ObjectiveTo study the clinical application of Midazolam and Propofol sequential administration during the sequencing sedation of mechanical ventilation.Methods80 patients from January 2015 to February 2016 in the ICU of our hospital were selected.According to the doctors′evaluations,all the patients were received：the mechanical ventilation for>72 h or more.The subjects were divided into group P(26 cases),group M(26 cases)and group M-P(28 cases)according to the different administration methods.Group P took Propofol;group M took Midazolam;group M-P took the combined therapy of Midazolam and Propofol.During the process,the related index for three groups was compared.ResultsThe hospitalization cost for group P[(4002.35±741.01)yuan]was significantly higher than group M[(852.32±43.21)yuan]and group M-P[(902.36±54.32)yuan](P=0.000).The awakening time for group M[(7.20±1.00)h]was significantly longer than group P[(0.79±0.52)h]and group M-P[(1.00±0.25)h](P=0.000).The incidence rate of repeated dysphoria for group M was higher than group P and group M-P(χ2=4.722,P=0.094).The dosage of Propofol for group P[(7882.24±1001.50)mg]was significantly higher than group M-P[(1153.50±105.30)mg](t=35.371,P=0.000).The overall dosage of Midazolam for group M-P[(310.23±51.06)mg]was lower than group M[(428.23±70.55)mg](t=7.078,P=0.000).Before sedation,after 1 h of sedation,during the sedation,levels of MAP,HR of the group P had no significant change(P>0.05),while had significant change in the group M(P<0.05),both MAP and HR had no significant change in the group M-P in each time point(P>0.05).Before sedation,after 1 h of sedation,during the sedation,the SpO2of patients in the three groups of was in creased gradually(P<0.05).ConclusionDuring the sequencing sedation of mechanical ventilation,the Midazolam and Propofol sequential administration has an obvious effect in ICU patients,can reduce the dosage of the single sedation drugs,hospitalization costs,overall dosage and incidence rate of dysphoria.Meanwhile,the combined therapy can improve the sedation effect.