Objective To understand the internship satisfaction and professional identity of dental interns, analyze the differences in their internship status and professional identity, understand the problems existing in the internship, and explore the factors affecting internship satisfaction and the relationship between internship satisfaction and professional identity. In order to improve the practice environment, improve the quality of practice teaching, and improve the sense of professional identity, it puts forward reference suggestions and promotes the training of dental talents. Methods From September 2020 to June 2021, 112 interns in Hangzhou from Zhejiang University of Traditional Chinese Medicine majoring in stomatology were selected as the research objects, and the questionnaires were distributed through the internet on their internship satisfaction and professional identity. A survey was conducted to explore the influencing factors of internship satisfaction and the correlation between internship satisfaction and professional identity. Results In this survey, the total score of internship satisfaction of interns was (50.45±7.09) points, and the item average score was (3.88±0.55) points. The total score of professional identity was (53.61±7.90) points, and the item average score was (4.12±0.61) points. The internship satisfaction of the public unit was higher than that of the private unit, and the difference was statistically significant (P<0.001). There were no statistically significant differences in the internship experience with different gender, educational background, origin of students, the selection method of the internship unit (P>0.05).Internship satisfaction was positively correlated with their professional identity (r=0.587, P<0.01). Conclusion The internship satisfaction and professional identity of the interns are in the middle and high level, but there is still room for improvement. The nature of the internship unit has an impact on the internship satisfaction of interns, and the internship satisfaction has a positive moderating effect on the formation and consolidation of professional identity.