雷奈酸锶对骨质疏松大鼠胫骨骨缺损愈合的影响

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摘要目的探讨雷奈酸锶对骨质疏松大鼠胫骨骨缺损愈合的影响。方法雌性3个月龄SD大鼠20只，随机选取5只记为伪手术组，其余15只行双侧卵巢摘除术。术后12周，随机选取5只大鼠记为模型组。处死伪手术组及模型组大鼠，测量胫骨及下颌骨牙槽突的骨密度及骨矿含量。剩余10只大鼠制造双侧胫骨骨缺损后随机将其分为两组，A组给予雷奈酸锶灌胃，B组给予等体积生理盐水灌胃。干预12周后处死两组大鼠，获取双侧胫骨及下颌骨标本，观察胫骨骨缺损愈合情况、制作组织切片检查骨缺损处成骨情况，测量下颌骨牙槽突骨密度及骨矿含量。结果术后12周，与伪手术组比较，模型组大鼠体重增加显著（P＜0.01），胫骨及牙槽突的骨密度与骨矿含量显著降低（P＜0.01）。胫骨骨缺损手术完成且药物干预12周后，可见胫骨骨缺损基本愈合，A组与B组大体观察无明显差异；HE染色结果显示，与B组相比，A组骨缺损处成骨质量更佳。A组下颌骨牙槽突骨密度及骨矿含量显著高于B组（P＜0.01）。结论雷奈酸锶对骨质疏松大鼠的骨缺损愈合有促进作用。

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	杨苗苗
	马永清

关键词 ：骨质疏松, 骨缺损, 雷奈酸锶, 胫骨, 牙槽突

Abstract：Objective To discuss the influence of strontium ranelate on tibial bone defect healing in rats with osteoporosis.Methods 30 female SD rats of 3months old were selected and randomly divided into sham operation group（n= 5）and the other 15 rates were given bilateral ovariectomy.At 12 weeks after operation，5 rats were random ly selected as model group.Rats in the sham operation group and the model group were sacrificed，and bone mineral density（BMD）and bonemineral content（BMC）of tibia andmandibular alveolar process were determined.The other 10 ratsweremade bilateral tibial bone defect and randomly divided into two groups.Rats in group A were given strontium ranelate gavage，and those in group B were given same volume of saline gavage.After 12 weeks of intervention，the rats in both groups were sacrificed to obtain their bilateral tibia and mandible model.The healing condition of tibial bone defect was observed，the osteogenesis condition of tibial bone defectwas detected by tissue sections，and BMD and BMC ofmandibular alveolar process were determined.Resu lts At 12 weeks of operation，the weight gain was significantly higher in the model group than that in the sham operation group（P＜0.01），and the BMD and BMC were significantly lower in the model group than those in the sham operation group（P＜0.01）.At 12 weeks of drug intervention after tibial bone defect operation，general healing of tibia could be observed.The general observations between group A and group B showed no significant difference.Results of HE staining showed that osteogenesis wasmore significant at bone defect in group A than that in group B，and BMD and BMC of mandibular alveolar process were significantly higher in group A than in group B（P＜0.01）.Conclusion Strontium ranelate has a positive effect on bone defecthealing in ratswith osteoporosis.

Key words： Osteoporosis Bone defect Strontium ranelate Tibial bone Alveolar process

引用本文:

杨苗苗;马永清. 雷奈酸锶对骨质疏松大鼠胫骨骨缺损愈合的影响[J]. 中国当代医药, 2016, 23(21): 11-13转18.
YANGMiao-miao;MA Yong-qing. Influence of strontium ranelate on tibial bone defect healing in rats w ith osteoporosis. 中国当代医药, 2016, 23(21): 11-13转18.

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