Establishment and quality detection of biological sample bank of cerebral apoplexy in Qiqihar
WEI Dong-mei1 YIN Gang2 LIU Rui1 WANG Qiang3 WANG Chao-nan1 QIU Xue-rong3▲
1.Individualized Clinic Center，the First Hospital of Qiqihar City，Qiqihar 161005，China；
2.Manufacturing Laboratory，Qiqihar Hospital of Traditional Chinese Medicine，Qiqihar 161000，China；
3.Department of Cerebral Apoplexy，the First Hospital of Qiqihar City，Qiqihar 161005，China
Abstract：Objective To targeted collect the biological samples of patients with cerebral apoplexy in Qiqihar，to construct a standardized biological sample bank of cerebral apoplexy with high quality and provide sample sources and data supporting platform for the data analysis and clinical decisions for cerebral apoplexy in Qiqihar.Methods The standardized operative process was made，the object was selected，blood serum，blood plasma，white cells，DNA and RNA extracted from the white cells of the standardized patients were collected and stored in the refrigerator at-80℃or liquid nitrogen.The quality of DNA and RNA was detected so that clinical sample sources with high quality were to be provided.Results From 2013，a total of 753 patients were enrolled in the sample bank.Samples of blood serum and blood plasma were 3008 respectively，white cells were 2253 and DNA and RNA were 3770 respectively.The results of electrophoresis showed the RNA electrophoresis had both 18S and 28S stripes without dispersion and the lumen of 28S stripe was as twice as that of the 18S stripe.DNA electrophoresis had clear stripes with good integrity.Conclusion The standardized biological sample banks of cerebral apoplexy with high quality in Qiqihar is primarily established to further explore and apply the data sources of cerebral apoplexy，which can provide help for individualized treatment of patients with cerebral apoplexy in the future.
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