侧脑室灌注促皮质激素释放激素诱导大鼠幼鼠惊厥样行为的初步机制研究

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摘要目的探讨侧脑室灌注促皮质激素释放激素（CRH）诱导大鼠幼鼠惊厥样行为的初步机制。方法将SD大鼠幼鼠随机分成空白实验组（仅侧脑室埋植导管，n=10）、人工脑脊液灌注组（侧脑室灌注人工脑脊液，n=10）、CRH灌注组（侧脑室灌注CRH，n=10）。在SD大鼠幼鼠生后第18天，行侧脑室微量灌注实验及全细胞膜片钳检测CRH对海马锥体神经元膜电位的影响。结果 SD大鼠幼鼠侧脑室灌注CRH（5±3）μl可以诱导出惊厥样行为，其他组均未观察到惊厥行为。CRH灌洗海马脑片锥体神经元自发性放电频率为（5.67±1.75）Hz，明显快于CRH灌洗海马脑片前锥体神经元自发性放电频率［（2.23±1.12）Hz］及CRH灌洗海马脑片后锥体神经元自发性放电频率［（1.28±0.72）Hz］（P＜0.01）。结论 CRH侧脑室灌注可成功诱发大鼠幼鼠惊厥发作，而CRH增加锥体神经元兴奋性，可作为其致痫机制之一。

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关键词 ：促皮质激素释放激素, 海马, 未成熟脑, 癫痫

Abstract：Objective To investigate the preliminary mechanism study of convulsive behavior induced by corticotropin releasing hormone（CRH）infusion into lateral ventricle in immature rats.Methods All SD immature rats were randomly divided into the blank experimental group （only lateral ventricular implant catheter，n=10），the artificial cerebrospinal fluid perfusion group（lateral ventricular perfusion artificial cerebrospinal fluid，n=10），the CRH perfusion group（lateral ventricular perfusion CRH，n=10）.The SD immature rats were performed lateral ventricle infusion at 18 days after birth and using the lateral ventricular microperfusion and whole-cell patch clamp to detect the influence of CRH for membrane potential of pyramidal neurons at hippocampal.Results Convulsion behavior could be induced by CRH of（5±3）μl infusion into the lateral ventricle of SD immature rats，while the other groups were not clearly observed.The spontaneous discharge frequency of pyramidal neurons in CRH infusion in hippocampal slices was（［5.67±1.75］Hz），which was significantly faster than before CRH infusion in hippocampal slices （［2.23±1.12］Hz）and after CRH infusion in hippocampal slices（［1.28±0.72］Hz）（P＜0.01）.Conclusion CRH administered into cerebral ventricles can induce convulsive behavior in immature rats，and increasing excitability of pyramidal neurons，which can be used as the potential mechanisms.

Key words： Corticotrophin releasing hormone Hippocampus Immature brain Epilepsy

基金资助:贵州省科技计划项目（黔科合基础［2016］1091）

引用本文:

周浩;赵魏情;崔玉霞;刘开宇. 侧脑室灌注促皮质激素释放激素诱导大鼠幼鼠惊厥样行为的初步机制研究[J]. 中国当代医药, 2019, 26(6): 26-28.
ZHOU Hao ZHAO Wei-qing CUI Yu-xia LIU Kai-yu. Preliminary mechanism study of convulsive behavior induced by corticotropin releasing hormone infusion into lateral ventricle in immature rats. 中国当代医药, 2019, 26(6): 26-28.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2019/V26/I6/26

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