Study on the mechanism of the active ingredients of Mongolian medicine Alagabanbu on HepG2 cells
MA Shi-qi1 DENG Xiu-ling2 WU Ya-nan2 HAN Jing-jing3 ZHAO Yang2 QIAO Yi-yun2 WANG Hai-sheng2▲
1. Departmen of Outpatient Office, Inner Mongolia Hospital of Traditional Chinese Medicine, Inner Mongolia Autonomous Region, Hohhot 010020, China;
2. Department of Biochemistry and Molecular Biology, School of Basic Medicine, Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010110, China;
3. Department of Medical Experiment Center, School of Basic Medicine, Inner Mongolia Medical University, Inner Mongolia Autonomous Region, Hohhot 010110, China
Abstract：Objective To investigate the molecular mechanism of anticancer effect of cantharidin (CTD), the active ingredient of Mongolian medicine Alagabanbu on liver cancer. Methods CTD extracted by the research group was used to study the effect of CTD on the proliferation, apoptosis, cycle and other cell functions of HepG2 cells. Transcriptome sequencing was used to study the effect on mRNA gene expression of HepG2 cells. Results MTT results showed that the inhibition of HepG2 cell proliferation by CTD was dose-time dependent. Flow cytometry analysis results showed that cantharidin can induce cell cycle arrest in G2/M phase, and Caspase 3/7 fluorescence detection experiments confirmed that it can induce HepG2 cell apoptosis. Bioinformatics analysis after transcriptome sequencing, screened out 18 significantly differentially expressed mRNA genes. Conclusion CTD of Alagagambula extract can promote apoptosis and contact cell cycle arrest to inhibit hepatocellular carcinoma cells, and further affect the biological behavior of HepG2 by affecting the differential expression of mRNA genes, which may be a target for the early prediction and treatment of hepatocellular carcinoma.
马世琦;邓秀玲; 吴亚男;韩晶晶;赵 阳;乔一芸;王海生▲. 蒙药阿拉嘎斑布有效成分对肝癌HepG2 细胞作用机制的研究[J]. 中国当代医药, 2021, 28(34): 9-12.
MA Shi-qi;DENG Xiu-ling; WU Ya-nan; HAN Jing-jing;ZHAO Yang;QIAO Yi-yun;WANG Hai-sheng▲. Study on the mechanism of the active ingredients of Mongolian medicine Alagabanbu on HepG2 cells. 中国当代医药, 2021, 28(34): 9-12.
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