Preparation and toxicity evaluation of triptolide microspheres for intraarticular injection
WANG Li-juan1 CHE Ke-ke2▲ ZHANG Ru-chao1
1.Pharmaceutical Analysis Teaching and Research Section of School of Pharmacy,Chongqing Medical and Pharmaceutical College,Chongqing401331,China;
2.Department of Pharmacy,Chongqing People′s Hospital,Chongqing400014,China
Abstract：Objective To evaluate the quality and toxicity of triptolide microspheres for intra-articular injection.Methods Triptolide microspheres was prepared by emulsion solvent evaporation method.Surface morphology and size of microspheres were observed by transmission electron microscope.The formation of microspheres was investigated with infrared spectrometer.Entrapment efficiency and drug loading was determined by HPLC.The release of microspheres in vitro was fitted by mathematical model.The toxicity of microspheres was evaluated using hematological parameters and biochemical indexes.Results The microspheres were spherical in shape with uniform size.The mean diameter,encapsulation efficiency and drug loading was 4.75 μm,(74.37±2.30)%and(1.53±0.27)%respectively.The release of microspheres was fit for Ritger-Peppas equation.There were no significant differences of blood test between microspheres and control group.Conclusion Triptolide microspheres might be used as a new preparation for intra-articular injection.
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