Influence of vitamin D on the mesenchymal transformation of alveolarⅡepithelial cells induced by TGF-β1in rats
QI Yun-feng1 HUO Ru-jie1 LIU Dai1 CHAO Wei-guan2 TIAN Xin-rui1▲
1.Department of Respiratory Medicine,the Second Hospital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China;
2.Department of the Science of Nuclear Medicine,the First Hospital of Shanxi Medical University,Shanxi Province,Taiyuan 030001,China
Abstract：Objective To investigate the influence of vitamin D (VD)on the mesenchymal transformation of alveolarⅡepithelial cells(RLE-6TN)induced by TGF-β1in rats.Methods RLE-6TN cells were cultured in vitro,and were divided into the control group(group A),the TGF-β1group(group B),the TGF-β1＋VD group(group C),the TGF-β1＋ICG001 group(group D),the TGF-β1＋VD＋ICG001 group(group E).The cells were treated with VD and/or VD＋ICG001 at first,and TGF-β1was added into the culture solution after 24 h,the Western blot was used to detected the α-SMA,Fibronectin (Fn)and E-cadherin protein levels after 48 h.Results Under the microscope,in group A,cells adhered to the wall and showed a paving stone with tight junctions.The gap in group B was significantly increased and the spindle was obviously deformed.The cells in groups C and group D showed more paving stone-like changes than those in group B,and the cells gap decreased.Most of the cells in the group E were mostly paved stone-like changes,the gap was smallest and morphology was close to the normal group.Western blot analysis showed that the expression levels of α-SMA and Fn in groupB,C,D,E were higher than that in group A,but the expression of E-cadherin was lower than that in group A,the differences were statistically significant(P＜0.05).The levels of α-SMA and Fn in group C,D,E were lower than that in group B,but the expression of E-cadherin was higher than that in group B,the differences were statistically significant(P＜0.05).The expression levels of α-SMA and Fn in group C and group D were higher than that in group E,but the expression of E-cadherin was lower than that in group E,and the differences were statistically significant(P＜0.05).There were no significant differences in the expression levels of α-SMA,Fn and E-cadherin between group C and group D (P＞0.05).Conclusion Vitamin D can inhibited the mesenchymal transition of alveolarⅡ epithelial cells induced by TGF-β1in rats.
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