Radiosensitization effect of elemene on cervical cancer HeLa cells
ZENG Chun-sheng1 ZENG Hong-xue2 HUANG Zuo-chao1 LIU Lian-bin3 ZHANG Qun-gui4 XU Qing-yun1 LI Rong5YU Ying6
1.Department of Pharmacy，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China;
2.Department of Internal Medicine，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China;
3.Department of Clinical Laboratory，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China;
4.Depeartment of Radiotherapy，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China;
5.Department of Pathology，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China;
6.Department of Gynecologic Oncology，Guanzhou Tumor Hospital in Jiangxi Province，Guanzhou 341000，China
Abstract：Objective To investigate the effect of elemene on radiosensitization of cervical cancer HeLa cells and provide evidence for its clinical application in radiotherapy of cervical cancer.Methods The untreated group of elemene was the control group，and the elemene treatment group was the treatment group.The proliferation，apoptosis and cell cycle distribution of HeLa cells were detected by colony formation assay and flow cytometry.The expression of Bax and Bcl-2 protein was detected by immunofluorescence.The implanted tumor model of HeLa cells in nude mice was used to give elemene emulsion and after different doses of radiation，the body weight and volume changes of implanted tumors were calculated，and the radiosensitizing effect of elemene emulsion on HeLa cells was analyzed.Results When the concentration of elemene was ≥40 μg/ml，it could significantly inhibit the proliferation of HeLa cells and promote apoptosis，and promote the regulation of Bax expression and inhibit the expression of Bcl-2.Under different radiation doses，HeLa cells or implanted tumors in the treatment group were significantly inhibited by growth inhibition compared with the control group.Conclusions Elemene can significantly increase the radiosensitivity of HeLa cells.
Kai S，Lu JH，Hui PP，et al.Pre-clinical evaluation of cinobufotalin as a potential anti-lung cancer agent［J］.Biochem Biophys Res Commun，2014，452（3）：768-774.
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