reversibility. It can be completely recovered after timely treatment. However, its clinical manifestations and imaging examination are lack of specificity, which is easy to be misdiagnosed and missed, affecting treatment and prognosis. Henoch-Schonlein purpura (HSP) is the most common rheumatic immune system disease in children, and reports of PRES are increasing. In order to improve pediatricians' understanding of HSP complicated with PRES in children, this paper retrospectively analyzed the clinical and imaging data, treatment and prognosis of two children with HSP complicated with PRES, and combined with literature review, emphasized that if convulsion, headache, visual impairment or consciousness disturbance occurred during the treatment of HSP children. Attention should be paid to the possibility of concomitant PRES, and early diagnosis and treatment of HSP-related PRES should be made to improve the prognosis of children.

Objective To explore the effect of humanistic care combined with predictive nursing on the cognitive function and negative emotions of patients with epilepsy. Methods A total of 120 epilepsy patients admitted to Guangdong 999 Brain Hospital from July 2020 to May 2021 were selected as the research objects, and were divided into control group (60 cases) and observation group (60 cases) according to the random number table method. Both groups of patients were treated with conventional epilepsy treatment. The control group received routine nursing care,while the observation group received humanistic care combined with predictive nursing on the basis of the control group. The cognitive function, anxiety and depression scores of the two groups of patients before and after the intervention were compared. Results There were no significant differences in the cognitive function, anxiety and depression scores of patients in the two groups before the intervention (P>0.05). The cognitive function scores of patients in the two groups after the intervention were higher than those before the intervention, and the scores of anxiety and depression were both lower than those before the intervention, the differences were statistically significant(P<0.05). The cognitive function score of observation group after intervention was higher than that of the control group,and the anxiety and depression scores were lower than those of the control group, the differences were statistically significant (P<0.05). Conclusion Humanistic care combined with predictive nursing can improve the cognitive function of epilepsy patients and reduce their negative emotions.

Objective To explore the application effect of phased continuous nursing in self-care ability of arteriovenous fistula in hemodialysis patients. Methods Fifty-six hemodialysis patients from Yingtan People's Hospital from January 2020 to June 2021 were selected as the research objects, and they were divided into observation group and control group by computer random allocation method, with 28 cases in each group. Patients in the observation group adopted staged continuous nursing care model, while patients in the control group adopted conventional nursing care.The knowledge of self-care of internal fistula, total nursing satisfaction, and self-care ability scores of the two groups were compared. Results The knowledge of self-care of internal fistula in the observation group was higher than that in the control group, and the difference was statistically significant (P<0.05). The total nursing satisfaction of the observation group was higher than that of the control group, and the difference was statistically significant (P<0.05). The scores of health knowledge level, self-concept, self-care responsibility, self-care skills in the observation group were higher than those in the control group 3 months after discharge, the differences were statistically significant (P<0.05). Conclusion Phased continuous nursing for hemodialysis patients can significantly improve the self-care ability of arteriovenous fistula and improve the relationship between nurse and patient, which is worthy of research and promotion.

Objective To explore the application effect of mobile APP in the rehabilitation nursing of elderly patients with hip arthroplasty. Methods From June 2020 to June 2021, 60 elderly patients who underwent hip arthroplasty in the Department of Orthopaedic Surgery of the Fifth Affiliated Hospital (Zhuhai) of Zunyi Medical University were selected as the research objects. The patients were divided into the intervention group (n=30) and the control group (n=30) according to random number table method. The control group was given routine nursing during the perioperative nursing of minimally invasive total hip arthroplasty. In addition to receiving perioperative routine nursing of hip replacement, the intervention group learned about the patients' rehabilitation through mobile APP and provided rehabilitation exercise guidance according to the patients' own conditions, so as to enhance the patients' confidence in rehabilitation. The hip function recovery, activity recovery, quality of life, complications and compliance with rehabilitation exercise were compared between the two groups. Results There were no significant differences in the Harris score,Barthel index and quality of life score between the two groups before intervention (P＞0.05). After treatment, the Harris scores, Barthel index scores and quality of life scores of the two groups were higher than those before treatment, the differences were statistically significant (P＜0.05). The Harris score, Barthel index and quality of life score of the observation group after treatment were higher than those of the control group, the differences were statistically significant (P＜0.05). The total incidence of complications in the observation group was lower than that in the control group, and the difference was statistically significant (P＜0.05). The total compliance rate of the observation group was higher than that of the control group, and the difference was statistically significant (P＜0.05). Conclusion Mobile APP intervention has a good postoperative nursing effect on elderly patients with hip arthroplasty, it can promote the recovery of patients' hip function, which is worth promoting.

Objective To explore the application effect of comfort nursing in inpatients in gastroenterology department.Methods A total of 82 inpatients in the Department of Gastroenterology of the Second People's Hospital of Yichun City from January to September 2021 were analyzed retrospectively. They were divided into observation group (41 cases) and control group (41 cases) according to different nursing methods. The control group received routine nursing, while the observation group received comfort nursing on the basis of the control group. The quality of sleep, quality of life and comfort were compared between the two groups. Results The sleep quality score of the observation group was lower than that of the control group, with statistical significance (P<0.05). After intervention, the scores of psychology, society,environment and physiology in QOL in the observation group were higher than those in the control group, with statistical significances (P<0.05). After intervention, the scores of physiology, psychology, spirit, social culture and environment in general comfort questionnaire (GCQ) in the observation group were higher than those in the control group, with statistical significances (P<0.05). Conclusion Comfort nursing has a good application effect in inpatients in gastroenterology department, which is conducive to improving comfort, sleep quality and quality of life.

Objective To explore the application value of 360° health education in the perioperative period of elderly patients with cataract. Methods A total of 88 patients undergoing senile cataract surgery admitted to Qianjiang Central Hospital from June 2020 to October 2020 were selected as the subjects, and they were divided into the conventional group (44 cases) and the study group (44 cases) using a random number table method. The conventional group used general perioperative nusing, and the study group was given 360° health education on the basis of the conventional group. The time from admission to discharge, the grasp degree of health education knowledge, the degree of nursing satisfaction and postoperative visual acuity recovery were compared between the two groups. Results The time from admission to discharge in the study group was shorter than that in the conventional group, and the difference was statistically significant (P＜0.05). The grasp degree of knowledge of cataract health education in the study group was higher than that in the conventional group, and the difference was statistically significant (P＜0.05). The total satisfaction of nursing in the study group was higher than that of the conventional group, and the difference was statistically significant (P＜0.05). The postoperative visual acuity recovery of patients in the study group was better than that of the conventional group, and the difference was statistically significant (P＜0.05). Conclusion The use of 360° health education in the perioperative period of elderly patients with cataract can effectively improve the grasp of health knowledge of elderly patients and nursing satisfaction, and have better postoperative vision recovery, which is worthy of promotion.