Objective To investigate the mechanism and significance of microRNA-214 (miRNA-214) targeted regulation of HOXB7 to inhibit the invasion and metastasis of colorectal cancer (CRC) cells. Methods A total of 200 inpatients with CRC admitted to Yuebei People's Hospital Affiliated to Medical College of Shantou University from January 2018 to December 2020 were selected as the research subjects, and were divided into non-metastatic group (n=100)and metastatic group (n=100) according to the occurrence of metastasis, and 200 healthy individuals during the same period were selected as the control group. Molecular biological techniques were used to detect the expression of miRNA-214, HOXB7, and Wnt/β-catenin in tissue cells, and the clinical significance was analyzed. Results The expression level of miRNA-214 in the tissues of patients of non-metastasis group and metastasis group were lower than those of the control group, the differences were statistically significant (P<0.05). The expression level of miRNA-214 in CRC SW1116 and SW480 cell lines was lower than that in normal cells, the differences were statistically significant (P<0.05). Transfection of SW1116 and SW480 cells with miRNA-214 mimics showed that high expression of miRNA-214 resulted in low expression of HOXB7. HOXB7 was the target gene of miRNA-214, and the expression level of the two was negatively correlated (r=-0.394, P<0.05). The expression level of HOXB7 in non-metastatic group and metastatic group were higher than those in control group,and the differences were statistically significant (P<0.05). The expression level of HOXB7 in SW1116 and SW480 cell lines were higher than those in normal cells, and the differences were statistically significant (P<0.05). When HOXB7 was overexpressed, Wnt/β-catenin expression was also increased, while when HOXB7 was interfered with by miRNA-214, Wnt/β-catenin expression was significantly decreased. Conclusion The miRNA-214—HOXB7—Wnt/β-catenin pathway is involved in inhibiting the invasion and metastasis of colorectal cancer, which provides a new target for the diagnosis and treatment of colorectal cancer.