Abstract：Objective To investigate the expression and significance of leukocyte-associated immunoglobulin-like receptor 1(LAIR-1)in lymphocytes of patients with cervical cancer.Methods From January 2019 to November 2020,30 patients with cervical cancer in the Department of Gynecology,the Third People′s Hospital of Jiujiang City were included in the cervical cancer group,and 30 volunteers who received physical examinations during the same period were included in the healthy group.The blood of patients in the cervical cancer group was collected before treatment and surgery.The cervical cancer group was divided into two subgroups according to the random number table method:15 cases in the stimulation group and 15 cases in the non-stimulation group.Each group had 3 multiple holes,and the following reagents were added respectively:transforming growth factor-β+polyhydroxyalkanoates(PHA);no PHA,transforming growth factor-β.The Ficoll density gradient centrifugation method was used to separate peripheral blood mononuclear cells(PBMC),the lymphocytes were circled in the FSC-SSC scatter diagram,and the expression levels of LAIR-1 were analyzed in the T cell subsets of CD4+,CD8+and natural killer(NK)cells.Results The expression levels of LAIR-1 molecules of CD4+,CD8+T cells and NK cells in the cervical cancer group were higher than those in the healthy group,and the differences were statistically significant(P＜0.05).The expression levels of LAIR-1 in CD4+,CD8+and NK cells in the stimulation group were lower than those in the non-stimulation group,and the differences were statistically significant(P＜0.05).Conclusion LAIR-1 may participate in the mechanism of inhibiting the killing activity of NK cells and CTL cells,and inhibit the body′s anti-tumor immune response.
孙艳. LAIR-1在宫颈癌患者淋巴细胞中的表达及其意义[J]. 中国当代医药, 2021, 28(15): 112-114转119.
SUN Yan. Expression and significance of LAIR-1 in lymphocytes of patients with cervical cancer. 中国当代医药, 2021, 28(15): 112-114转119.
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