慢性心肌缺血不同造模方式的比较

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摘要目的比较两种不同慢性心肌缺血造模方式之间的差异及优劣情况。方法选取雄性ICR 小鼠30只，根据造模方式的不同分为皮下注射组、缓释泵释放组、正常对照组，每组各10只。皮下注射组于小鼠腰背部皮下注射异丙肾上腺素（ISO）30 mg/（kg·d），连续注射14 d；缓释泵释放组于小鼠腰背部皮下埋入微量缓释泵，ISO 释放量30 mg/（kg·d）；正常对照组小鼠皮下注射等剂量生理盐水。采用心肌酶谱、心脏切片TTC 染色等指标衡量小鼠的心肌缺血程度。结果皮下注射组与缓释泵释放组小鼠的造模死亡率比较，差异无统计学意义（P＞0.05）；皮下注射组与缓释泵释放组小鼠的心脏心肌肥大指数高于正常对照组，差异有统计学意义（P＜0.05）；皮下注射组与缓释泵释放组小鼠的心肌肥大指数比较，差异无统计学意义（P＞0.05）；缓释泵释放组小鼠的心肌缺血面积百分比高于皮下注射组，差异有统计学意义（P＜0.05）；缓释泵释放组小鼠的心肌酶谱各项指标和肌钙蛋白（cTnT）水平高于正常对照组，差异有统计学意义（P＜0.05）；皮下注射组小鼠的血清α-羟丁酸脱氧酶（α-HBDH）、天门冬氨酸氨基转移酶（AST）及同工酶（mAST）、cTnT水平均高于正常对照组，差异有统计学意义（P＜0.05）；皮下注射组小鼠的血清肌酸激酶（CK）及同工酶（CK-MB）、乳酸脱氧酶（LDH）水平与正常对照组比较，差异无统计学意义（P＞0.05）；缓释泵释放组与皮下注射组小鼠的心肌酶谱各项指标及cTnT水平比较，差异无统计学意义（P＞0.05）。结论皮下注射及缓释泵释放ISO 两种方式均可以造成小鼠心肌肥大，缓释泵释放则能更大程度上诱导小鼠出现心肌缺血，缓释泵释放同时在更大程度上造成了心肌功能的损伤。

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	胡柳
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关键词 ：慢性心肌缺血, 异丙肾上腺素, 缓释泵, 皮下注射, 小鼠

Abstract：Objective To compare the differences and advantages between different methods of chronic myocardial ischemia.Methods Thirty male ICR mice were selected and divided into subcutaneous injection group,sustained-release pump release group and control group according to different modeling methods,with 10 rats in each group.In the subcutaneous group,Isoproterenol (ISO) 30 mg/(kg·d) was injected subcutaneously into the lumbar back of the mice for 14 days.The release pump group was placed subcutaneously in the lumbar back of the mice.The ISO release amount was 30 mg/(kg·d).The control group was injected subcutaneously with an equal dose of physiological saline.Myocardial ischemia was measured by myocardial zymogram and heart section TTC staining.Results There was no significant difference in the mortality between the subcutaneous injection group and the sustained-release pump release group (P＞0.05).The cardiac hypertrophy index of the mice in the subcutaneous injection group and the sustained-release pump release group was higher than that in the normal control group,and the difference was statistically significant (P＜0.05).There was no significant difference in the cardiac hypertrophy index between the subcutaneous injection group and the sustained-release pump release group (P＞0.05).The myocardial ischemic area of the mice in the sustained-release pump release group was higher than that of the subcutaneous injection group,and the difference was statistically significant(P＜0.05).The myocardial enzymes and cardiac troponin T (cTnT) level of the mice in the sustained-release pump release group were higher than those in the normal control group,and the differences were statistically significant (P＜0.05).The levels of α-hydroxybutyrate deoxygenase (α-HBDH),aspartate aminotransferase (AST),isozyme (mAST) and cTnT in the subcutaneous group were higher than those in the normal control group,and the differences were statistically significant (P＜0.05).While the levels of creatine kinase (CK) and isoenzyme (CK-MB) and lactate deoxygenase(LDH) in the subcutaneous group were not significantly different from those of the normal control group (P＞0.05).There was no significant difference in myocardial zymogram and cTnT between the sustained-release pump release group and the subcutaneous injection group (P＞0.05).Conclusion Both subcutaneous injection and sustained release pump release ISO can cause myocardial hypertrophy in mice,sustained release pump can induce myocardial ischemia in mice to a greater extent,and sustained release pump also causes damage to myocardial function to a greater extent.

Key words： Chronic myocardial ischemia Isoproterenol Sustained release pump Percutaneous injection Mice

引用本文:

胡柳；吴妍；韩铨；王威. 慢性心肌缺血不同造模方式的比较[J]. 中国当代医药, 2020, 27(5): 7-11.
HU Liu； WU Yan； HAN Quan； WANG Wei. Comparison of different modeling methods of chronic myocardial ischemia. 中国当代医药, 2020, 27(5): 7-11.

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