艾司洛尔对炎性大鼠脊髓COX-2 mRNA表达的影响

摘要
图/表
参考文献(16)
相关文章 (15)

全文: PDF (0 KB) HTML (40 KB)
输出: BibTeX | EndNote (RIS)

摘要目的探讨鞘内注射艾司洛尔对甲醛炎性大鼠脊髓背角COX-2 mRNA表达的影响。方法鞘内置管后，36只SD大鼠采用随机数学表法分为3组（n=12）：生理盐水组（N组），300 μg/kg（S1组），600 μg/kg（S2组）。鞘内置管5 d后，注射以上各组药物，然后在大鼠后肢皮下注射甲醛50 μl，以Von-Frey细丝法和Hargreaves法测定机械缩足反射阈值（MWT）和热刺激缩足反射潜伏期（TWL），观察大鼠的疼痛行为学变化。观察1.5 h后，采用RTPCR方法观察脊髓背角L4～5COX-2 mRNA表达。结果与N组比较，S1、S2组的Ⅱ相阶段的疼痛行为均有所减少，且S1、S2组L4～5节段脊髓表达的COX-2 mRNA的量均比N组少（P＜0.01）。结论鞘内注射艾司洛尔可减少甲醛大鼠脊髓背角COX-2 mRNA表达。

	服务

	把本文推荐给朋友
	加入我的书架
	加入引用管理器
	E-mail Alert
	RSS
	作者相关文章
	陈烨
	邹聪华
	郑晓春

关键词 ：疼痛, 脊髓, 艾司洛尔, 环氧化酶-2

Abstract：ObjectiveTo investigate the effect of intrathecal injection of Esmolol on the expression of COX-2 mRNA in the spinal dorsal horn of formaldehyde-induced inflammatory rats.MethodsAfter the intrathecal catheterization,36 SD rats were randomly divided into 3 groups (n=12):saline group (group N),300 μg/kg group (group S1),and 600 μg/kg group (group S2).5 days after the intrathecal catheterization,the drug of each group was given to the rats respectively and then 50 μl formaldehyde was injected subcutaneously in the hind limbs of rats.Von-Frey method and Hargreaves method were adopted to measure the mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)so as to observe the pain behavioral changes in rats.After 1.5 hours observation,RT-PCR was given to observe COX-2 mRNA expression in L4～5spinal dorsal horn.ResultsCompared with group N,phase II pain behaviors in group S1 and group S2 were reduced and the expression of COX-2 mRNA in L4～5spinal dorsal horn in group S1 and group S2 was less than that in group N (P＜0.01).ConclusionIntrathecal injection of Esmolol reduces COX-2 mRNA expression in the spinal dorsal horn of formalin-induced rats.

Key words： Pain Spinal cord Esmolol Cyclooxygenase 2

作者简介: 陈烨（1982-），主治医师，研究方向：麻醉与疼痛

引用本文:

陈烨;邹聪华;郑晓春. 艾司洛尔对炎性大鼠脊髓COX-2 mRNA表达的影响[J]. 中国当代医药, 2016, 23(35): 135-137.
CHEN Ye;ZOU Cong-hua;ZHENG Xiao-chun. Effect of Esmolol on the expression of COX-2 mRNA in spinal cord of inflammatory rats. 中国当代医药, 2016, 23(35): 135-137.

链接本文:

http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2016/V23/I35/135

[1]	李彩虹.气管表面麻醉与静注艾司洛尔对全麻诱导插管时血流动力学的影响[J].临床麻醉学杂志，2009，25（4）：1072-1073.
[2]	Lee MH，Chung MH，Han CS，et al.Comparison of effects of intraoperative esmolol and ketamine infusion on acute postoperative pain after remifentanil-based anesthesia in patients undergoing laparoscopic cholecystectomy[J].Korean J Anesthesiol，2014，66（3）：222-224.
[3]	腾士勇，陶国荣，于布为.艾司洛尔、拉贝洛尔和尼卡地平对气管插管期间心血管反应、脑电双频指数和熵指数的影响[J].临床麻醉学杂志，2008，9（3）：756-758.
[4]	Akgün Salman E，Titiz L，Akpek E，et al.Pretreatment with a very low dose of intravenous esmolol reduces propofol injection pain[J].Agri，2013，25（1）：13-18.
[5]	Dhir R，Singh MR，Kaul TK.Effect of intravenous esmolol on analgesic requirements in laparoscopic cholecystectomy[J]. Anaesthesiol Clin Pharmacol，2015，31（3）：37-39.
[6]	Yaksh TL，Rudy TA.Chronic catheterization of the spinal subarachnoid space[J].Physiol Behav，1976，17（1）：1031-1036.
[7]	Davidson EM，Doursout MF，Szmuk P，et al.Antinociceptive and cardiovascular properties of esmolol following formalin injection in rats[J].Can J Anesth，2001，48（1）：59-64.
[8]	Onifer SM，Reed WR，Sozio RS，et al.Antinociceptive effects of spinal manipulative therapy on nociceptive behavior of adult rats during the formalin test[J].Evid Based Complement Alternat Med，2015，2015：520454.
[9]	Diniz DA，Petrocchi JA，Navarro LC.Serotonin induces peripheral mechanical antihyperalgesic effects in mice[J].Eur J Pharmacol，2015，15（2）：767-769.
[10]	Hagihrra S，Taesaka N，Yoshiya I.Inhalation anesthetics sμppress the expression of c-Fos protein evoked by noxioμs somatic stimulation in the deeper of the spinal cord in the rat[J].Brain Res，1997，751（1）：124-130.
[11]	Hunter D，Chai C，Barr GA.Effects of COX inhibition and LPS on formalin induced pain in the infant rat[J].Dev Neurobiol，2015，75（10）：1068-1079.
[12]	Louati K，Berenbaum F.Fatigue in chronic inflammation-a link to pain pathways[J].Arthritis Res Ther，2015，17（2）：254-256.
[13]	Ji RR，Gereau RW 4th，Malcangio M，et al.MAP kinase and pain[J].Brain Res Rev，2009，60（1）：135-148.
[14]	Mizobuchi S，Matsuoka Y，Obata N，et al.Antinociceptive effects of intrathecal landiolol injection in a rat formalin pain model[J].Acta Med Okayama，2012，66（3）：285-289.
[15]	Gencer A，Gunduz O，Ulugol A.Involvement of descending serotonergic and noradrenergic systems and their spinal receptor subtypes in the antinociceptive effect of dipyrone[J]. Drug Res（Stuttg），2015，65（12）：645-649.
[16]	Hageluken A，Naurnberg B，Harhammer R，et al.Lipophilic beta-adrenoceptor antagonists are effective direct activators of G-proteins[J].Biochem Pharmacol，1994，47（10）：1789-1795.