Abstract：Objective To study the influence of nerve growth factor(NGF)on the proliferation and apoptosis of hepatic stellate cell-T6(HSC-T6)and transforming growth factor-β1(TGF-β1)/Smad protein pathway change in HSC-T6 of rat,and explore the possible mechanism of NGF influencing HSC-T6 proliferation and apoptosis.Methods HSC-T6 of rat was cultured in vitro.HSC-T6 was incubated with different concentrations of NGF and divided into the control group(0 ng/ml NGF)and experimental group(100 ng/ml NGF).The flow cytometry was used for detection of HSC-T6 apoptosis.By XTT,the influence of NGF on the proliferation of HSC-T6 was understood.The expression of TGF-β1,Smad3 and Smad7 after NGF affecting on HSC was observed by immunocytochemistry.Results The inhibition of HSC-T6 proliferation in theexperimental group(OD valuewas0.65±0.03)wassignificantly different fromthat in the control group(OD value was 0.73±0.01)(P<0.05).The apoptosis rate of the experimental group was higher than that of the control group with a statistical difference(P<0.05).The expression of TGF-β1 and Smad3 in the experimental group was down-regulated significantly,while the expression of Smad7 was greatly up-regulated compared with those in the control group(P<0.05).Conclusion NGF can inhibit the proliferation of HSC-T6 and promote its apoptosis.The mechanism may be related to the regulation of TGF-β1/Smad protein pathway.
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