Abstract：Objective To explore the role of Exosomes in promoting brain metastasis of non-small cell lung cancer, so as to provide the valuable reference for future clinical diagnosis and treatment work.Methods The exogenous Exosomes contained in the cultured non-small cell lung cancer supernatant were observed by electron microscopy and identified by Western blot.Exosomes fluorescent staining and co-culture of receptor NHA cells wee used to determine the role form of Exosomes on the receptor NHA cells and the effect on NHA cell activity, and establish an in vitro blood-brain barrier model to verify whether Exosomes are involved in non-small cell lung cancer in the process of transferring the blood-brain barrier into the brain.Results The crExo sample obtained by the separation was circular under the electron microscope and had a diameter of 30 to 1000 nm.Western blot results showed that the excretion marker proteins CD63 and CD9 were expressed in the crExo sample, which was slightly expressed in albumin and not expressed in the endoplasmic reticulum protein marker calnexin.The detection of crExoh concentration in non-small cell lung cancer cell lines showed that the late crExo protein concentration was significantly higher than that in the early stage, and the difference was statistically significant (P<0.05).Cell migration was observed by staining, and the results showed that Exosomes was involved in the process of non-small cell lung cancer crossing the blood-brain barrier.Conclusion Exosomes plays an important role in the regulation of tumor growth, invasion and metastasis.The brain metastasis of non-small cell lung cancer is also affected by Exosomes.
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