Effect of triptolide on human renal carcinoma A498 cells proliferation,migration and invasion
CAO Bo1 FU Gang2 WANG Xiao-min3 CHAI Chun-xiang1 ZHANG Zhao-guang4 CHEN Xue-xun5▲
1.Department of Emergency, Affiliated Hospital of Weifang Medical College, Shandong Province, Weifang 261041,China;
2.Department of Urinary Surgery, Weifang Yidu Central Hospital, Shandong Province, Qingzhou 262500, China;
3.Department of Pathology, Weifang Yidu Central Hospital, Shandong Province, Qingzhou 262500, China;
4.Department of Ultrasound, Affiliated Hospital of Weifang Medical College, Shandong Province, Weifang 261041, China;
5.Department of Nephrology, Affiliated Hospital of Weifang Medical College, Shandong Province, Weifang 261041,China
Abstract：ective To investigate the effect of triptolide on the proliferation, migration and invasion of human renal carcinoma A498 cells.Methods A498 cells exposed to different concentrations of triptolide.The concentrations of triptolide were 0, 200, and 400 nmol/L, which were defined as control group, low-dose group and high-dose group.Western Blot was used for testing the expressions of AKT, p-AKT, MTOR and p-MTOR in A498 cells, and the proliferation, migration and invasion were evaluated.Results The expressions of p-AKT and p-MTOR in the low-dose group and highdose group were lower than those in the control group, the expressions of p-AKT and p-MTOR in the high-dose group were lower than those in the low-dose group, and the differences were statistically significant (P＜0.05).The proliferation,migration and invasion ability of A498 cells in the low-dose group and high-dose group were lower than those of the control group, the proliferation, migration and invasion ability of A498 cells in the high-dose group were lower than those of the low-dose group, and the differences were statistically significant (P＜0.05).Conclusion Triptolide can inhibit the proliferation, migration and invasion of A498 cells by blocking the PI3K/AKT/mTOR signal pathway.
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