Abstract:Objective To investigate the effect of fluorescent quantitative PCR in detection of cytomegalovirus(HCMV)in urine samples of children with HCMV infection at different time intervals.Methods A total of 30 cases of infection in our hospital from September 2015 to February 2017 in Department of Pediatrics of infected children,for all children were collected in the morning urine as morning urine group,collecting urine specimens as random specimens from other time periods,the application of real-time fluorescence quantitative PCR technology in different periods of urine HCMV DNA level standard for examination,the positive detection rate was compared between the two groups.Results The results showed that morning urine group,27 cases were detected HCMV positive,the detection rate of 90.00%,random time urine group,20 cases were detected HCMV positive,the detection rate of 66.67%,compared the two groups,morning urine group was significantly higher than that of random time group,the difference was statistically significant(P<0.05).Conclusion The detection of HCMV real-time fluorescent quantitative PCR in children with infection of HCMV in children,collection of morning urine samples for testing,the positive rate is high,effectively reduce the misdiagnosis,it is worth in clinical application.
黄斌;刘素云;万丽佳;刘明;吴小秧;罗勤. 不同时段尿标本检测巨细胞病毒在小儿人巨细胞病毒感染中的应用[J]. 中国当代医药, 2018, 25(12): 119-121.
HUANG Bin;LIU Su-yun;WAN Li-jia;LIU Ming;WU Xiao-yang;LUO Qin. Detection of cytomegalovirus in children with cytomegalovirus infection by urine samples at different time intervals. 中国当代医药, 2018, 25(12): 119-121.
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