Study on the effect and mechanism of NF-κB/COX-2 signaling pathway in cervical cancer
HUANG Jing1 PAN Yi-yun2 ZHONG Jin-ping1 YU Ying1 LIAO Zhen-rong1 ZHOU Mao-hua3 HUANG Gang3 LIU Lian-bin3▲
1.Department of Gynecological Tumor, Ganzhou Cancer Hospital, Jiangxi Province, Ganzhou 341000, China;
2.Department of Chemotherapy, Ganzhou Cancer Hospital, Jiangxi Province, Ganzhou 341000, China;
3.Department of Clinical Laboratory, Ganzhou Cancer Hospital, Jiangxi Province, Ganzhou 341000, China
Objective To investigate the effect and potential mechanism of knocking down cyclooxygenase 2 (COX-2)on the biological characteristics of cervical cancer Hela cell line.Methods Cervical cancer Hela cells stably transfected with shRNA-COX-2 were constructed.Cell proliferation ability was detected by the CCK-8 method, cell cloning ability was detected by the clone formation method, apoptosis was detected by flow cytometry and cell migration ability was detected by Transwell.The effect of knocking down COX-2 expression on cell biological function was observed.The expression of p65 and p-p65 proteins was detected by Western Blot.Results Transfection of shRNA COX-2 induced the down-regulation of p65 and p-p65 expression, and reduced the proliferation, clonal formation and migration ability of Hela cells.Conclusion Down-regulation of COX-2 may inhibit the malignant biological characteristics of cervical cancer Hela cells by regulating the NF-κB signaling pathway, thereby inhibiting the growth of cervical cancer cells.
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