Influence of Resveratrol supp lement on behavior of zebrafish larvae to acute ethanol exposure
CHEN Hao-jun1 LIN Jia2 LIQiang2 GUO Ning1▲
1.Center for Chinese Medical Therapy and Systems Biology，ShanghaiUniversity of Traditional Chinese Medicine，Shanghai 201203，China；2.Translational Medical Center for Child Development and Disease-Shanghai Key Laboratory of Birth Defect of Institute of Pediatrics，Children′s Hospital，Fudan University，Shanghai 201102，China
Abstract：Objective To explore the influence of Resveratrol supplement on behavior of zebrafish larvae to acute ethanol exposure induced by different concentrations of ethanol under varying light conditions.Methods 300Wild-type AB strain zebrafish larvae（0 day post fertilization，0 dPF）without eggmembrane were randomly divided into two groups，and were treated with system water or resveratrol for 4 days，respectively.Each group was divided into 4 teams （36 per team）at 5 dpf，and acutely exposed under alcohol（0.0%，0.5%，1.0%，2.0%）.Distancemoved perminute was recorded under alternating 1ight-dark challenges，and the data was analyzed by two-factors analysis of variance.Results Acute exposure to 0.0%and 0.5%alcohol，the locomotor activity was decreased in Resveratrol supplement-treated zebrafish larvae in darkness.In the startle response to light changes，the swimming distance ratio of Resveratrol supplement under 0.5%alcohol exposured was significantly increased.The swimming distance ratio was showed over 1 in 2.0%alcohol treated groups without Resveratrol supplement.Conclusion Resveratrol supplement in early development of zebrafish larvae can exert significant influences on the behavior changes induced by acute ethanol exposure.
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