Relationship between plasma circulating microRNA-328-3p and rheumatic heart disease with atrial fibrillation
TANG Chun-shi1 LU Xin-lin1 TAN Li-hui2 CHEN Wen-jiang3▲
1.Department of Cardiovascular Medicine,Chenzhou Fourth People′s Hospital,Hu′nan Province,Chenzhou 423000,China;
2.Department of Emergency,Chenzhou Fourth People′s Hospital,Hu′nan Province,Chenzhou 423000,China;
3.Department of Cardiovascular Medicine,the Second Hospital Affiliated to Chongqing Medical University,Chongqing 400010,China
Abstract：Objective To investigate whether plasma circulating microRNA-328-3p is related to the occurrence of rheumatic heart disease with atrial fibrillation,and to explore its pathogenesis.Methods A total of 19 patients with rheumatic heart disease and atrial fibrillation from August 2017 to July 2018 were selected as observation group and 21 patients with sinus rhythm of rheumatic heart disease were selected as control group.The expression of plasma circulating microRNA-328-3p was detected by qRT-PCR.The ROC curve of the relative expression of plasma circulating microRNA-328-3p was analyzed.Bioinformatics software such as mirFocus and starBase were used to predict the bioinformatics of microRNA-328-3p.Results The expression level of plasma circulating microRNA-328-3p of the observation group was 0.31±0.05,lower than 0.69±0.03 in the control group,the difference was statistically significant(P<0.01).ROC curve analysis showed that the area under the plasma circulating miR-328-3p curve was 0.9323,95%confidence interval was 0.8598-1.0005 (P<0.01).Bioinformatics predictions showed that the KEGG signaling pathways of microRNA-328-3p were mainly related to cell division,binding protein,ventricular formation,insulin receptor signaling pathway,PI3K pathway,Wnt pathway and so on.The target genes are TLE1,STT3A,USP37,EN2,NRIP1,FOXO4 and so on.Conclusion Plasma circulating microRNA-328-3p may be involved in the occurrence of rheumatic heart disease with atrial fibrillation,and can be used as a biomarker of rheumatic heart disease with atrial fibrillation.It may lead to the occurrence of rheumatic heart disease with atrial fibrillation by regulating PI3K pathway and Wnt pathway.
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