Exploration of open field test evaluating the mental fatigue of rats with spleen qi and spleen yang deficiency
LIU Wen-jun1 GAO Yu-ting2 DOU Zhi2 YU Kai-yang2 LIU Feng-zhi2 NIAN Yu-ting2 SHAN De-hong3▲
1.The Teaching Laboratory Center,Liaoning University of Traditional Chinese Medicine in Liaoning Province,Shenyang 110847,China;
2.The First Clinical College,Liaoning University of Traditional Chinese Medicine in Liaoning Province,Shenyang110847,China;
3.Faculty of Basic Medicine,Liaoning University of Traditional Chinese Medicine in Liaoning Province,Shenyang110847,China
Abstract：ObjectiveTo explore the possibility and the preliminary mechanism of open field test evaluating the mental fatigue of rats with spleen qi deficiency(SQD)and spleen yang deficiency(SYD).Methods24 SPF male SD rats were selected as objects and they randomly divided into control group,SQD and SYD group,each group had 8 rats.The OFT method was used to detect the motion distance and vertical times.Electron microscope was used to observe the mitochondrial morphology of hippocampal neurons.ATP level in hippocampal tissues of each group were measured by colorimetry.ResultsMotion distance in open field test of SQD and SYD group was shorter than that of control group,and vertical times in SQD and SYD group was fewer than that of control group,and there was a statistical difference(P<0.05).Motion distance in open field test of SYD group was shorter than that of SQD group,and vertical times in SYD group was fewer than that of SQD group,and and there was a statistical difference(P<0.01).Hippocampus neuron mitochondria in SQD and SYD group showed swelling and vacuolization,and ATP level in SQD and SYD group were significantly lesser than that in control group(P<0.01),and ATP level in SYD group was lower than that in SQD group(P<0.01).ConclusionOFT can be used to evaluate and rank the mental fatigue of rats of SQD,SYD objectively and its biological bases might related to the damage of hippocampus neuron mitochondria.
刘文俊；高宇婷；窦智；于凯洋；刘凤智；年宇婷；单德红. 探讨旷场实验评价脾气虚和脾阳虚大鼠“神疲”的可能性及初步机制[J]. 中国当代医药, 2017, 24(21): 4-06转10.
LIU Wen-jun；GAO Yu-ting；DOU Zhi；YU Kai-yang；LIU Feng-zhi；NIAN Yu-ting；SHAN De-hong. Exploration of open field test evaluating the mental fatigue of rats with spleen qi and spleen yang deficiency. 中国当代医药, 2017, 24(21): 4-06转10.
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