cTfh细胞及相关miR-146a在乙肝疫苗记忆性免疫应答中的动态表达及关联研究

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摘要目的检测乙肝疫苗记忆性免疫应答过程中miR-146a与外周血滤泡辅助性T细胞（cTfh）动态表达水平，分析miR-146a、Tfh与乙肝疫苗免疫应答之间的相关性。方法于2017年7月招募大朗社区卫生服务中心已完成3针乙肝疫苗标准程序的健康成人接种1针乙肝疫苗，收集12例健康志愿者的外周血标本。在疫苗接种后的不同时间点，采用ELISA法测定血清中乙肝表面抗体（HBsAb）浓度；采用流式细胞术检测外周血单个核细胞（PBMC）中Tfh细胞、B细胞、浆细胞、记忆性B细胞的比例；采用荧光定量PCR检测miR-146a的相对表达量。结果 cTfh在疫苗接种后第7天达到最高（P=0.001），HBsAb水平在疫苗接种后第14天达到最高，差异有统计学意义（P<0.001）。Tfh 细胞与 B 细胞成负相关（r=-0.426，P=0.03），浆细胞与 HBsAb 成正相关（r=0.330，P=0.04）。miR-146a与cTfh、HBsAb均无相关性（P＞0.05）。结论 Tfh细胞可能参与乙肝疫苗记忆性免疫应答过程，但未能证明miR-146a与乙肝疫苗免疫应答存在相关性。

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	余祖卫
	李玉莲
	陈丽华
	尹明娟
	黄玲凤
	张艳

关键词 ： miR-146a, 滤泡辅助性T细胞, 乙肝疫苗, 免疫应答, 相关性

Abstract：Objective To detect the dynamic expression levels of miR-146a and circulating T follicular helper(cTfh)cell during immune response of hepatitis B vaccination,and then analyze the association between miR-146a,cTfh cell and the immune response of hepatitis B vaccination.Methods In July 2017,a group of healthy adults who had completed the standard procedure for the hepatitis B vaccination in Community Health Service Center of Dalang Country were recruited to receive one dose of hepatitis B vaccination,and peripheral blood samples were collected from 12 volunteers at different time points after vaccination,ELISA method was used to measure the concentration of Hepatitis B surface antibody(HBsAb)in serum;Flow cytometry was used to detect the proportion of Tfh cells,B cells,plasma cells,and memory B cells in peripheral blood mononuclear cells(PBMCs).Real-time fluorescence quantitative PCR was used to detect the relative expression of miR-146a.Results cTfh cell reached the highest level(P=0.001)on the 7thday after vaccination,and the HBsAb reached the highest level on the 14thday after vaccination,and the difference was statistically significant(P<0.001).There was a negative correlation between the Tfh cells and B cells(r=-0.426,P=0.03),and a positive correlation between the plasma cells and HBsAb (r=0.330,P=0.04).There was no significant correlation between the expression level of miR-146a,cTfh cell and HBsAb (P＞0.05).Conclusion Tfh cells might play a role in the immune response to hepatitis B vaccination,but not demonstrate the association between miR-146a and the immune response of hepatitis B vaccination.

Key words： miR-146a T follicular helper cell Hepatitis B vaccination Immune response Association

基金资助:广东省东莞市社会科技发展项目（2014108101040）

通讯作者: 李玉莲（1973-），女，副主任护师

作者简介: 余祖卫（1971-），男，主任医师

引用本文:

余祖卫;李玉莲; 陈丽华;尹明娟;黄玲凤; 张艳. cTfh细胞及相关miR-146a在乙肝疫苗记忆性免疫应答中的动态表达及关联研究[J]. 中国当代医药, 2018, 25(20): 12-15.
YU Zu-wei;LI Yu-lian;CHEN Li-hua;YIN Ming-juan;HUANG Ling-feng;ZHANG Yan. Expression dynamics and association of cTfh cells and miR-146a in the immune response to hepatitis B vaccination. 中国当代医药, 2018, 25(20): 12-15.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2018/V25/I20/12

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