紫背天葵提取物对糖尿病小鼠血管炎症的影响

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摘要目的观察紫背天葵提取物对糖尿病小鼠血管炎症的影响。方法选取2019 年6 月至8 月购得的60 只健康昆明小鼠作为研究对象，随机取6 只作为正常对照组，正常饮食，其余54 只小鼠均采用高糖高脂饲料喂养1 个月加小剂量链脲佐菌素（STZ）诱导法制备糖尿病小鼠模型，剔除未造模成功（血糖值<11.0 mmol/L）的小鼠，将剩下的造模成功小鼠分为糖尿病模型组、紫背天葵提取物不同剂量组、二甲双胍阳性对照组，每组6 只。紫背天葵提取物组分别采用0.75、1.5、3 g/kg 灌胃，二甲双胍组采用150 mg/kg 灌胃，糖尿病模型组采用生理盐水灌胃。4 周后，比较各组小鼠的空腹血糖、体重、口服糖耐量。收集小鼠血清，采用酶联免疫吸附测定（ELISA）法比较各组小鼠血清中炎症因子白介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、抗炎因子白介素-10（IL-10）的含量，采用实时荧光定量PCR 法比较小鼠胸主动脉IL-1β、TNF-α、IL-10 的表达。结果糖尿病模型组在实验结束时的血糖、口服糖耐量曲线下面积、血清IL-1β、TNF-α 含量及血管组织中IL-1β、TNF-α mRNA 表达均高于正常对照组，差异有统计学意义（P<0.05）；糖尿病模型组小鼠的体重、血清IL-10 含量及血管组织中IL-10 mRNA 表达均低于正常对照组，差异有统计学意义（P<0.05）。1.5、3 g/kg 紫背天葵提取物组及二甲双胍组在实验结束后的血糖、血糖曲线下面积、血清IL-1β、TNF-α 含量及血管组织中IL-1β、TNF-α mRNA 表达均低于糖尿病模型组，差异有统计学意义（P<0.05）；1.5、3 g/kg 紫背天葵提取物组及二甲双胍组小鼠的体重、血清IL-10 含量及血管组织中IL-10 mRNA表达高于糖尿病模型组，差异有统计学意义（P<0.05）。结论紫背天葵提取物可以抑制糖尿病小鼠血管炎症的产生。

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关键词 ：紫背天葵提取物, 糖尿病小鼠, 血管炎症, 实时荧光定量PCR

Abstract：Objective To observe the effect of extract of Begonia Fimbristipula on the vascular inflammation of diabetic mices. Methods A total of 60 healthy Kunming mice from June to August in 2019 were selected as the research objects. Six mice were randomly selected as normal control group and fed with normal diet. The other 54 mice were fed with high sugar and high fat diet for one month and induced by low-dose streptozotocin (STZ). Mice without successful modeling (blood glucose <11.0 mmol/L) were excluded, and the remaining successful modeling mice were divided into diabetic model group, different doses of Begonia Fimbristipula extract group and Metformin-positive control group, with 6 mice in each group. The extract group was given 0.75, 1.5 and 3 g/kg intragastric administration, the Metformin group was given 150 mg/kg intragastric administration, and the diabetic model group was given normal saline intragastric administration. After 4 weeks, fasting blood glucose, body weight and oral glucose tolerance were compared. The contents of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) in serum of mice in each group were compared by enzyme-linked immunosorbent assay (ELISA). The expressions of IL-1β, TNF-α and IL-10 in thoracic aorta of mice were compared by real-time fluorescence quantitative PCR. Results At the end of the experiment, blood glucose, area under oral glucose tolerance curve, contents of IL-1β and TNF-α in serum and mRNA expression of IL-1β and TNF-α in vascular tissue in diabetic model group were higher than those in normal control group, and the differences were statistically significant (P<0.05). The body weight, serum IL-10 content and IL-10 mRNA expression in vascular tissues of dia-betic model group were lower than those of normal control group, and the differences were statistically significant (P<0.05). After the experiment, blood glucose, area under blood glucose curve, contents of IL-1β and TNF-α in serum and mRNA expression of IL-1β and TNF-α in vascular tissue in 1.5 and 3 g/kg Begonia Fimbristipula extract group and Metformin group were lower than those in diabetic model group, and the differences were statistically significant (P<0.05). The body weight, serum IL-10 content and IL-10 mRNA expression in blood vessels of mice in 1.5 and 3 g/kg Begonia Fimbristipula extract group and Metformin group were higher than those in diabetic model group, and the differences were statistically significant (P<0.05). Conclusion Extract of Begonia Fimbristipula can inhibit the production of vascular inflammation in diabetic mices.

Key words： Extract of Begonia Fimbristipula Diabetic mice Vascular inflammation Real-time fluorescence quantitative PCR

基金资助:国家级大学生创新创业训练计划项目（S2019105 45011）；
湖南省自然科学基金青年项目（2018JJ3480）；
湖南省教育厅科学研究优秀青年项目（18B498）

通讯作者: 谷彬（1980-），男，湖南郴州人，硕士，副教授，主要从事心血管药理学研究。

作者简介: 刘思妤（1981-），女，湖南郴州人，硕士，副教授，主要从事中药药理学研究。

引用本文:

刘思妤;李懿;谭慧;黄甜;张宇琴;谷彬▲. 紫背天葵提取物对糖尿病小鼠血管炎症的影响[J]. 中国当代医药, 2021, 28(34): 4-8.
LIU Si-yu; LI Yi ;TAN Hui ;HUANG Tian ;ZHANG Yu-qin ;GU Bin▲. Effect of extract of Begonia Fimbristipula on the vascular inflammation of diabetic mices. 中国当代医药, 2021, 28(34): 4-8.

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http://dangdaiyiyao.com/CN/ 或 http://dangdaiyiyao.com/CN/Y2021/V28/I34/4

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