Influence of bone marrow mesenchymal stem cells on lung ischemiareperfusion injury in mice
SUN Chang-rong1，2 HOU Nian-guo1 LI Hui1 SHUAI Xun-jun1 SUN Ming-jie1 TANG Yu-ru1 XU Tang-wen1，2 AI Deng-bin1▲
1.Department of Anesthesiology,Weifang Medical College,Shandong Province,Weifang 261053,China;
2.Department of Anesthesiology,Research Center of Clinical Anesthesiology of Qingdao City,Qingdao Municipal Hospital,Shandong Province,Qingdao 266011,China
Abstract：ObjectiveTo investigate the influence of bone marrow mesenchymal stem cells(BMSCs)on lung ischemiareperfusion injury(LIRI)in mice.MethodsBMSCs from rats were isolated,cultured and purified by the whole bone marrow acherence method.LIRI was produced by occlusion of the left hilum of lungs for 60 min followed by 240 min reperfusion.70 mice were randomly divided into 7 groups(n=10),included the sham operation group(the Sham group),the ischemia-reperfusion group(the I/R group),the BMSCs 1 group(tail vein injections of 1×106BMSCs),the BMSCs 2 group(tail vein injections of 2×106BMSCs),the BMSCs 5 group(tail vein injection of 5×106BMSCs),the BMSCs 10 group(tail vein injection of 10×106BMSCs),the BMSCs it group(endotracheal injection of 5×106BMSCs).At the end of reperfusion,blood samples were obtained for blood gas analysis and PaO2/FiO2was calculated.Then,the animals were sacrificed and lung tissues were immediately removed for determination of superoxide dismutase(SOD),tumor necrosis factor-α(TNF-α),interleukin-8 (IL-8),and myeloperoxidase (MPO)and for microscopic examination of the pathological changes of lungs which were scored.ResultsCompared with Sham group,PaO2/FiO2was significantly decreased,the pathological scores and the levels of TNF-α,IL-8,and MPO were increased,and SOD activity was decreased in I/R,BMSCs 1,BMSCs 2,BMSCs 5,BMSCs 10,BMSCs it group,the difference was statistically significant (P＜0.05).Compared with I/R group,PaO2/FiO2was significantly increased,the pathological scores and the levels of TNF-α,IL-8,and MPO in lung tissues were decreased,and SOD activity was increased in BMSCs 1,BMSCs 2,BMSCs 5,BMSCs 10,BMSCs it group,the difference was statistically significant(P＜0.05).The most obvious group is BMSCs 10,the difference was statistically significant(P＜0.05).There is no obvious difference between endotracheal injection and intravascular injection with the same concentration of BMSCs,the difference was not statistically significant(P＞0.05).ConclusionBMCS can alleviate LIRI in mice,and the responsible mechanism is related to inhibition of inflammatory responses.The lung protective effect is dose dependent.There is no significant difference between endotracheal injection and intravascular injection with the same concentration of BMSCs.