Effect of Shujin Jianyao Pills on articular cartilage of New Zealand rabbit knee osteoarthritis models
LI Rui-peng1,2,3 CHEN Di-xin1 GUO Qiu-ping1 NI Qing-chun1▲ ZHOU Jiu-yao2
1. Drug Nonclinical Evaluation and Research Center of Guangzhou General Pharmaceutical Research Institute Co.,Ltd., Guangdong Province, Guangzhou 510240, China;
2. Postdoctoral Research Station of Guangzhou University of Chinese Medicine, Guangdong Province, Guangzhou 510105, China;
3. Postdoctoral Research Station of Guangzhou Pharmaceutical Group Co., Ltd., Guangdong Province, Guangzhou 510130, China
Abstract：Objective To study the effect of Shujin Jianyao Pills on articular cartilage of New Zealand rabbit knee osteoarthritis model. Methods After one week of adaptive feeding, 12 New Zealand rabbits were randomly selected as the sham operation group. The osteoarthritis models were prepared by articular cartilage defect caused by surgery in other animals. After 4 weeks of modeling, the animals were randomly divided into model group, glucosamine hydrochloride group (positive control), Shujin Jianyao Pills low, middle and high dose groups, 12 cases in each group, half male and half female. After grouping, the animals were intragastrically administrated with drugs once daily for 28 days. The therapeutic effect and mechanism of Shujin Jianyao Pills on osteoarthritis were studied by detecting the range of knee joint activity, local tenderness threshold, whole blood viscosity, inflammatory cytokines of articular cavity flushing fluid and articular cartilage histopathology. Results The knee joint range and local tenderness threshold of the waist in the Shujin Jianyao Pills middle and high dose groups were higher than those in the model group, the whole blood viscosity and interleukin 1β (IL-1β), tumor necrosis factor-α (TNF-α) and prostaglandin E2 (PGE2) levels in articular cavity were lower than those in the model group, and the differences were statistically significant (P＜0.05). The articular cartilage scores of the glucosamine hydrochloride group and the Shujin Jianyao Pills high dose group were lower than those of the model group, and the differences were statistically significant (P＜0.05). Conclusion Shujin Jianyao Pills have a certain therapeutic effect on osteoarthritis. Improving hemorheology, reducing inflammatory cytokines in articular cavity and protecting articular cartilage may be the mechanism of its action.
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