Abstract：ObjectiveTo investigate effect of cordycepin on p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.MethodsNRK52E cells were cultured in the mediun with normal glucose concentration（group NG）,high glucose concentration（group HG）and high glucose concentration+cordycepin（group HG+C）.The expression of p38MAPK and TGF-β1mRNA was measured by quantitative RT-PCR;the expression level of p-p38MAPK and TGF-β1protein was detected by Western blot.ResultsThe p38MAPK and TGF-β1mRNA in NRK52E cells were highly expressed in group HGcompared with group NG（P＜0.01）;the tendency of p-p38MAPK and TGF-β1protein expressed was accordance with expression of mRNA.However,cordycepin can significantly inhibit the activation of p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.ConclusionCordycepin can significantly inhibit the activation of p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose.
梁泽智;黄洁平. 虫草素对高糖介导大鼠肾小管上皮细胞p38MAPK通路及TGF-β1表达的影响[J]. 中国当代医药, 2017, 24(17): 4-7.
LIANG Ze-zhi;HUANG Jie-ping. Effect of cordycepin on p38MAPK pathway and TGF-β1express in NRK52E cells stimulated by high glucose. 中国当代医药, 2017, 24(17): 4-7.
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