沈阳市区30～50岁成人上呼吸道微生态状况分析

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摘要目的了解健康成年人上呼吸道菌群多样性及优势菌群构成，为从微生态角度预防以口咽部为门户的呼吸道感染提供科学依据。方法随机选取2012年4～6月60名沈阳市区30～50岁健康成人作为研究对象，使用无菌咽拭子采集标本，对菌群进行定量分析、定性鉴定。结果60名沈阳市区30～50岁健康成人咽后壁的需氧菌群与厌氧菌群之比为2.51∶1；检出率较高的需氧菌及厌氧菌包括缓症链球菌、口腔链球菌、唾液链球菌、干燥奈瑟球菌、灰色奈瑟球菌及麻疹孪生球菌、普氏厌氧球菌、韦荣球菌、丙酸丙酸杆菌等，检出率较高的菌，其菌株构成比也较高。结论沈阳市区30～50岁健康成人咽后壁检出率和构成比都较高的需氧菌和厌氧菌共同构成上呼吸道口咽部优势菌群，在维持呼吸系统微生态固有平衡中起重要作用，进一步阐明其生态学意义，能够为呼吸道益生菌的备选菌株应用于呼吸道活菌制剂的开发研究提供理论依据。

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	徐佳
	陈魁敏
	陈冬梅
	徐静
	海晓欧
	周园
	肖纯凌

关键词 ：呼吸道, 需氧菌, 厌氧菌, 微生态, 成人

Abstract：Objective To understand the diversity of the upper respiratory tract microflora and the constitution of dominant microflora in healthy adults so as to provide a scientific basis for the prevention of respiratory tract infections via oral approach.Methods Altogether 60 healthy adults aged 30 to 50 years old in the urban area of Shenyang from April to June in 2012 were randomly selected as the objects of this research.The samples were collected through aseptic throat swab.The quantitative analysis and qualitative identification of microflora were made.Results The proportion between postpharyngeal aerobic bacteria and anaerobic bacteria in 60 healthy adults aged 30 to 50 years old in the urban area of Shenyang was 2.51∶1;the aerobic and anaerobic bacteria with higher detection rate included Streptococcus mitis, Streptococcus oralis,Streptococcus salivarius,Neisseria sicca,Neisseria cinerea and Measles twin cocci,Anaerococcus prevotii,Veillonella,Propionibacterium propionicus etc.;the bacteria with higher detection rate also had higher constituent ratio of strains.Conclusion The dominant microflora of upper respiratory tract pharynx is constituted by both the postpharyngeal aerobic bacteria and anaerobic bacteria with higher detection rate and higher constituent ratio in healthy adults aged 30 to 50 years old in the urban area of Shenyang,which plays an important role in maintaining the inherent ecological balance in the respiratory system,further clarifies its ecological significance,and provides a theoretical basis for the research on application of alternative probiotic strains in development of respiratory bacteria preparation.

Key words： Respiratory tract Aerobic bacteria Anaerobic bacteria Micro-ecology Adult

基金资助:辽宁省科学技术计划项目（2013225086）；
辽宁省沈阳市科学技术项目（F14-181-1-00）；
辽宁省沈阳市科技计划项目（F13-220-9-36）

作者简介: 徐佳（1980-），女，硕士，讲师，研究方向：环境与健康、微生物、微生态

引用本文:

徐佳;陈魁敏;陈冬梅;徐静;海晓欧;周园;肖纯凌. 沈阳市区30～50岁成人上呼吸道微生态状况分析[J]. 中国当代医药, 2017, 24(7): 156-158.
XU Jia;CHEN Kui-min;CHEN Dong-mei;XU Jing;HAI Xiao-ou;ZHOU Yuan;XIAO Chun-ling. Analysis of micro ecological status of upper respiratory tract in adults aged 30 to 50 years old in the urban area of Shenyang. 中国当代医药, 2017, 24(7): 156-158.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2017/V24/I7/156

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