Abstract：Objective To investigate the ubiquitin protein FAT10 abnormal expression in hepatoma cells and activation of function and its molecular mechanism.Methods 38 cases of liver cancer rats were randomly divided into blank group，pcDNA3.1-FAT10 group，FAT10 siRNA group.The expression level of FAT10 mRNA was detected by RT-PCR method.After respectively FAT10 siRNA and pcDNA3.1-FAT10 recombinant expression vector transfected into human hepatoma SMMC-7721 cells by electroporation，and flow cytometry to detect cell cycle and apoptosis，RT-PCR detection of PI3K/Akt pathway.Results FAT10 mRNA in rats hepatocellular carcinoma relative expression levels were significantly higher than adjacent tissues（P＜0.05），after gene silencing in cancer cells FAT10 expression of cell cycle distribution had no significant impact on the rate of increase in apoptosis，PI3K/Akt path expression signals down；after FAT10 promote gene expression in cells，no significant effect on the cell cycle，apoptosis was significantly decreased，PI3K/Akt pathway upregulation.Conclusion FAT10 can increase the expression of PI3K/Akt pathway signal，reduce apoptosis，enhanced invasive ability of liver cancer cells，FAT10may become targets for prevention and treatment.
白东. 类泛素蛋白FAT 10在肝癌中的表达及生物学作用[J]. 中国当代医药, 2016, 23(25): 16-18.
BAIDong. Expression and biological function of ubiquitin-like protein FAT10 in liver cancer. 中国当代医药, 2016, 23(25): 16-18.
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