Mechanism of Toll-like receptors 3 on heart ischemia/reperfusion injury in rats
WANG Yuan-yuan1 CAO Jian2▲ DENG Li3
1.Department of Cardiology,the third hospital of Nanchang,Jiangxi Province,Nanchang 330009,China;
2.Department of Anesthesiology,the Second Affiliated Hospital of Nanchang University,Jiangxi Province,Nanchang 330006,China;
3.Science and Education Section,the third hospital of Nanchang,Jiangxi Province,Nanchang 330009,China
Abstract：ObjectiveTo investigate the expression of Toll－like receptors 3 and the correlation mechanism of ischemia/ reperfusioninjuryinrats.MethodsTOLL－likereceptor3deficient(TLR3－/－)andwildtype(WT)ratsweresubjected to ischemia/ reperfusion induced by ligation of the left anterior descending coronary artery.Sixty rats were divided into 4 groups: sham－operated group(WT)(n=15),myocardial ischemia/reperfusion group(WT)(n=15),sham－operated group(TLR3－/－) (n=15)and myocardial ischemia/reperfusion group(TLR3－/－)(n=15).The MDA,GSH,CK－MB,LVEDV,LVESV,LVEF and the expression of TLR3,NF－κB in four groups were detecte.ResultsThe level of MDA [(9.72±6.15)nmol/L],CK－MB [(1075.76±3.27)U/L]in myocardial ischemia/reperfusion group(TLR3－/－)were lower than the myocardial ischemia/reperfusion group(WT)[(14.85±5.2)nmol/L，(1842.16±4.28)U/L],the value of GSH in myocardial ischemia/reperfusion group(TLR3－/－)was higher than the myocardial ischemia/reperfusion group(WT),and the differences were statistically significant(P＜0.05).The LVEDV [(602.0±2.7)μl],LVESV [(516.0±2.9)μl]of the myocardial ischemia/reperfusion group (TLR3－/－)were lower than the myocardial ischemia/reperfusion group(WT)[(702.0±4.5)μl,(516.0±2.9)μl],the LVEF[(42.0±2.8)%]of the myocardial ischemia/reperfusion group (TLR3－/－)was higher than the myocardial ischemia/reperfusion group(WT) [(36.0±4.2)%],and the differences were statistically significant(P＜0.05).The expression of TLR3(0.862±0.158), NF－κB(0.786±1.784)of the myocardial ischemia/reperfusion group(WT)were higher than the sham－operated group(WT) (0.315±1.834,0.206±1.924),and the differences were statistically significant(P＜0.05).The expression of TLR3(0.076± 1.52)、NF－κB (0.625±2.824)of the myocardial ischemia/reperfusion group (TLR3－/－)were lower than the myocardial ischemia/reperfusion group(WT),and the differences were statistically significant(P＜0.05).Conclusion The TLR3 deficient is important to reduce myocardial I/R injury,it is related to suppress the inflammatory response by down－regulation of NF－κB signaling pathway.
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