丙泊酚对脂多糖诱导的人脐静脉内皮细胞p38 MAPK/NF-κB信号通路活性的影响

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摘要目的研究丙泊酚对脂多糖（LPS）诱导的人脐静脉血管内皮细胞（HUBECs）p38 MAPK/NF-κB信号通路活性的影响。方法将体外培养的HUBECs随机分为4组：对照组（C组）：给予脂肪乳20 mg/L；LPS组（L组）：给予LPS 10 mg/L；丙泊酚组（P 组）：给予丙泊酚 20 mg/L；丙泊酚+LPS 组（PL 组）：给予丙泊酚 20 mg/L、LPS 10 mg/L。在给予相应药物后1、2、3、6、12 h五个时间点检测p38 MAPK、p-p38 MAPK、NF-κB含量变化。结果予以给药1 h时，L 组的 p-p38 MAPK、NF-κB 灰度值分别为 10.32±0.73、60.32±5.73，与 C 组比较表达显著增加（P＜0.05）。予以给药 2 h 时，L 组的 p-p38 MAPK、NF-κB 灰度值分别为 9.23±0.53、69.23±5.53，与 C 组比较，表达显著增加（P＜0.05）；PL组的p-p38 MAPK灰度值为6.32±0.35，与 L组比较，显著降低（P＜0.05）。给药 3 h时，L组的 p-p38 MAPK、NF-κB 灰度值分别为 11.13±0.23、71.13±5.23，与 C 组比较，表达显著增加（P＜0.05）；PL 组的 p-p38 MAPK、NF-κB灰度值分别为 8.95±0.21、56.95±5.21，与 L 组比较，显著降低（P＜0.05）。给药 6 h 时，L 组的 p-p38 MAPK、NF-κB灰度值分别为 12.16±0.20、73.16±4.20，与 C 组比较，表达显著增加（P＜0.05）；PL 组的 p-p38 MAPK、NF-κB 灰度值分别为 9.65±0.32、58.65±4.32，与 L 组比较，显著降低（P＜0.05）。给药 12 h 时，L 组的 p-p38 MAPK、NF-κB 灰度值分别为 18.19±0.31、91.19±6.31，与 C 组比较，表达显著增加（P＜0.05），PL 组的 p-p38 MAPK、NF-κB 灰度值分别为12.65±0.12、63.36±5.12，与 L 组比较，显著降低（P＜0.05）。结论 LPS 刺激 HUBECs能引起 p-p38 MAPK、NF-κB 表达增加，予以丙泊酚后抑制此种增加，这可能是丙泊酚抗炎的作用机制之一。

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	汪鑫
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关键词 ：丙泊酚, 人脐静脉内皮细胞, 炎症

Abstract：bjective To study the influence of Propofol on the activity of p38,MAPK/NF-κ B signal pathway in human umbilical vein endothelial cells(HUBECs)induced by lipopolysaccharide (LPS).Methods HUBECs cultured in vitro were randomly divided into the 4 groups:control group(group C)was given Intralipid 20 mg/L,LPS group(L group)was given LPS 10 mg/L,Propofol group (P group)was given Propofol 20 mg/L,Propofol+LPS group (PL group)was given Propofol 20 mg/L and LPS 10 mg/L.The change in the content of p38 MAPK,p-p38 MAPK and NF-κB were detected at five time points of 1,2,3,6,and 12 h.Results When administered 1 h,the gray value of p-p38 MAPK and NF-κB in group L was 10.32±0.73,60.32±5.73 respectively,which increased significantly compared with group C(P＜0.05).When administered 2 h,the gray value of p-p38 MAPK and NF-κB in group L was 9.23 ±0.53,69.23±5.53,which increased significantly compared with group C (P＜0.05),and the gray value of PL group was 6.32±0.35,which was significantly lower than that of group L.Administration of 3 h,p-p38 MAPK,NF-κB gray value in group L was 11.13±0.23,71.13±5.23 respectively,which increased significantly compared with group C(P＜0.05).p-p38 MAPK,NF-κB gray value was 8.95±0.21,56.95±5.21 respectively in group PL,which decreased significantly compared with group L(P＜0.05).Administration of 6 h,p-p38 MAPK,NF-κB gray value was 12.16±0.20,73.16±4.20 respectively in group L,which increased significantly compared with group C (P＜0.05).p-p38 MAPK,NF-κB gray value was 9.65±0.32,58.65±4.32 respectively in group PL,which decreased significantly compared with the L group (P＜0.05).Administration of 12 h,p-p38 MAPK,NF-κB gray value was 18.19±0.31,91.19±6.31 respectively,which increased significantly compared with group C (P＜0.05).p-p38 MAPK,NF-κB gray value in group PL was 12.65±0.12,63.36±5.12 respectively,which decreased significantly compared with group L(P＜0.05).Conclusion LPS stimulating HUBECs can increase the expression of p-p38 MAPK and NF-κB,and inhibit this increase after Propofol injection may be one of the mechanisms of Propofol anti-inflammation.

Key words： Propofol Human umbilical vein endothelial cells Inflammation

基金资助:贵州省科技厅联合基金项目（黔科合LH字[2015]7142号）；贵州省科技厅软科学项目（黔科合基础[2016]1532-3）；贵州省人民医院青年基金项目（GZSYQN[2015]09号）

作者简介: 汪鑫（1983-），男，湖南常德人，硕士，主治医师，研究方向：临床应用基础研究

引用本文:

汪鑫; 雷晓文 ;陈丹;刘斌. 丙泊酚对脂多糖诱导的人脐静脉内皮细胞p38 MAPK/NF-κB信号通路活性的影响[J]. 中国当代医药, 2018, 25(9): 4-6转11.
WANG Xin;LEI Xiao-wen;CHEN Dan;LIU Bin. The effect of Propofol on the activation of p38 MAPK/NF-κB signal pathway in human umbilical vein endothelial cells induced by lipopolysaccharide. 中国当代医药, 2018, 25(9): 4-6转11.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2018/V25/I9/4

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