血清miR- 125a 表达水平在慢性阻塞性肺疾病早期诊断中的应用价值

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摘要目的探究血清miR-125a 作为生物标志物在慢性阻塞性肺疾病（COPD）早期诊断中的价值。方法选取2017年1月～2018年6月我院收治的50 例COPD 患者作为COPD 组，另选取同期50 例正常人群作为健康对照组，50 例重度吸烟者作为重度吸烟组。收集三组的外周血清标本及临床基本资料，运用实时荧光定量法检测三组的外周血清miR-125a 表达水平，对血清miR-125a 表达进行ROC 曲线分析，并记录三组的创面愈合率。结果 COPD 组血清miR-125a 的mRNA 表达水平明显低于重度吸烟组，重度吸烟组血清miR-125a 的mRNA 表达水平显著低于健康对照组，差异有统计学意义（P＜0.05）。重度吸烟组ROC 曲线下的面积大于COPD 组，COPD 组ROC 曲线下的面积大于健康对照组，差异有统计学意义（P＜0.05）。重度吸烟组、COPD 组的创面愈合率均低于健康对照组，差异有统计学意义（P＜0.05）。结论血清miR-125a 在COPD 和重度吸烟患者中的表达水平均降低，可将血清miR-125a 作为COPD 早期诊断的生物标志物，为预防提供依据。

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关键词 ：慢性阻塞性肺疾病, miR-125a, 生物标志物, 早期诊断

Abstract： Objective To investigate the value of serum miR-125a as a biomarker in the early diagnosis of chronic obstructive pulmonary disease (COPD).Methods A total of 50 patients with COPD who were admitted to our hospital from January 2017 to June 2018 were selected as the COPD group.Another 50 normal people were selected as the healthy control group and 50 severe smokers were used as the severe smoking group.The peripheral serum samples and clinical basic data were collected of three groups.The expression levels of miR-125a in peripheral blood of the three groups were detected by real-time fluorescence quantitative method, the ROC curve of serum miR-125a expression was analyzed and the wound healing rate of the three groups was recorded.Results The mRNA expression level of serum miR-125a in the COPD group was significantly lower than that in the severe smoking group, the mRNA expression level of miR-125a in the severe smoking group was significantly lower than that in the healthy control group, and the differences were statistically significant (P＜0.05).The area under the ROC curve of the severe smoking group was larger than that of the COPD group, and the area under the ROC curve of the COPD group was larger than that of the healthy control group, with statistically significant differences (P＜0.05).The wound healing rate of the severe smoking group and the COPD group was lower than that of the healthy control group, and the difference was statistically significant(P＜0.05).Conclusion The expression levels of serum miR-125a decrease in patients with COPD and severe smoking,and serum miR-125a can be used as a biomarker for the early diagnosis of COPD to provide a basis for prevention.

Key words： Chronic obstructive pulmonary disease miR-125a Biomarkers Early diagnosis

基金资助:江西省卫生计生委科技计划项目（20187247）

引用本文:

刘士平；王晓鹏；匡秋江；廖晓红；张荣山. 血清miR- 125a 表达水平在慢性阻塞性肺疾病早期诊断中的应用价值[J]. 中国当代医药, 2019, 26(12): 8-11.
LIU Shi-ping WANG Xiao-peng KUANG Qiu-jiang LIAO Xiao-hong ZHANG Rong-shan. Application value of serum miR-125a expression level in the early diagnosis of chronic obstructive pulmonary disease. 中国当代医药, 2019, 26(12): 8-11.

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