摘要目的探讨阿格列汀联合格列美脲对2型糖尿病大鼠的降糖疗效及对胰岛β细胞功能的影响。方法将50只采用高糖高脂饮食喂养和小剂量注射链脲佐菌素建立的雄性糖尿病大鼠模型随机分为5组，即：2型糖尿病模型对照组（A组），阿格列汀单药组（B组），格列美脲单药组（C组），阿格列汀联合格列美脲组（D组）及阿格列汀联合格列苯脲组对照组（E组），每组各10只大鼠。另取10只正常大鼠作为正常对照组（F组），口服给药，每日固定时间给药1次。观察连续给药28 d后各组大鼠的空腹血糖（FBG）、血浆胰岛素水平（FIns）、甘油三酯（TG）、总胆固醇（TC）的变化，计算HOMA-βF指数及胰岛素抵抗指数（HOMA-IR）。结果经过28 d的治疗后，A、B、C、D及E组大鼠的 FBG、TG、TC、FIns、HOMA-IR 均高于 F 组，HOMA-βF 低于 F 组，差异均有统计学意义（P<0.01）。B、C、D和E组大鼠的FBG、TG、TC、FIns、HOMA-IR均显著低于A组，HOMA-βF高于A组，差异均有统计学意义（P<0.01）。 C 组大鼠的 FBG、FIns低于 B 组，TG、TC 高于 B 组，差异有统计学意义（P<0.05），D、E 组大鼠的 FBG、TG、TC、FIns、HOMA-IR均显著低于B组，HOMA-βF高于B组，差异均有统计学意义（P<0.01）。D、E组大鼠的FBG、TG、TC、FIns、HOMA-IR均低于C组，HOMA-βF高于C组，差异均有统计学意义（P<0.05）。D组和E组大鼠的各指标比较，差异无统计学意义（P>0.05）。结论分别给予阿格列汀单药、格列美脲单药或是联合用药均能显著降低2型糖尿病大鼠的血糖，改善TG、TC其胰岛β细胞功能及胰岛素抵抗，但联合用药效果优于单药治疗。
Abstract：ObjectiveTo investigate the hypoglycemic effects and the function of islet β cells of Alogliptin combined with Glimepiride in type 2 diabetes mellitus rats.Methodsfifty rats of type 2 diabetes mellitus which were induced through high-sucrose and high-fat diet combined with low-dose abdominal injection of Streptozotocin were randomly divided into five groups:model group(group A),Alogliptin group(group B),Glimepiride group(group C),Alogliptin combined with Glimepiride group (group D),Alogliptin combined with Glibenclamide (group E),10 rats in each group.Another 10 normal rats were selected as normal group(group F).The fasting blood glucose(FBG),level of plasma insulin(FIns),total cholesterol(TC)and triglyceride(TG)levels were detected in rats after treated for 28 days,the HOMA-βF index and insulin resistance index(HOMA-IR)were calculated.ResultsAfter 28 d treatment,the FBG,TG,TC,Fins and HOMAIR of the rats in group A,B,C,D and E were all higher than those in group F,the HOMA-βF was lower than group F,and the differences were statistically significant(P<0.01).The FBG,TG,TC,FIns,and HOMA-IR of rats in group B,C,D and E were significantly lower than those in group A,and HOMA-βF was higher than group A,and the differences were statistically significant(P<0.01).The FBG and FIns of rats in group C were lower than those in group B,TG and TC were higher than those in group B,the differences were statistically significant(P<0.05).The FBG,TG,TC,Fins and HOMA-IR of rats in group D and E were significantly lower than those of group B,HOMA-βF was higher than group B,and the differences were statistically significant(P<0.01).The FBG,TG,TC,FIns and HOMA-IR of the rats in group D and E were lower than those in group C,HOMA-βF was higher than that in group C,and the differences were statistically significant(P<0.05).There was no statistically significant difference between D and E groups(P>0.05).ConclusionAlogliptin,Glimepiride single or combined can significantly reduce the blood glucose of type 2 diabetic rats,improve TG,TC,the function of islet β cell and insulin resistance,but combined effect is better than single drug therapy.
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