Abstract：Objective To explore the application and effects of beyond bloodstain blood collected method in newborns disease screening. Methods The first phase:2826 newborn babies who screened in our hospital from April 2013 to June 2014 were selected retrospectively.They were divided into the experimental group A（odd numbers，n=1398）and control group A（even numbers，n=1428）according to the date of birth.In the control group A，the routine method was used to carry out blood collection.In the experimental group A，lidocaine plus heel inunction orientation oblique insertion（improved method）was used for blood.The second phase:2526 newborn babies who screened in our hospital from July 2014 to March 2015 were selected retrospectively.They were divided into the experimental group B（odd numbers，n=1398）and control group B（even numbers，n=1128）according to the date of birth.In the control group B，lidocaine plus heel inunction orientation oblique insertion（improved method）was used for blood.The experimental group B was treated with great saphenous vein pumping 0.1 ml blood drops in blood after the blood spot piece made of blood.The success rate of blood sampling and the quality of dried blood spot of filter paper were compared among the 4 groups. Results The first phrase:the rate of first grade blood，blood film pass rate，the success rate of blood sampling in the experimental group A were 88.70%，99.50%，95.00%，higher than the 41.20%，86.80%，63.00%in control group A，the difference was statistically significant（P＜0.05）.The second phrase:the rate of blood slice and the passing rate of blood in the experimental group B was 100.00%and 100.00%respectively，higher than 89.10%，99.60%in the control group B，and the difference was statistically significant（P＜0.05）.The success rate of blood collection in the control group B was 94.00%，higher than 69.00%in the experimental group B，and the difference was statistically significant（P＜0.05）.The pain of experimental group A and control group B was significantly lighter than that of the control group A and experimental group B respectively，the difference was statistically significant（P＜0.05）. Conclusion Beyond blood collected method in newborns disease screening has obvious effects，success rate of once blood collection is high and with less pain.It is worth to popularize.
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