UPLC method in the determination of the content of Vitamin E in plasma
ZHU Jiang1 HE Wen-ji2 LI Cui-lian2 GUO Gui-tang2 YANG Guang-neng2 ZHANG Zhen2▲
1.Department of Pharmacy,the First Affiliated Hospital of Kunming Medical University in Yunnan Province,Kunming 650032,China;
2.Kunming Children′s Hospital in Yunnan Province,Kunming 650034,China
Abstract：ObjectiveTo establish a rapid and accurate method in the determination of the content of Vitamin E in children′s plasma clinically.Methods200 μl children′s plasma was collected.140 μl methanol and isopropanol were added with the proportion of v/v,8:2,which were vortexed for 30 s.1 ml n-hexane was added and vortexed for 1 min.They were centrifuged at a high speed at room temperature for 120 s(12 000×g).700 μl supernatant was collected after centrifuge,blown dry and redissolved by 100 μl methanol.Chromatographic condition:BEH C18chromatographic column from Waters company was applied (50 mm×2.1 mm,1.7 μm).The prepared solution of 5 μl was taken as a sample,and determined with the flow rate of 0.3 mL/min.Methanol-water(96:4)was the mobile phase.The column temperature was 30℃.Under the condition,the determination wavelength was 291 nm.ResultsThe chromatographic peak of vitamin E in plasma was separated favorably,and non-interference linear regression equation was y=118296x+11644,r2=0.9997.The recovery rate was 90%-120%.ConclusionThe method is simple to operate,which is suitable for the rapid determination of Vitamin E content in children′s plasma.
朱江;何文姬;李翠莲;郭贵唐;杨光能;张桢. UPLC法测定血浆中维生素E的含量[J]. 中国当代医药, 2018, 25(3): 15-17.
ZHU Jiang;HE Wen-ji;LI Cui-lian;GUO Gui-tang;YANG Guang-neng;ZHANG Zhen. UPLC method in the determination of the content of Vitamin E in plasma. 中国当代医药, 2018, 25(3): 15-17.
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