rhEPO reduces neuron apoptosis after intracerebral hemorrhage in rats by regulating JAK2/STAT5 signaling
LIANG Jun-jun1 WANG Ya-bing2 XIN Hai-bin1 LIU Wei3▲
1.Medical College of Qingdao University in Shandong Province,Qingdao 266071,China;
2.Department of Neurosurgery,Xuanwu Hospital of Capital Medical University,Beijing 100053,China;
3.Department of Neurosurgery,Affiliated Hospital of Qingdao University Medical College in Shandong Province,Qingdao 266071,China
Abstract：ObjectiveTo investigate whether recombinant human erythropoietin(rhEPO)could decrease neuron apoptosis after cerebral hemorrhage by regulating JAK2/STAT5 signaling pathway.Methods30 Wistar male rats of 8 weeks old were randomly divided into 3 groups:sham operation group,ICH model group and rhEPO group,the rats in ICH model group were treated with ICH.In the sham operation group,the treatment were same as ICH group except blood injection.rhEPO group was injected with rhEPO at 5 min after operation.All animals were scored with neurological score at 24 h after the ICH model was established.The effect of neuronal apoptosis were detected by TUNEL staining.The expression of pro-apoptosis protein Caspase3 was detected by immunohistochemistry.The expressions of JAK2 and STAT5 mRNA and protein were measured by Real time PCR and Western blot.ResultsNeurological score was performed 24 hours after the establishment of the intracerebral hemorrhage model.According to the criteria of Longa5 standard,in ICH group,four rats were evaluated as score 1,three rats were rated as score 2 and two rats were rated as score 3.In rhEPO group,five rats were evaluated as score 1 and two rats were rated as score 2,one rat were rated at score 3,indicating that rhEPO can ameliorate neuronal damage and restore nerve function after intracerebral hemorrhage in rats.Compared to sham group,the quantity of apoptotic cells and the expression of Caspase3 in ICH model group and rhEPOgroup were significantly increased(P＜0.05),while the apoptotic cells in rhEPO group were significantly down-regulation compared with the ICH group (P＜0.05).Compared with the sham group,the protein expression and mRNA expression of JAK2 and STAT5 in ICH group and rhEPO group were significantly up-regulated (P＜0.05),while the protein expression and mRNA expression of JAK2 and STAT5 in rhEPO group were decreased significantly compared to ICH group(P＜0.05).ConclusionrhEPO reduce neuronal apoptosis by regulating JAK2/STAT5 signal after cerebral hemorrhage,and has protective effect on the nervous system in rats.
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