Abstract：Objective To establelish a determination method of Schisandrin content in Shengmaiyin (Dangshen Famula).Methods High performance liquid chromatography (HPLC) was used to deternmine Schisandrin content in Shengmaiyin (Dangshen Famula).The chromatographic condition∶Agilent column (4.6 mm×250 mm, 5 μm) was used, the mobilephase consisted of methyl alcohol-water, the detection wavelength was at 250 nm, the flow ratew was 1.0 ml/min,the volume was 10 μl.Results The linear range of Schisandrin was 0.02084～0.41680 mg/ml, it had a good linear relationship with its peak area, the regression equation was Y=2×107X (r=1, n=5).The accuracy was good (RSD=0.23%),the stability was good (RSD=0.4%), and the RSD of the repeated test was 0.87%.The average recovery was 102.9%and the RSD was 0.7% (n=6).The average contents of three batches of Schisandrin in Shengmaiyin (Dangshen Famula)produced by Jingfukang Pharmaceutical Group Co., Ltd.were 0.182 mg, 0.173 mg and 0.165 mg in each Shengmaiyin(Dangshen Famula).The average RSD was 0.1%.Conclusion This method is accurate, sensive, simple and stability,and has good reproducibility.It is suitable for the determination of Schisandrin in Shengmaiyin (Dangshen Famula).
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