血管生成素-1对脂多糖所致肺损伤和炎症反应的影响

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摘要目的　探讨血管生成素-1（Ang-1）在脂多糖所致肺损伤和炎症反应中的保护作用。方法　将30只雄性KM小鼠随机分为对照组，LPS组，Ang-1组，每组10只，经气管内滴入脂多糖构建急性肺损伤（ALI）模型，建模前24 h经尾静脉注入带绿色荧光蛋白的重组腺病毒　（Ad-GFP，LPS组）或带人Ang-1的重组腺病毒　（Ad-GFP-Ag-1，Ang-1组），对照组给予等量生理盐水。建立ALI 24 h后处死全部小鼠，测定肺湿干比，观察组织形态学变化，酶联免疫吸附试验检测血清白细胞介素-6（IL-6）水平及肺泡灌洗液中粒-巨噬细胞集落刺激因子（GM-CSF）水平。结果Ang-1组肺湿干比为4.05±0.16，明显低于LPS组的5.46±0.07；Ang-1组血清IL-6水平为（173.07±10.05）ng/ml，明显低于LPS组的（324.61±8.92）ng/ml；Ang-1组肺泡灌洗液GM-CSF水平为（15.05±1.21）ng/ml，明显高于LPS组的（10.24±1.35）ng/ml；与LPS组比较，Ang-1组的肺组织病理学损伤明显减轻。结论Ang-1能减轻LPS所致的肺组织损伤和炎症反应。

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	王莹

关键词 ：血管生成素-1, 肺损伤, 脂多糖, 炎症反应

Abstract：Objective To investigate the protective effects of application of Angiotensin-1（Ang-1）on lung injury and inflammatory responses induced by lipopolysaccharide（LPS）.Methods Altogether 30male KM mice were randomly divided into three groups（control group，LPSgroup and Ang-1 group），with 10mice in each group.Intratracheal instillation of LPSwas performed so as to constructan acute lung injury（ALI）model.Either recombinant adenovirus-green fluorescent protein（LPSgroup）or recombinant adenovirus-human angiotensin 1 was intravenously injected intomice by vena caudalis 24 h beforemodeling；control group was given normal saline.All themice were sacrificed 24 h after the establishment of ALI so as to determine the Wet-Dry（W/D）ratio of lung，the histological changes of lung tissues was observed and serum interleukin-6（IL-6）level and granulocytemacrophage-colony stimulating factor（GM-CSF）level in bronchoalveolar lavage fluid（BALF）according to ELISA were detected.Results TheW/D ratio of lung and serum IL-6 level of Ang-1 group were（4.05±0.16）and（173.07±10.05）ng/ml，significantly lower than（5.46±0.07）and（324.61± 8.92）ng/ml in LPS group；the GM-CSF level in BALF of Ang-1 group was（15.05±1.21）ng/ml，significantly higher than that in LPSgroup（10.24±1.35）ng/ml.At the same time，the pathological injury of lung tissues in Ang-1 group was significantly reduced compared with LPS group.Conclusion Ang-1 can reduce the lung tissue damage and inflammatory responses induced by LPS.

Key words： Angiotensin-1 Lung injury Lipopolysaccharide Inflammatory responses

基金资助:河南省科技厅科技计划项目（142300410129）

作者简介: 王莹，硕士研究生导师，副主任医师

引用本文:

王莹. 血管生成素-1对脂多糖所致肺损伤和炎症反应的影响[J]. 中国当代医药, 2016, 23(25): 19-21转31.
WANG Ying. Effects of Angiotensin-1 on lung injury and inflammatory pesponses induced by lipopolysaccharide. 中国当代医药, 2016, 23(25): 19-21转31.

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