1.The Teaching Laboratory Center,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;
2.Faculty of Basic Medicine,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China
摘要目的研究脾气虚大鼠焦虑程度的变化并探讨其可能机制。方法选取雄性SD大鼠16只，体质量（200±10）g，将其随机分为对照组和脾气虚组（模型组），每组8只。旷场实验评价大鼠焦虑程度；ELISA法测定海马组织ATP和缩胆囊素-8（CCK-8）含量；免疫组化法检测海马CCKB型受体（CCKBR）表达。结果与对照组比较，模型组的焦虑程度显著降低（P＜0.01），海马组织的 ATP 含量降低（P＜0.05），海马 CCK-8 水平变化不明显（P＞0.05），但其受体表达下降（P＜0.05）。结论脾气虚时焦虑程度下降，其初步机制与海马能量不足和CCKBR表达减少有关。
Abstract：ObjectiveTo study the anxiety degree change of rats in spleen qi deficiency rat and discuss its initial mechanism.Methods16 male SD rats with the body mass of(200±10)g were selected and they were randomly divided into the control group and spleen qi deficiency group(model group),and there were 8 rats in each group.Open-field test(OFT)was used to evaluate anxiety degree of rats.ELISA was chosen to measure ATP and CCK-8 concentrations in hippocampal tissue.Immunohistrochemical method was used to check CCKBR expression in hippocampal tissue.ResultsCompared with those in the control group,rat′s anxiety was reduced(P＜0.01),hippocampal ATP level was decreased(P＜0.05),hippocampal CCK-8 concentration was unchanged(P＞0.05),hippocampal CCKBR expression was decreased(P＜0.05)in the model group.ConclusionAnxiety in spleen qi deficiency is lower and its initial mechanism might involve in decrease of hippocampal ATP level and CCKR expression.
刘文俊;柴纪严;谢鑫;吴成举;陈靖;单德红. 脾气虚大鼠焦虑样行为及其初步机制研究[J]. 中国当代医药, 2017, 24(20): 4-6.
LIU Wen-jun;CHAI Ji-yan;XIE Xin;WU Cheng-ju;CHEN Jing;SHAN De-hong. Study of anxiety-like behaviour and the initial mechanism of rats in spleen qi deficiency. 中国当代医药, 2017, 24(20): 4-6.
Hale MW，Bouwknecht JA，Spiga F，et al.Exposure to highand low-light conditions in an open-field test of anxiety increases c-Fos expression in specific subdivisions of the rat basolateral amygdaloid complex[J].Brain Res Bull，2006，71（1-3）：174-182.
Vialou V，Bagot RC，Cahill ME，et al.Prefrontal cortical circuit for depression-and anxiety-related behaviors mediated by cholecystokinin：role of ΔfosB[J].J Neurosci，2014，34（11）：3878-3887.
[3]
Kolik LG，Gudasheva TA，Seredenin SB.Role of the cholecystokinin system in anxiolytic activity of dipeptide GB-115[J].Bull Exp Biol Med，2012，153（6）：851-854.
[4]
Wang H，Spiess J，Wong PT，et al.Blockade of CRF1 and CCK2 receptors attenuated the elevated anxiety-like behavior induced by immobilization stress[J].Pharmacol Biochem Behav，2011，98（3）：362-368.
[7]
Lee SY，Foldy C，Szabadics J，et al.Cell-type-specific CCK2 receptor signaling underlies the cholecystokinin-mediated selective excitation of hippocampal parvalbumin-positive fastspiking basket cells[J].J Neurosci，2011，31（30）：10993-11002.
[8]
Sulakhiya K，Patel VK，Saxena R，et al.Effect of beta vulgaris Linn.leaves extract on anxiety-and depressive-like behavior and oxidative stress in mice after acute restraint stress[J].Pharmacognosy Res，2016，8（1）：1-7.
[9]
Prut L，Belzung C.The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors：a review[J].Eur J Pharmacol，2003，463（1-3）：3-33.
Fedotova J，Akulova V，Pivina S，et al.Modifications of anxiety-like behavior in prenatally stressed male offspring with imbalance of androgens[J].Am J Transl Res，2017，9（3）：1448-1459.
[16]
Aizawa F，Nishinaka T，Yamashita T，et al.GPR40/FFAR1 deficient mice increase noradrenaline levels in the brain and exhibit abnormal behavior[J].J Pharmacol Sci，2016，132（4）：249-254.
[17]
Zhang YL，Xing RZ，Luo XB，et al.Anxiety-like behavior and dysregulation of miR-34a in triple transgenic mice of Alzheimer's disease[J].Eur Rev Med Pharmacol Sci，2016，20（13）：2853-2862.
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