Objective To explore the application effect of intra-spinal anesthesia and analgesia in vaginal trial delivery after cesarean section.Methods A total of 156 pregnant women undergoing vaginal trial delivery after cesarean section in Maternity and Child Health Hospital of Yangdong District in Yangjiang City from August 2019 to December 2020 were selected and divided into analgesia group(n=78)and non-analgesia group(n=78)by random envelope method.In the analgesic group,intra-spinal anesthesia was performed at 2 to 3 cm from the uterine orifice,and the non-analgesic group was not given any drugs or equipment for labor analgesia.The labor methods,labor progress,postpartum hemorrhage,and postpartum urinary retention,neonatal Apgar scores,NICU occupancy rate,and visual analogue scale(VAS)score when the uterine opening reached 5,8,10 cm were compared between the two groups.Results There were no significant differences in the progress of labor,postpartum hemorrhage,postpartum urinary retention,newborn Apgar scores(at 1,5 minutes of birth),and NICU occupancy rate between the two groups(P＞0.05).The Apgar scores of newborns in the two groups at 5 minutes of birth were higher than those at 1 minute of birth,the differences were statistically significant(P＜0.05).The VAS scores of the analgesia group were lower than those of the non-analgesia group when the uterine opening was 5,8,and 10 cm,and the differences were statistically significant(P＜0.05).The VAS score of the non-analgesic group when the uterine opening was 10 cm was higher than that at 8 cm and 5 cm,and the VAS score was higher than that at 5 cm when the uterine opening was 8 cm,the differences were statistically significant(P＜0.05).The VAS score of the analgesia group was higher than that at 5 and 8 cm when the uterine orifice opened to 10 cm,the difference was statistically significant(P＜0.05).There was no statistically significant difference in the VAS score when the uterine opening was 8 cm and 5 cm in the analgesia group(P＞0.05).Conclusion It is safe and feasible to apply intra-spinal anesthesia in vaginal trial delivery after cesarean section.It can significantly reduce the pain of labor and is worthy of application and promotion.