Abstract：Objective To study the early expression level of miR-1 in AMI patients and to explore the possibility and clinical meaning of it for the early diagnosis standard for AMI.Methods 40 AMI patients，40 anginal patients and 40 health people from July 2013 to July 2014 of cardiology department in our hospital were selected.The serum samples were collected at 1，2，6，12 and 24 hours after acute chest pain.The expression of serum miR-1，CK-MB and cTnI were tested by using RT-PCR.The relationship between serum miR-1，CK-MB and cTnI was analyzed.The clinical value of serum miR-1 expression level in early diagnosis AMI was explored.Results The relative expression level of serum miR-1 in AMI patients was 1.77±0.52，obvious higher than that were respectively 0.19±0.04 and 0.57±0.09 in health people and anginal patients （P＜0.05）.After the onset of illness，the serum miR-1 increased fast and reached a peak around 12 h after the onset of chest pain；Pearson relevant analysis showed that serum miR-1 expression of AMI patients had positive relationship with CK-MB and cTnI（P＜0.05）.Conclusion MiR-1 in AMI patients with high expression，which can well reflect the AMI myocardial injury and can be a new measuring marker for AMI.
周子健;崔秀平;冯可清;马坤. 血清miR-1检测在急性心肌梗死早期诊断中的临床意义[J]. 中国当代医药, 2016, 23(15): 124-126.
ZHOU Zi-jian;CUI Xiu-ping;FENG Ke-qing;MA Kun. Clinical meaning of serum miR-1 in the early diagnosis of acute myocardium infarction. 中国当代医药, 2016, 23(15): 124-126.
Bhayani MK，Calin GA，Lai SY.Functional relevance of miRNA sequences in human disease［J］.Mutat Res，2012，731（1-2）：14-19.
［4］
David R.Small RNAs：miRNA machinery disposal［J］.Nat Rev Mol Cell Biol，2013，14（1）：4-5.
［1］
Andres E，Garcia-Campayo J，Magan P，et al.Psychiatric morbidity as a risk factor for hospital readmission for acute myocardial infarction：an 8-year follow-up study in Spain［J］. Int J Psychiatry Med，2012，44（1）：63-75.
［2］
Aundhakar SC，Mahajan SK，Mane MB，et al.Reactive thrombocytosis leading to acute myocardial infarction［J］.J Assoc Physicians India，2013，61（10）：745-747.
［5］
Blondal T，Jensby NS，Baker A，et al.Assessing sample and miRNA profile quality in serum and plasma or other biofluids［J］.Methods，2013，59（1）：S1-S6.
Duan L，Xiong X，Liu Y，et al.miRNA-1：functional roles and dysregulation in heart disease［J］.Mol Biosyst，2014，10 （11）：2775-2782.
［8］
Akeson J，Bostrm PA，Gustafson C.Study of CK-MB activity in patients with acute myocardial infarction after percutaneous coronary intervention［J］.Georgian Med News，2015，239（1）：47-51.
［9］
Gupta S，Singh KN，Bapat V，et al.Diagnosis of acute myocardial infarction：CK-MB versus cTn-T in Indian patients［J］.Indian J Clin Biochem，2008，23（1）：89-91.
［10］
Leidinger P，Backes C，Meder B，et al.The human miRNA repertoire of different blood compounds［J］.BMC Genomics，2014，15：474.
［11］
Mar-Aguilar F，Rodriguez-Padilla C，Resendez-Perez D. Use of serum-circulating miRNA profiling for the identification of breast cancer biomarkers［J］.Methods Mol Biol，2014，1165（1）：71-80.
［12］
Dong L，Li Y，Han C，et al.miRNA microarray reveals specific expression in the peripheral blood of glioblastoma patients［J］.Int J Oncol，2014，45（2）：746-756.
［13］
Devaux Y，Vausort M，Goretti E，et al.Use of circulating microRNAs to diagnose acute myocardial infarction［J］. Clin Chem，2012，58（3）：559-567.
［14］
Pan Z，Sun X，Ren J，et al.miR-1 exacerbates cardiac ischemia-reperfusion injury in mouse models［J］.PLoS One，2012，7（11）：e50515.
［15］
Cheng Y，Tan N，Yang J，et al.A translational study of circulating cell-free microRNA-1 in acute myocardial infarction［J］.Clin Sci（Lond），2010，119（2）：87-95.
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