Content determination of jujuboside A,jujuboside B,spinosin and 6′′′-ferulic acid spinosin in fried Ziziphi Spinosae Semen from different habitats by UPLC-UV-ELSD
GAO Hong-ning1 YIN Yi2 YAN Guo-jun
1.College of Pharmacy,Nanjing University of Chinese Medicine,Jiangsu Province,Nanjing 210046,China；
2.Department of Research and Development,Nanjing Duokang Trading Co.,Ltd,Jiangsu Province,Nanjing 210000,China
Abstract：Objective To establish an ultra-high performance liquid chromatography (UPLC)-ultraviolet (UV)-evaporative light scattering detection (ELSD) method for the determination content of jujuboside A,jujuboside B,spinosin and 6′′′-ferulic acid spinosin in fried Ziziphi Spinosae Semen from different habitats，the quality of Fried Ziziphi Spinosae Semen from different habitats was evaluated.Methods The chromatographic separation was achieved on Waters Acquity UPLC system,UV detector,and ELSD detector,performed on ACQUITY C18 （150 mm×2.1 mm,1.7 μm）,and the mobile phase was 0.1% formic acid water-acetonitrile(gradient elution),the flow rate was 0.3 mL/min,the column temperature was 35℃,UV the detection wavelength was 335 nm and the injection volume was 3 μL,sprayer parameter was 50%,ELSD condition pressure was 172 kPa and drift tube temperature was 60℃.Results Under the above chromatographic conditions,jujuboside A,jujuboside B,spinosin and 6′′′-ferulic acid spinosin had a good linear relationship respectively with the chromatographic peak area at 27.20-435.20 mg/mL、12.60-200.8 mg/mL、26.38-422.00 mg/mL、24.38-390.00 mg/mL,the average recovery were 100.01%、99.99%、100.00%、100.04% respectively,relative standard deviation were 0.17%,0.32%,0.32%,0.32%,respectively.Conclusion UPLC-UV-ELSD method is accurate and sensitive,can be used as a quality control method of fried Ziziphi Spinosae Semen from different habitats.The results of determination show that the contents of the three components in fried Ziziphi Spinosae Semen from Hebei is relatively higher.
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