Mechanism of circular RNA CDR1as in mice macrophage foaming
ZHANG Han1 LI Xing1 YANG Sha-sha1 ZHAO Le1 HAN Peng-fei2▲
1.The First School of Clinical Medicine,Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China;
2.Department of Laboratory,Hongqi Hospital Affiliated to Mudanjiang Medical University,Heilongjiang Province,Mudanjiang 157011,China
Abstract：Objective To study the expression level and mechanism of circular RNA CDR1as in mice macrophage foaming.Methods Mice macrophage(RAW264.7 cells)were cultured in vitro and divided into control group and oxidized low density lipoprotein(ox-LDL)groups with different concentrations,RAW264.7 cells were respectively induced with complete medium and ox-LDL of final concentrations were 25,50 and 100 μg/mL.The intracellular lipid profile was observed by oil red O stain,and intracellular CDR1as and miR-7a-5p expression were detected by real-time fluorescence quantitative PCR.Results Compared with control group,the intracellular lipid was increased in ox-LDL induced group,and the degree of RAW264.7 cells foaming was increased with the increase of the induced concentration,the differences were statistically significant(P＜0.05).The expression level of CDR1as decreased with the increase of ox-LDL induced concentration,the expression level of miR-7a-5p increased with the increase of ox-LDL induced concentration,the differences were statistically significant(P＜0.05).The expression levels of CDR1as and miR-7a-5p were correlated in mice macrophage foaming which induced with ox-LDL(R2=0.7879,P＜0.01).Conclusion The expression of CDR1as gradually decreases during macrophage foaming induced with ox-LDL,and the sponge miR-7a-5p might be used to regulate the behavior of macrophage-derived foam cells and affect the progression of atherosclerosis.
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