Fingerprint and multicomponent quantitative analysis of Banxia Baizhu Tianma Decoction by HPLC
WANG Shu-ling1 YANG Hui-min1 LI Min-hui1 XU Nan2 MEN Lian-hui1 WANG Yue1 GU Yu-xin1 BAI Xue1 TIAN Chao1#br#
1.Medicine College of Hangzhou Normal University, Zhejiang Province, Hangzhou 310012, China;
2.Shandong Research Academy of Traditional Chinese Medicine, Shandong Province, Ji′ nan 250014, China
Abstract：Objective To establish HPLC fingerprint of Banxia Baizhu Tianma Decoction, and simultaneously determine the contents of seven components (gastrodin, liquiritin, naringin, hesperidin, isoliquiritin, atractylodes lactone Ⅲ, glycyrrhetinic acid).To provide the basis for the quality control of Banxia Baizhu Tianma Decoction.Methods The separation was carried out on an Inert Sustain C18 column (250 mm×4.6 mm, 5 μm).The mobile phase was acetonitrile-water solution, gradient elution was used.Volume flow rate was 1.0 mL/min.Detection wavelength was 254 nm.Column temperature was 30 ℃.HPLC fingerprints of 18 batches of Banxia Baizhu Tianma Decoction samples were established,the similarity was evaluated.The common peaks were assigned and identified, and the quantity of components identified was determined.Results The similarity of fingerprints of the control and 18 batches of samples were greater than 0.983.And 25 common peaks were calibrated, including 2 from Pinellia Ternata, 1 from Gastrodia Elata, 6 from Atractylodes Macrocephala, 6 from Exocarpium Citri Rubrum, 2 from Poria Cocos and 5 from Glycyrrhiza Uralensis Fisch, of which 2 common peaks could not be clearly identified.Peak 8 was gastrodin.Peak 14 was liquiritin.Peak 15 was naringin.Peak 16 was hesperidin.Peak 19 was isoliquiritin.Peak 21 was atractylodes lactone Ⅲ.Peak 25 was glycyrrhetinic acid.Contents of the seven components respectively were: gastrodin 2.1933-5.0626 mg/g, liquiritin 0.1220-0.2867 mg/g, naringin 7.3165-15.8486 mg/g, hesperidin 0.1301-0.3928 mg/g, isoliquiritin 0.1733-0.4524 mg/g, atractylodes lactone Ⅲ0.2361-0.7849 mg/g, glycyrrhetinic acid 0.0180-0.0372 mg/g.Conclusion HPLC fingerprint and quantitative determination method of Banxia Baizhu Tianma Decoction have merits of good repeatability and stability, which provide the reference for quality control and evaluation.
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