Analysis on secretion levels of long-term vaccine activation of Th1/Th2 cytokine after new influenza A(H1N1)vaccination in children
XIA Hong-bo1 CHEN Jun-ru1 GUO Jin-jin2 TANG Yan-li2 SUN Wan-bang2▲
1.Department of Child Health Care,Maternal and Child Health Care Hospital of Longgang District in Shenzhen City, Shenzhen518172,China;
2.Teaching and Research Section of Immunology,Zhuhai Campus,Zunyi Medical College, Zhuhai519041,China
Abstract：Objective To test the changes of secretion levels of long-term vaccine activation of Th1/Th2 cytokine after new influenza A(H1N1)vaccination in children.Methods A total of 31 healthy children who were on their own willing for new influenza A(H1N1)vaccination from December 2009 to January 2010 in our hospital were selected,lymphocyte cells were separated from 3 ml peripheral blood for culture,and then were divided into new influenza A(H1N1)stimulation group(the experimental group)and normal cell group(the control group).The levels of Th cytokine secretion were measured by flow CBA and enzyme-linked immunosorbent assay(ELISA).Results The technical examination of the flow CBA showed that the levels of TNF-α and IL-10 in the experimental group was significantly lower than those in the control group,with significant difference(P＜0.05).The ELISA method displayed no significant difference in the level of TNF-α and IL-4 between the experimental group and the control group(P＞0.05).The level of IL-10 in the experimental group was significantly lower than that in the control group,which was in statistical differences(P＜0.05).The levels of IL-10 in both the control group and the experimental group tested by flow CBA was significantly higher than those by the ELISA test, with statistical differences(P＜0.05).The correlation of test outcomes by the two methods was displayed a statistical significance in equation of Y=206.59+0.082X and Y=136.70+0.168X respectively.Conclusion Re-stimulation of new influenza A H1N1 vaccine cannot activate Th1 and Th2 cells in a great success,that′s to say,no satisfactory Th1 cellular immune response and humoral immunity suggesting that the specific immune memory function of the new influenza AH1N1 is poor.The higher sensitivity and the same test results are obtained by flow CBA and the traditional ELISA method in detecting cytokines.
Neuhaus V，Chichester JA，Ebensen T，et al.A new adjuvanted nanoparticle-based H1N1 influenza vaccine induced antigen-specific local mucosal and systemic immune responses after administration into the lung[J].Vaccine，2014，32（26）：3216-3222.
[7]
Korn T，Bettelli E，GaoW，et al.IL-2 initiates an alternative path2 way to induce proinflammatory TH17 cells[J].Nature，2007，448（7152）：484-487.
[8]
Nurieva R，Yang XO，Martinez G，et al.Essential autocrine regula2tion by IL-2 in the generation of inflammatory T cells[J].Nature，2007，448（7152）：480-483.
[9]
Liu Y，Yang B，Zhou M，et al.Memory IL-2 producing CD4+ T cells specific for Candida albicans are present in humans[J]. Eur J Immunol，2009，39（6）：1472-1479.
[10]
Zhou MH，Yang BY，Ma RH，et al.Memory Th217 cells specific for C.albicans are persistent in human peripheral blood[J].Immunol Lett，2008，118（1）：72-81.
[11]
Seder RA，Ahmed R.Sinilarities and differences in CD4+and CD8+effector and memory T cell generation[J].Nat Imunol Rev，2003，4（9）：835-842.
[12]
Vohra N，Verhaegen M，Martin L，et al.TNF-alpha treated DC exacerbates disease in a murine tumor metastasis model[J].Cancer Immunol Immunother，2010，59（5）：729-736.
[13]
Curtis DJ，Muresan P，Nachman S，et al.Characterization of functional antibody and memory B-cell responses to pH1N1 monovalent vaccine in HIV-infected children and youth[J].PLoS One，2015，10（3）：617-621.
[14]
Mohn GI，Bredholt G，Brokstad KA，et al.Longevity of B cell and T cell responses after live attenuated influenza vaccination in children[J].J Infect Dis，2014，211（10）：1541-1549.
[16]
Bröker BM，Holtfreter S，Bekeredjian-Ding I.Immune control of Staphylococcus aureus-regulation and counter-regulation of the adaptive immune response[J].Int J Med Microbiol，2014，304（2）：204-214.
Rodriguez-Ramirez HG，Salinas-Carmona MC，Barboza-Quintana O，et al.CD206+cell number differentiates influenza A（H1N1）pdm09 from seasonal influenza A virus in fatal cases[J].Mediators Inflamm，2014，2014：921054.
京公网安备 11010502046607号