The influence of Endostatin 30 peptide on proliferative activity and adhesion ability of HepG2 cell
YU Jia-qi1 ZHAO Hang1 YANG Yang1 WEI Xing-peng1 NIU Shu-dong2 LI Shu-yan3▲
1.Qiqihar Medical School,Heilongjiang Province,Qiqihar 161006,China;
2.Department of Physiology,Qiqihar Medical School,Heilongjiang Province,Qiqihar 161006,China;
3.Department of Biochemistry,Qiqihar Medical School,Heilongjiang Province,Qiqihar 161006,China
Abstract：Objective To investigate the influence of Endostatin 30 peptide on proliferative activity and adhesion ability of HepG2 cell for liver cancer.Methods The nucleotide sequences corresponding to amino-terminal 1-31 amino acids of endostatin(change the 25-31 bit sequence from RGIRGAD to RGDRGD)were linked to plasmid pTYB2,then converted to the expression of Escherichia coli BL21 (DE3)and synthesize 30 peptides composed of 30 amino acids.Results After HepG2 cells were treated by 30 peptide of 0,20,40,60,80,100 μg/ml for 24,48 h,the proliferative activity and adhesion of cells were inhibited in time and dose dependent,and compared with the control group,there was a significant difference (P＜0.05).Conclusion Endostatin 30 peptide can effectively inhibit the proliferation and adhesion of HepG2 cell,which is expected to become one of the adjuvant therapies for liver cancer.
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